AHL20_ARATH
ID AHL20_ARATH Reviewed; 281 AA.
AC Q8GWQ2; Q8LAV4;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=AT-hook motif nuclear-localized protein 20 {ECO:0000312|EMBL:FAA00291.1};
GN Name=AHL20 {ECO:0000303|PubMed:15604740};
GN Synonyms=RNH1 {ECO:0000303|PubMed:20738724};
GN OrderedLocusNames=At4g14465 {ECO:0000312|Araport:AT4G14465};
GN ORFNames=FCAALL.196 {ECO:0000312|EMBL:AL161539};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP IDENTIFICATION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=15604740; DOI=10.1007/s11103-004-3249-5;
RA Fujimoto S., Matsunaga S., Yonemura M., Uchiyama S., Azuma T., Fukui K.;
RT "Identification of a novel plant MAR DNA binding protein localized on
RT chromosomal surfaces.";
RL Plant Mol. Biol. 56:225-239(2004).
RN [9]
RP FUNCTION.
RX PubMed=20738724; DOI=10.1111/j.1744-7909.2010.00969.x;
RA Lu H., Zou Y., Feng N.;
RT "Overexpression of AHL20 negatively regulates defenses in Arabidopsis.";
RL J. Integr. Plant Biol. 52:801-808(2010).
RN [10]
RP GENE FAMILY, AND DOMAIN PPC.
RX PubMed=24218605; DOI=10.1073/pnas.1219277110;
RA Zhao J., Favero D.S., Peng H., Neff M.M.;
RT "Arabidopsis thaliana AHL family modulates hypocotyl growth redundantly by
RT interacting with each other via the PPC/DUF296 domain.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E4688-E4697(2013).
CC -!- FUNCTION: Transcription factor that specifically binds AT-rich DNA
CC sequences related to the nuclear matrix attachment regions (MARs) (By
CC similarity). Negatively regulates plant innate immunity (PTI) to
CC pathogens through the down-regulation of the PAMP-triggered NHO1 and
CC FRK1 expression (PubMed:20738724). {ECO:0000250|UniProtKB:Q8VYJ2,
CC ECO:0000269|PubMed:20738724}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8VYJ2}.
CC -!- DOMAIN: The PPC domain mediates interactions between AHL proteins.
CC {ECO:0000269|PubMed:24218605}.
CC -!- MISCELLANEOUS: Overexpression of AHL20 results in a decreased
CC expression of NHO1 and FRK1 and in an enhanced susceptibility to
CC virulent strain DC3000. {ECO:0000269|PubMed:20738724}.
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DR EMBL; Z97336; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL161539; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002687; AEE83448.1; -; Genomic_DNA.
DR EMBL; AK118702; BAC43296.1; -; mRNA.
DR EMBL; BT006423; AAP21231.1; -; mRNA.
DR EMBL; AK227935; BAE99903.1; -; mRNA.
DR EMBL; AY087587; AAM65129.1; -; mRNA.
DR EMBL; BR000356; FAA00291.1; -; mRNA.
DR RefSeq; NP_567432.1; NM_117526.4.
DR AlphaFoldDB; Q8GWQ2; -.
DR SMR; Q8GWQ2; -.
DR IntAct; Q8GWQ2; 2.
DR STRING; 3702.AT4G14465.1; -.
DR iPTMnet; Q8GWQ2; -.
DR PaxDb; Q8GWQ2; -.
DR PRIDE; Q8GWQ2; -.
DR ProteomicsDB; 245038; -.
DR EnsemblPlants; AT4G14465.1; AT4G14465.1; AT4G14465.
DR GeneID; 827093; -.
DR Gramene; AT4G14465.1; AT4G14465.1; AT4G14465.
DR KEGG; ath:AT4G14465; -.
DR Araport; AT4G14465; -.
DR TAIR; locus:505006460; AT4G14465.
DR eggNOG; ENOG502QRFG; Eukaryota.
DR HOGENOM; CLU_039808_2_0_1; -.
DR InParanoid; Q8GWQ2; -.
DR OMA; DAYAWTH; -.
DR OrthoDB; 1334068at2759; -.
DR PhylomeDB; Q8GWQ2; -.
DR PRO; PR:Q8GWQ2; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8GWQ2; baseline and differential.
DR Genevisible; Q8GWQ2; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0003680; F:minor groove of adenine-thymine-rich DNA binding; IBA:GO_Central.
DR GO; GO:0043621; F:protein self-association; IPI:TAIR.
DR GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:1900425; P:negative regulation of defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0045824; P:negative regulation of innate immune response; IMP:UniProtKB.
DR GO; GO:2000028; P:regulation of photoperiodism, flowering; IMP:TAIR.
DR CDD; cd11378; DUF296; 1.
DR InterPro; IPR014476; AHL15-29.
DR InterPro; IPR005175; PPC_dom.
DR PANTHER; PTHR31100; PTHR31100; 1.
DR Pfam; PF03479; PCC; 1.
DR PIRSF; PIRSF016021; ESCAROLA; 1.
DR PROSITE; PS51742; PPC; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Immunity; Innate immunity; Nucleus; Plant defense;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..281
FT /note="AT-hook motif nuclear-localized protein 20"
FT /id="PRO_0000432038"
FT DOMAIN 91..229
FT /note="PPC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01078"
FT DNA_BIND 67..79
FT /note="A.T hook"
FT /evidence="ECO:0000305"
FT REGION 43..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 216..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 281 AA; 29540 MW; 0454664A00FBB0D4 CRC64;
MANPWWTNQS GLAGMVDHSV SSGHHQNHHH QSLLTKGDLG IAMNQSQDND QDEEDDPREG
AVEVVNRRPR GRPPGSKNKP KAPIFVTRDS PNALRSHVLE ISDGSDVADT IAHFSRRRQR
GVCVLSGTGS VANVTLRQAA APGGVVSLQG RFEILSLTGA FLPGPSPPGS TGLTVYLAGV
QGQVVGGSVV GPLLAIGSVM VIAATFSNAT YERLPMEEEE DGGGSRQIHG GGDSPPRIGS
NLPDLSGMAG PGYNMPPHLI PNGAGQLGHE PYTWVHARPP Y
