AHL15_ARATH
ID AHL15_ARATH Reviewed; 310 AA.
AC Q9M2S3;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=AT-hook motif nuclear-localized protein 15 {ECO:0000312|EMBL:FAA00286.1};
DE AltName: Full=AT-hook protein of GA feedback 2 {ECO:0000303|PubMed:17277098};
GN Name=AHL15 {ECO:0000303|PubMed:15604740};
GN Synonyms=AGF2 {ECO:0000303|PubMed:17277098};
GN OrderedLocusNames=At3g55560 {ECO:0000312|Araport:AT3G55560};
GN ORFNames=T22E16.220 {ECO:0000312|EMBL:CAB75914.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP IDENTIFICATION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=15604740; DOI=10.1007/s11103-004-3249-5;
RA Fujimoto S., Matsunaga S., Yonemura M., Uchiyama S., Azuma T., Fukui K.;
RT "Identification of a novel plant MAR DNA binding protein localized on
RT chromosomal surfaces.";
RL Plant Mol. Biol. 56:225-239(2004).
RN [7]
RP FUNCTION.
RX PubMed=17277098; DOI=10.1104/pp.106.093542;
RA Matsushita A., Furumoto T., Ishida S., Takahashi Y.;
RT "AGF1, an AT-hook protein, is necessary for the negative feedback of
RT AtGA3ox1 encoding GA 3-oxidase.";
RL Plant Physiol. 143:1152-1162(2007).
RN [8]
RP FUNCTION.
RX PubMed=20738724; DOI=10.1111/j.1744-7909.2010.00969.x;
RA Lu H., Zou Y., Feng N.;
RT "Overexpression of AHL20 negatively regulates defenses in Arabidopsis.";
RL J. Integr. Plant Biol. 52:801-808(2010).
RN [9]
RP GENE FAMILY, AND DOMAIN PPC.
RX PubMed=24218605; DOI=10.1073/pnas.1219277110;
RA Zhao J., Favero D.S., Peng H., Neff M.M.;
RT "Arabidopsis thaliana AHL family modulates hypocotyl growth redundantly by
RT interacting with each other via the PPC/DUF296 domain.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E4688-E4697(2013).
CC -!- FUNCTION: Transcription factor that specifically binds AT-rich DNA
CC sequences related to the nuclear matrix attachment regions (MARs) (By
CC similarity). Binds the DNA sequence GNFEI (GA-negative feedback element
CC I) in the GA3OX1 promoter (PubMed:17277098). Negatively regulates plant
CC innate immunity (PTI) to pathogens through the down-regulation of the
CC PAMP-triggered FRK1 expression (PubMed:20738724).
CC {ECO:0000250|UniProtKB:Q8VYJ2, ECO:0000269|PubMed:17277098,
CC ECO:0000269|PubMed:20738724}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8VYJ2}.
CC -!- DOMAIN: The PPC domain mediates interactions between AHL proteins.
CC {ECO:0000269|PubMed:24218605}.
CC -!- MISCELLANEOUS: Overexpression of AHL15 results in a decreased flg22-
CC induced expression of FRK1. {ECO:0000269|PubMed:20738724}.
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DR EMBL; AL132975; CAB75914.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79400.1; -; Genomic_DNA.
DR EMBL; BT024777; ABD59115.1; -; mRNA.
DR EMBL; AY086028; AAM63238.1; -; mRNA.
DR EMBL; BR000351; FAA00286.1; -; mRNA.
DR PIR; T47695; T47695.
DR RefSeq; NP_191115.1; NM_115413.4.
DR AlphaFoldDB; Q9M2S3; -.
DR SMR; Q9M2S3; -.
DR STRING; 3702.AT3G55560.1; -.
DR iPTMnet; Q9M2S3; -.
DR MetOSite; Q9M2S3; -.
DR PaxDb; Q9M2S3; -.
DR PRIDE; Q9M2S3; -.
DR ProteomicsDB; 244894; -.
DR EnsemblPlants; AT3G55560.1; AT3G55560.1; AT3G55560.
DR GeneID; 824721; -.
DR Gramene; AT3G55560.1; AT3G55560.1; AT3G55560.
DR KEGG; ath:AT3G55560; -.
DR Araport; AT3G55560; -.
DR TAIR; locus:2099956; AT3G55560.
DR eggNOG; ENOG502QW3T; Eukaryota.
DR HOGENOM; CLU_039808_2_0_1; -.
DR InParanoid; Q9M2S3; -.
DR OMA; CNATYER; -.
DR OrthoDB; 1286084at2759; -.
DR PhylomeDB; Q9M2S3; -.
DR PRO; PR:Q9M2S3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M2S3; baseline and differential.
DR Genevisible; Q9M2S3; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0003680; F:minor groove of adenine-thymine-rich DNA binding; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0045824; P:negative regulation of innate immune response; IMP:UniProtKB.
DR CDD; cd11378; DUF296; 1.
DR InterPro; IPR014476; AHL15-29.
DR InterPro; IPR005175; PPC_dom.
DR PANTHER; PTHR31100; PTHR31100; 1.
DR Pfam; PF03479; PCC; 1.
DR PIRSF; PIRSF016021; ESCAROLA; 1.
DR PROSITE; PS51742; PPC; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Immunity; Innate immunity; Nucleus; Plant defense;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..310
FT /note="AT-hook motif nuclear-localized protein 15"
FT /id="PRO_0000432033"
FT DOMAIN 112..251
FT /note="PPC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01078"
FT DNA_BIND 88..100
FT /note="A.T hook"
FT /evidence="ECO:0000305"
FT REGION 15..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 239..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..41
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 49..81
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 247..264
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 310 AA; 32603 MW; 0F856170E37A40CB CRC64;
MANPWWVGNV AIGGVESPVT SSAPSLHHRN SNNNNPPTMT RSDPRLDHDF TTNNSGSPNT
QTQSQEEQNS RDEQPAVEPG SGSGSTGRRP RGRPPGSKNK PKSPVVVTKE SPNSLQSHVL
EIATGADVAE SLNAFARRRG RGVSVLSGSG LVTNVTLRQP AASGGVVSLR GQFEILSMCG
AFLPTSGSPA AAAGLTIYLA GAQGQVVGGG VAGPLIASGP VIVIAATFCN ATYERLPIEE
EQQQEQPLQL EDGKKQKEEN DDNESGNNGN EGSMQPPMYN MPPNFIPNGH QMAQHDVYWG
GPPPRAPPSY
