ID   E_PRRSS                 Reviewed;          70 AA.
AC   A0MD31;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   07-OCT-2020, entry version 25.
DE   RecName: Full=Envelope small membrane protein;
DE            Short=Protein E;
DE   AltName: Full=Glycoprotein 2b;
DE            Short=Protein GP2b;
DE   AltName: Full=Gs;
GN   Name=GP2b; ORFNames=2b;
OS   Porcine reproductive and respiratory syndrome virus (isolate Pig/United
OS   States/SD 01-08/2001) (PRRSV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Arnidovirineae; Arteriviridae; unclassified Arteriviridae.
OX   NCBI_TaxID=857306;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Infectious clone SD 01-08;
RX   PubMed=17037606; DOI=10.1007/978-0-387-33012-9_110;
RA   Fang Y., Faaberg K.S., Rowland R.R., Christopher-Hennings J.,
RA   Pattnaik A.K., Osorio F., Nelson E.A.;
RT   "Construction of a full-length cDNA infectious clone of a European-like
RT   Type 1 PRRSV isolated in the U.S.";
RL   Adv. Exp. Med. Biol. 581:605-608(2006).
RN   [2]
RP   TOPOLOGY, AND SUBCELLULAR LOCATION.
RC   STRAIN=VR-2332;
RX   PubMed=20600392; DOI=10.1016/j.virusres.2010.06.012;
RA   Yu M., Liu X., Sun L., Chen C., Ma G., Kitamura Y., Gao G.F., Liu W.;
RT   "Subcellular localization and topology of porcine reproductive and
RT   respiratory syndrome virus E protein.";
RL   Virus Res. 152:104-114(2010).
CC   -!- FUNCTION: Minor envelope protein. May function as a viroporin in the
CC       virion envelope that facilitates uncoating of the virus in order to
CC       release the genomic RNA into the cytoplasm for subsequent replication
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homooligomer (By similarity). Associates with itself into
CC       higher-order structures, including dimers, trimers and tetramers.
CC       Associates with the GP2b-GP3-GP4 complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}; Single-pass type I
CC       membrane protein {ECO:0000250}. Host endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:20600392}; Single-pass type I membrane protein
CC       {ECO:0000269|PubMed:20600392}. Host Golgi apparatus membrane
CC       {ECO:0000269|PubMed:20600392}; Single-pass type I membrane protein
CC       {ECO:0000269|PubMed:20600392}. Secreted {ECO:0000250}.
CC   -!- PTM: Myristoylated. {ECO:0000250}.
CC   -!- PTM: Not glycosylated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the arteriviridae E protein family.
CC       {ECO:0000305}.
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DR   EMBL; DQ489311; ABF66343.1; -; Genomic_RNA.
DR   Proteomes; UP000000937; Genome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0044385; C:integral to membrane of host cell; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005216; F:ion channel activity; IEA:UniProtKB-KW.
DR   GO; GO:0039707; P:pore formation by virus in membrane of host cell; IEA:UniProtKB-KW.
DR   GO; GO:0051259; P:protein complex oligomerization; IEA:UniProtKB-KW.
DR   InterPro; IPR009775; GP2b.
DR   Pfam; PF07069; PRRSV_2b; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein; Host endoplasmic reticulum; Host Golgi apparatus;
KW   Host membrane; Ion channel; Ion transport; Lipoprotein; Membrane;
KW   Myristate; Secreted; Transmembrane; Transmembrane helix; Transport;
KW   Viral envelope protein; Viral ion channel; Virion.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000255"
FT   CHAIN           2..70
FT                   /note="Envelope small membrane protein"
FT                   /id="PRO_0000410886"
FT   TOPO_DOM        2..25
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000305|PubMed:20600392"
FT   TRANSMEM        26..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        47..70
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000305|PubMed:20600392"
FT   REGION          2..15
FT                   /note="Endoplasmic reticulum retention signal"
FT                   /evidence="ECO:0000255"
FT   LIPID           2
FT                   /note="N-myristoyl glycine; by host"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   70 AA;  7771 MW;  02090C77E7FD4753 CRC64;
     MGSLWSKISQ LFVDAFTEFL VSVVDIVIFL AILFGFTVAG WLLVFLLRVV CSALLRSRSA
     IHSPELSKVL