AHL25_ARATH
ID AHL25_ARATH Reviewed; 299 AA.
AC Q6DBQ1; O65489;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=AT-hook motif nuclear-localized protein 25 {ECO:0000312|EMBL:FAA00296.1};
DE AltName: Full=AT-hook protein of GA feedback 1 {ECO:0000303|PubMed:17277098};
GN Name=AHL25 {ECO:0000303|PubMed:15604740};
GN Synonyms=AGF1 {ECO:0000303|PubMed:17277098}, HRC;
GN OrderedLocusNames=At4g35390 {ECO:0000312|Araport:AT4G35390};
GN ORFNames=F23E12.50 {ECO:0000312|EMBL:CAA18730.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=15604740; DOI=10.1007/s11103-004-3249-5;
RA Fujimoto S., Matsunaga S., Yonemura M., Uchiyama S., Azuma T., Fukui K.;
RT "Identification of a novel plant MAR DNA binding protein localized on
RT chromosomal surfaces.";
RL Plant Mol. Biol. 56:225-239(2004).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=17277098; DOI=10.1104/pp.106.093542;
RA Matsushita A., Furumoto T., Ishida S., Takahashi Y.;
RT "AGF1, an AT-hook protein, is necessary for the negative feedback of
RT AtGA3ox1 encoding GA 3-oxidase.";
RL Plant Physiol. 143:1152-1162(2007).
RN [6]
RP GENE FAMILY, SUBUNIT, INTERACTION WITH AHL27 AND AHL29, AND DOMAIN PPC.
RX PubMed=24218605; DOI=10.1073/pnas.1219277110;
RA Zhao J., Favero D.S., Peng H., Neff M.M.;
RT "Arabidopsis thaliana AHL family modulates hypocotyl growth redundantly by
RT interacting with each other via the PPC/DUF296 domain.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E4688-E4697(2013).
CC -!- FUNCTION: Transcription factor that specifically binds AT-rich DNA
CC sequences related to the nuclear matrix attachment regions (MARs) (By
CC similarity). Binds the DNA sequence GNFEI (GA-negative feedback element
CC I) in the GA3OX1 promoter. Binding to GNFEI sequence is required for
CC GA-negative feedback regulation of GA3OX1.
CC {ECO:0000250|UniProtKB:Q8VYJ2, ECO:0000269|PubMed:17277098}.
CC -!- SUBUNIT: Homodimer. Interacts with AHL27 and AHL29.
CC {ECO:0000269|PubMed:24218605}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17277098}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, leaves, stems, floral tips
CC and flowers. {ECO:0000269|PubMed:17277098}.
CC -!- DOMAIN: The PPC domain mediates interactions between AHL proteins.
CC {ECO:0000269|PubMed:24218605}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA18730.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB80256.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL022604; CAA18730.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL161587; CAB80256.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002687; AEE86506.1; -; Genomic_DNA.
DR EMBL; BT014971; AAT70422.1; -; mRNA.
DR EMBL; BT015845; AAU94408.1; -; mRNA.
DR EMBL; BR000361; FAA00296.1; -; mRNA.
DR PIR; T06118; T06118.
DR RefSeq; NP_195265.2; NM_119705.3.
DR AlphaFoldDB; Q6DBQ1; -.
DR SMR; Q6DBQ1; -.
DR STRING; 3702.AT4G35390.1; -.
DR iPTMnet; Q6DBQ1; -.
DR PaxDb; Q6DBQ1; -.
DR PRIDE; Q6DBQ1; -.
DR ProteomicsDB; 244851; -.
DR EnsemblPlants; AT4G35390.1; AT4G35390.1; AT4G35390.
DR GeneID; 829692; -.
DR Gramene; AT4G35390.1; AT4G35390.1; AT4G35390.
DR KEGG; ath:AT4G35390; -.
DR Araport; AT4G35390; -.
DR TAIR; locus:2122148; AT4G35390.
DR eggNOG; ENOG502QV94; Eukaryota.
DR HOGENOM; CLU_039808_2_0_1; -.
DR InParanoid; Q6DBQ1; -.
DR OMA; SGAQACE; -.
DR OrthoDB; 1455505at2759; -.
DR PhylomeDB; Q6DBQ1; -.
DR PRO; PR:Q6DBQ1; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q6DBQ1; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0003680; F:minor groove of adenine-thymine-rich DNA binding; IBA:GO_Central.
DR GO; GO:0043621; F:protein self-association; IDA:UniProtKB.
DR GO; GO:0009938; P:negative regulation of gibberellic acid mediated signaling pathway; IEP:TAIR.
DR GO; GO:0010228; P:vegetative to reproductive phase transition of meristem; IBA:GO_Central.
DR CDD; cd11378; DUF296; 1.
DR InterPro; IPR014476; AHL15-29.
DR InterPro; IPR005175; PPC_dom.
DR PANTHER; PTHR31100; PTHR31100; 1.
DR Pfam; PF03479; PCC; 1.
DR PIRSF; PIRSF016021; ESCAROLA; 1.
DR PROSITE; PS51742; PPC; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..299
FT /note="AT-hook motif nuclear-localized protein 25"
FT /id="PRO_0000432043"
FT DOMAIN 87..233
FT /note="PPC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01078"
FT DNA_BIND 63..75
FT /note="A.T hook"
FT /evidence="ECO:0000305"
FT REGION 1..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 216..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 45..62
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..251
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 299 AA; 30148 MW; 1C07A72FBB75AAC6 CRC64;
MSSYMHPLLG QELHLQRPED SRTPPDQNNM ELNRSEADEA KAETTPTGGA TSSATASGSS
SGRRPRGRPA GSKNKPKPPT IITRDSPNVL RSHVLEVTSG SDISEAVSTY ATRRGCGVCI
ISGTGAVTNV TIRQPAAPAG GGVITLHGRF DILSLTGTAL PPPAPPGAGG LTVYLAGGQG
QVVGGNVAGS LIASGPVVLM AASFANAVYD RLPIEEEETP PPRTTGVQQQ QPEASQSSEV
TGSGAQACES NLQGGNGGGG VAFYNLGMNM NNFQFSGGDI YGMSGGSGGG GGGATRPAF
