AHL3_ARATH
ID AHL3_ARATH Reviewed; 404 AA.
AC Q9SB31;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=AT-hook motif nuclear-localized protein 3 {ECO:0000312|EMBL:FAA00274.1};
GN Name=AHL3 {ECO:0000303|PubMed:15604740};
GN OrderedLocusNames=At4g25320 {ECO:0000312|Araport:AT4G25320};
GN ORFNames=F24A6.160 {ECO:0000312|EMBL:CAA23073.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=15604740; DOI=10.1007/s11103-004-3249-5;
RA Fujimoto S., Matsunaga S., Yonemura M., Uchiyama S., Azuma T., Fukui K.;
RT "Identification of a novel plant MAR DNA binding protein localized on
RT chromosomal surfaces.";
RL Plant Mol. Biol. 56:225-239(2004).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH AHL4, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23335615; DOI=10.1105/tpc.112.102210;
RA Zhou J., Wang X., Lee J.Y., Lee J.Y.;
RT "Cell-to-cell movement of two interacting AT-hook factors in Arabidopsis
RT root vascular tissue patterning.";
RL Plant Cell 25:187-201(2013).
RN [6]
RP GENE FAMILY, AND DOMAIN PPC.
RX PubMed=24218605; DOI=10.1073/pnas.1219277110;
RA Zhao J., Favero D.S., Peng H., Neff M.M.;
RT "Arabidopsis thaliana AHL family modulates hypocotyl growth redundantly by
RT interacting with each other via the PPC/DUF296 domain.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E4688-E4697(2013).
CC -!- FUNCTION: Transcription factor that specifically binds AT-rich DNA
CC sequences related to the nuclear matrix attachment regions (MARs) (By
CC similarity). Acts redundantly with AHL4 to regulate the formation of
CC tissue boundary between the xylem and procambium in the root meristem
CC (PubMed:23335615). {ECO:0000250|UniProtKB:Q8VYJ2,
CC ECO:0000269|PubMed:23335615}.
CC -!- SUBUNIT: Homodimer. Interacts with AHL4. {ECO:0000269|PubMed:23335615}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23335615}.
CC -!- TISSUE SPECIFICITY: Expressed in both procambium and xylem precursors
CC of the root meristem. Also detected in the endodermis in the late
CC elongation zone and onwards. {ECO:0000269|PubMed:23335615}.
CC -!- DOMAIN: The PPC domain mediates interactions between AHL proteins.
CC {ECO:0000269|PubMed:24218605}.
CC -!- DISRUPTION PHENOTYPE: Misspecification of tissue boundaries between the
CC xylem and procambium. {ECO:0000269|PubMed:23335615}.
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DR EMBL; AL035396; CAA23073.1; -; Genomic_DNA.
DR EMBL; AL161563; CAB81343.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85041.1; -; Genomic_DNA.
DR EMBL; AY099572; AAM20424.1; -; mRNA.
DR EMBL; BT003408; AAO30071.1; -; mRNA.
DR EMBL; BR000339; FAA00274.1; -; mRNA.
DR PIR; T05553; T05553.
DR RefSeq; NP_194262.1; NM_118664.4.
DR AlphaFoldDB; Q9SB31; -.
DR SMR; Q9SB31; -.
DR IntAct; Q9SB31; 2.
DR STRING; 3702.AT4G25320.1; -.
DR PaxDb; Q9SB31; -.
DR PRIDE; Q9SB31; -.
DR ProteomicsDB; 244801; -.
DR EnsemblPlants; AT4G25320.1; AT4G25320.1; AT4G25320.
DR GeneID; 828635; -.
DR Gramene; AT4G25320.1; AT4G25320.1; AT4G25320.
DR KEGG; ath:AT4G25320; -.
DR Araport; AT4G25320; -.
DR TAIR; locus:2122684; AT4G25320.
DR eggNOG; ENOG502QTAR; Eukaryota.
DR HOGENOM; CLU_039808_0_0_1; -.
DR InParanoid; Q9SB31; -.
DR OMA; HENTANA; -.
DR OrthoDB; 1053982at2759; -.
DR PhylomeDB; Q9SB31; -.
DR PRO; PR:Q9SB31; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SB31; baseline and differential.
DR Genevisible; Q9SB31; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003680; F:minor groove of adenine-thymine-rich DNA binding; IEA:InterPro.
DR GO; GO:0043621; F:protein self-association; IDA:UniProtKB.
DR GO; GO:0010051; P:xylem and phloem pattern formation; IMP:UniProtKB.
DR GO; GO:0010089; P:xylem development; IMP:TAIR.
DR CDD; cd11378; DUF296; 1.
DR InterPro; IPR039605; AHL.
DR InterPro; IPR005175; PPC_dom.
DR PANTHER; PTHR31500; PTHR31500; 1.
DR Pfam; PF03479; PCC; 1.
DR PROSITE; PS51742; PPC; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..404
FT /note="AT-hook motif nuclear-localized protein 3"
FT /id="PRO_0000432021"
FT DOMAIN 163..308
FT /note="PPC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01078"
FT DNA_BIND 86..98
FT /note="A.T hook"
FT /evidence="ECO:0000255"
FT REGION 1..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 70..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 113..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 359..404
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 86..94
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000305"
FT COMPBIAS 7..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..50
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 373..396
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 404 AA; 43014 MW; FD0E81CDA7740F01 CRC64;
MEEREGTNIN NNITSSFGLK QQHEAAASDG GYSMDPPPRP ENPNPFLVPP TTVPAAATVA
AAVTENAATP FSLTMPTENT SAEQLKKKRG RPRKYNPDGT LVVTLSPMPI SSSVPLTSEF
PPRKRGRGRG KSNRWLKKSQ MFQFDRSPVD TNLAGVGTAD FVGANFTPHV LIVNAGEDVT
MKIMTFSQQG SRAICILSAN GPISNVTLRQ SMTSGGTLTY EGRFEILSLT GSFMQNDSGG
TRSRAGGMSV CLAGPDGRVF GGGLAGLFLA AGPVQVMVGT FIAGQEQSQL ELAKERRLRF
GAQPSSISFN ISAEERKARF ERLNKSVAIP APTTSYTHVN TTNAVHSYYT NSVNHVKDPF
SSIPVGGGGG GEVGEEEGEE DDDELEGEDE EFGGDSQSDN EIPS
