AHL21_ARATH
ID AHL21_ARATH Reviewed; 285 AA.
AC O82166;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 131.
DE RecName: Full=AT-hook motif nuclear-localized protein 21 {ECO:0000312|EMBL:FAA00292.1};
DE AltName: Full=Protein GIANT KILLER {ECO:0000303|PubMed:19956801};
GN Name=AHL21 {ECO:0000303|PubMed:15604740};
GN Synonyms=GIK {ECO:0000303|PubMed:19956801};
GN OrderedLocusNames=At2g35270 {ECO:0000312|Araport:AT2G35270};
GN ORFNames=T4C15.6 {ECO:0000312|EMBL:AAC61811.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=15604740; DOI=10.1007/s11103-004-3249-5;
RA Fujimoto S., Matsunaga S., Yonemura M., Uchiyama S., Azuma T., Fukui K.;
RT "Identification of a novel plant MAR DNA binding protein localized on
RT chromosomal surfaces.";
RL Plant Mol. Biol. 56:225-239(2004).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=19956801; DOI=10.1371/journal.pbio.1000251;
RA Ng K.H., Yu H., Ito T.;
RT "AGAMOUS controls GIANT KILLER, a multifunctional chromatin modifier in
RT reproductive organ patterning and differentiation.";
RL PLoS Biol. 7:E1000251-E1000251(2009).
RN [5]
RP REVIEW.
RX PubMed=20173412; DOI=10.4161/psb.5.2.11111;
RA Ng K.H., Ito T.;
RT "Shedding light on the role of AT-hook/PPC domain protein in Arabidopsis
RT thaliana.";
RL Plant Signal. Behav. 5:200-201(2010).
RN [6]
RP GENE FAMILY, AND DOMAIN PPC.
RX PubMed=24218605; DOI=10.1073/pnas.1219277110;
RA Zhao J., Favero D.S., Peng H., Neff M.M.;
RT "Arabidopsis thaliana AHL family modulates hypocotyl growth redundantly by
RT interacting with each other via the PPC/DUF296 domain.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E4688-E4697(2013).
CC -!- FUNCTION: Transcription factor that specifically binds AT-rich DNA
CC sequences related to the nuclear matrix attachment regions (MARs).
CC Binds to the MARs present in the ETTIN (ETT) promoter leading to a
CC negative regulation of its gene expression. Functions as a molecular
CC node downstream of the homeotic protein AGAMOUS (AG), regulating
CC patterning and differentiation of reproductive organs. Acts as a
CC chromatin remodeling factor that modifies the architecture of ETTIN
CC (ETT) chromatin by modulating H3 methylation leading to the regulation
CC of ETT expression. Seems to be involved in the regulation of a set of
CC reproductives genes including CRABS CLAW (CRC), JAGGED (JAG) and
CC KNUCKLES (KNU). {ECO:0000269|PubMed:19956801}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:19956801}.
CC -!- TISSUE SPECIFICITY: Preferentially expressed in roots, but also in
CC flowers and leaves. Detected in the inflorescence meristem, floral
CC primordia and developing reproductive organs.
CC {ECO:0000269|PubMed:19956801}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout floral primordia at stages 1
CC through 4. At stage 6 and later, expression is confined to reproductive
CC organ primordia. At stages later than stage 10, localizes in developing
CC ovules and anther locules. {ECO:0000269|PubMed:19956801}.
CC -!- DOMAIN: The PPC domain mediates interactions between AHL proteins.
CC {ECO:0000269|PubMed:24218605}.
CC -!- DISRUPTION PHENOTYPE: Reproductive defects.
CC {ECO:0000269|PubMed:19956801}.
CC -!- MISCELLANEOUS: Overexpression of AHL21 results in reproductive defects
CC such as excessive outgrowth of stigmatic tissues, short valves, and
CC excessive proliferation of a carpelloid organ at the lateral side of a
CC pistil with exposed ovules. {ECO:0000269|PubMed:19956801}.
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DR EMBL; AC004667; AAC61811.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09088.1; -; Genomic_DNA.
DR EMBL; BR000357; FAA00292.1; -; mRNA.
DR PIR; E84766; E84766.
DR RefSeq; NP_181070.1; NM_129079.2.
DR AlphaFoldDB; O82166; -.
DR SMR; O82166; -.
DR STRING; 3702.AT2G35270.1; -.
DR PaxDb; O82166; -.
DR ProteomicsDB; 244669; -.
DR EnsemblPlants; AT2G35270.1; AT2G35270.1; AT2G35270.
DR GeneID; 818094; -.
DR Gramene; AT2G35270.1; AT2G35270.1; AT2G35270.
DR KEGG; ath:AT2G35270; -.
DR Araport; AT2G35270; -.
DR TAIR; locus:2063469; AT2G35270.
DR eggNOG; ENOG502QV4F; Eukaryota.
DR HOGENOM; CLU_039808_2_0_1; -.
DR InParanoid; O82166; -.
DR OMA; NMYSEAT; -.
DR OrthoDB; 1223871at2759; -.
DR PhylomeDB; O82166; -.
DR PRO; PR:O82166; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82166; baseline and differential.
DR Genevisible; O82166; AT.
DR GO; GO:0005654; C:nucleoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0003680; F:minor groove of adenine-thymine-rich DNA binding; IDA:UniProtKB.
DR GO; GO:0048653; P:anther development; IMP:TAIR.
DR GO; GO:0048440; P:carpel development; IMP:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEP:TAIR.
DR CDD; cd11378; DUF296; 1.
DR InterPro; IPR014476; AHL15-29.
DR InterPro; IPR005175; PPC_dom.
DR PANTHER; PTHR31100; PTHR31100; 1.
DR Pfam; PF03479; PCC; 1.
DR PROSITE; PS51742; PPC; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..285
FT /note="AT-hook motif nuclear-localized protein 21"
FT /id="PRO_0000432039"
FT DOMAIN 102..238
FT /note="PPC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01078"
FT DNA_BIND 78..90
FT /note="A.T hook"
FT /evidence="ECO:0000305"
FT REGION 17..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 285 AA; 29144 MW; B7BC11E3732CB968 CRC64;
MAGLDLGTTS RYVHNVDGGG GGQFTTDNHH EDDGGAGGNH HHHHHNHNHH QGLDLIASND
NSGLGGGGGG GSGDLVMRRP RGRPAGSKNK PKPPVIVTRE SANTLRAHIL EVGSGCDVFE
CISTYARRRQ RGICVLSGTG TVTNVSIRQP TAAGAVVTLR GTFEILSLSG SFLPPPAPPG
ATSLTIFLAG AQGQVVGGNV VGELMAAGPV MVMAASFTNV AYERLPLDEH EEHLQSGGGG
GGGNMYSEAT GGGGGLPFFN LPMSMPQIGV ESWQGNHAGA GRAPF
