AHL27_ARATH
ID AHL27_ARATH Reviewed; 311 AA.
AC Q9S7C9; Q0WRK9;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=AT-hook motif nuclear-localized protein 27 {ECO:0000312|EMBL:FAA00298.1};
DE AltName: Full=DNA-binding protein ESCAROLA {ECO:0000303|PubMed:10759496};
DE AltName: Full=Protein ORESARA 7 {ECO:0000303|PubMed:17971039};
GN Name=AHL27 {ECO:0000303|PubMed:15604740};
GN Synonyms=ESC {ECO:0000312|EMBL:AAF07197.1},
GN ORE7 {ECO:0000303|PubMed:17971039};
GN OrderedLocusNames=At1g20900 {ECO:0000312|Araport:AT1G20900};
GN ORFNames=F9H16.12 {ECO:0000312|EMBL:AAD30602.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=10759496; DOI=10.1104/pp.122.4.1003;
RA Weigel D., Ahn J.H., Blazquez M.A., Borevitz J.O., Christensen S.K.,
RA Fankhauser C., Ferrandiz C., Kardailsky I., Malancharuvil E.J., Neff M.M.,
RA Nguyen J.T., Sato S., Wang Z.Y., Xia Y., Dixon R.A., Harrison M.J.,
RA Lamb C.J., Yanofsky M.F., Chory J.;
RT "Activation tagging in Arabidopsis.";
RL Plant Physiol. 122:1003-1013(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP IDENTIFICATION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=15604740; DOI=10.1007/s11103-004-3249-5;
RA Fujimoto S., Matsunaga S., Yonemura M., Uchiyama S., Azuma T., Fukui K.;
RT "Identification of a novel plant MAR DNA binding protein localized on
RT chromosomal surfaces.";
RL Plant Mol. Biol. 56:225-239(2004).
RN [8]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17971039; DOI=10.1111/j.1365-313x.2007.03317.x;
RA Lim P.O., Kim Y., Breeze E., Koo J.C., Woo H.R., Ryu J.S., Park D.H.,
RA Beynon J., Tabrett A., Buchanan-Wollaston V., Nam H.G.;
RT "Overexpression of a chromatin architecture-controlling AT-hook protein
RT extends leaf longevity and increases the post-harvest storage life of
RT plants.";
RL Plant J. 52:1140-1153(2007).
RN [9]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18088311; DOI=10.1111/j.1365-313x.2007.03393.x;
RA Street I.H., Shah P.K., Smith A.M., Avery N., Neff M.M.;
RT "The AT-hook-containing proteins SOB3/AHL29 and ESC/AHL27 are negative
RT modulators of hypocotyl growth in Arabidopsis.";
RL Plant J. 54:1-14(2008).
RN [10]
RP FUNCTION.
RX PubMed=19517252; DOI=10.1007/s11103-009-9507-9;
RA Xiao C., Chen F., Yu X., Lin C., Fu Y.F.;
RT "Over-expression of an AT-hook gene, AHL22, delays flowering and inhibits
RT the elongation of the hypocotyl in Arabidopsis thaliana.";
RL Plant Mol. Biol. 71:39-50(2009).
RN [11]
RP FUNCTION.
RX PubMed=20738724; DOI=10.1111/j.1744-7909.2010.00969.x;
RA Lu H., Zou Y., Feng N.;
RT "Overexpression of AHL20 negatively regulates defenses in Arabidopsis.";
RL J. Integr. Plant Biol. 52:801-808(2010).
RN [12]
RP GENE FAMILY, MUTAGENESIS OF ARG-91, SUBUNIT, INTERACTION WITH AHL12; AHL25;
RP AHL29; TCP4; TCP13; EF114; ATAF2/NAC081; H2B.1; H3.3 AND H4, AND DOMAIN
RP PPC.
RX PubMed=24218605; DOI=10.1073/pnas.1219277110;
RA Zhao J., Favero D.S., Peng H., Neff M.M.;
RT "Arabidopsis thaliana AHL family modulates hypocotyl growth redundantly by
RT interacting with each other via the PPC/DUF296 domain.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E4688-E4697(2013).
CC -!- FUNCTION: Transcription factor that specifically binds AT-rich DNA
CC sequences related to the nuclear matrix attachment regions (MARs)
CC (PubMed:17971039, PubMed:19517252). Negatively regulates plant innate
CC immunity (PTI) to pathogens through the down-regulation of the PAMP-
CC triggered FRK1 expression (PubMed:20738724). Acts redundantly with
CC AHL18, AHL22 and AHL29 in the regulation of flowering and regulation of
CC the hypocotyl elongation (PubMed:19517252). Acts as a chromatin
CC remodeling factor that negatively regulates the leaf senescence
CC (PubMed:17971039). Acts redundantly with AHL29/SOB3 to modulate
CC hypocotyl growth inhibition in response to light (PubMed:18088311).
CC {ECO:0000269|PubMed:17971039, ECO:0000269|PubMed:18088311,
CC ECO:0000269|PubMed:19517252, ECO:0000269|PubMed:20738724}.
CC -!- SUBUNIT: Homodimer. Interacts with AHL12, AHL25, AHL29, TCP4, TCP13,
CC EF114, ATAF2/NAC081, histone H2B.1, histone H3.3 and histone H4.
CC {ECO:0000269|PubMed:24218605}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17971039,
CC ECO:0000269|PubMed:18088311}.
CC -!- TISSUE SPECIFICITY: Expressed in the hypocotyl and the vascular tissue
CC of seedling. {ECO:0000269|PubMed:18088311}.
CC -!- DOMAIN: The PPC domain mediates interactions between AHL proteins.
CC {ECO:0000269|PubMed:24218605}.
CC -!- DISRUPTION PHENOTYPE: AHL27 and AHL29 double mutant exhibit a long
CC hypocotyl phenotype in the light. {ECO:0000269|PubMed:18088311}.
CC -!- MISCELLANEOUS: Overexpression of AHL27 results in a decreased flg22-
CC induced expression of FRK1 (PubMed:20738724). Overexpression causes
CC also delay of leaf senescence, late flowering and modified leaf
CC development (PubMed:10759496, PubMed:17971039).
CC {ECO:0000269|PubMed:10759496, ECO:0000269|PubMed:17971039,
CC ECO:0000269|PubMed:20738724}.
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DR EMBL; AF194974; AAF07197.1; -; mRNA.
DR EMBL; AC007369; AAD30602.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30038.1; -; Genomic_DNA.
DR EMBL; BT006460; AAP21268.1; -; mRNA.
DR EMBL; AK228296; BAF00240.1; -; mRNA.
DR EMBL; AB493469; BAH30307.1; -; mRNA.
DR EMBL; BR000363; FAA00298.1; -; mRNA.
DR PIR; F86341; F86341.
DR RefSeq; NP_173514.1; NM_101943.2.
DR AlphaFoldDB; Q9S7C9; -.
DR SMR; Q9S7C9; -.
DR BioGRID; 23922; 17.
DR STRING; 3702.AT1G20900.1; -.
DR PaxDb; Q9S7C9; -.
DR PRIDE; Q9S7C9; -.
DR ProteomicsDB; 244845; -.
DR EnsemblPlants; AT1G20900.1; AT1G20900.1; AT1G20900.
DR GeneID; 838683; -.
DR Gramene; AT1G20900.1; AT1G20900.1; AT1G20900.
DR KEGG; ath:AT1G20900; -.
DR Araport; AT1G20900; -.
DR TAIR; locus:2037350; AT1G20900.
DR eggNOG; ENOG502QV94; Eukaryota.
DR HOGENOM; CLU_039808_2_2_1; -.
DR InParanoid; Q9S7C9; -.
DR OMA; QMQHAPS; -.
DR OrthoDB; 1350381at2759; -.
DR PhylomeDB; Q9S7C9; -.
DR PRO; PR:Q9S7C9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9S7C9; baseline and differential.
DR Genevisible; Q9S7C9; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:TAIR.
DR GO; GO:0042393; F:histone binding; IPI:UniProtKB.
DR GO; GO:0003680; F:minor groove of adenine-thymine-rich DNA binding; IDA:TAIR.
DR GO; GO:0043621; F:protein self-association; IDA:UniProtKB.
DR GO; GO:0006325; P:chromatin organization; IMP:TAIR.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0010150; P:leaf senescence; IMP:TAIR.
DR GO; GO:0045824; P:negative regulation of innate immune response; IMP:UniProtKB.
DR GO; GO:0009640; P:photomorphogenesis; IMP:TAIR.
DR GO; GO:0010228; P:vegetative to reproductive phase transition of meristem; IMP:UniProtKB.
DR CDD; cd11378; DUF296; 1.
DR InterPro; IPR014476; AHL15-29.
DR InterPro; IPR005175; PPC_dom.
DR PANTHER; PTHR31100; PTHR31100; 1.
DR Pfam; PF03479; PCC; 1.
DR PIRSF; PIRSF016021; ESCAROLA; 1.
DR PROSITE; PS51742; PPC; 1.
PE 1: Evidence at protein level;
KW Apoptosis; DNA-binding; Flowering; Immunity; Innate immunity; Nucleus;
KW Plant defense; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..311
FT /note="AT-hook motif nuclear-localized protein 27"
FT /id="PRO_0000087056"
FT DOMAIN 110..258
FT /note="PPC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01078"
FT DNA_BIND 86..98
FT /note="A.T hook"
FT /evidence="ECO:0000255"
FT REGION 40..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 178..183
FT /note="Required for the binding to non-AHL interactors"
FT /evidence="ECO:0000269|PubMed:24218605"
FT REGION 246..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 54..73
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 91
FT /note="R->H: In esc-11; Exhibits a long hypocotyl phenotype
FT in the light."
FT /evidence="ECO:0000269|PubMed:24218605"
SQ SEQUENCE 311 AA; 31842 MW; A80B445C9776EB7D CRC64;
MEGGYEQGGG ASRYFHNLFR PEIHHQQLQP QGGINLIDQH HHQHQQHQQQ QQPSDDSRES
DHSNKDHHQQ GRPDSDPNTS SSAPGKRPRG RPPGSKNKAK PPIIVTRDSP NALRSHVLEV
SPGADIVESV STYARRRGRG VSVLGGNGTV SNVTLRQPVT PGNGGGVSGG GGVVTLHGRF
EILSLTGTVL PPPAPPGAGG LSIFLAGGQG QVVGGSVVAP LIASAPVILM AASFSNAVFE
RLPIEEEEEE GGGGGGGGGG GPPQMQQAPS ASPPSGVTGQ GQLGGNVGGY GFSGDPHLLG
WGAGTPSRPP F
