F120B_HUMAN
ID F120B_HUMAN Reviewed; 910 AA.
AC Q96EK7; B4DL34; Q86V68; Q96JI9;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Constitutive coactivator of peroxisome proliferator-activated receptor gamma;
DE Short=Constitutive coactivator of PPAR-gamma;
DE Short=Constitutive coactivator of PPARG;
DE AltName: Full=PPARG constitutive coactivator 1;
DE Short=PGCC1;
DE AltName: Full=Protein FAM120B;
GN Name=FAM120B; Synonyms=CCPG, KIAA1838;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=17595322; DOI=10.1210/me.2006-0520;
RA Li D., Kang Q., Wang D.-M.;
RT "Constitutive coactivator of peroxisome proliferator-activated receptor
RT (PPARgamma), a novel coactivator of PPARgamma that promotes adipogenesis.";
RL Mol. Endocrinol. 21:2320-2333(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=11347906; DOI=10.1093/dnares/8.2.85;
RA Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XX. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 8:85-95(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 705-910 (ISOFORM 2), AND TISSUE SPECIFICITY.
RX PubMed=14585507; DOI=10.1016/s0378-1119(03)00770-4;
RA Holden S., Raymond F.L.;
RT "The human gene CXorf17 encodes a member of a novel family of putative
RT transmembrane proteins: cDNA cloning and characterization of CXorf17 and
RT its mouse ortholog orf34.";
RL Gene 318:149-161(2003).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-885, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Colon carcinoma;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- FUNCTION: Functions as a transactivator of PPARG and ESR1. Functions in
CC adipogenesis through PPARG activation (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with ESR1 and RXRA. Interacts with PPARG; in a
CC ligand-independent manner (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q96EK7; Q12933: TRAF2; NbExp=4; IntAct=EBI-739883, EBI-355744;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q96EK7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96EK7-2; Sequence=VSP_033415, VSP_033416;
CC Name=3;
CC IsoId=Q96EK7-3; Sequence=VSP_055267;
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:14585507}.
CC -!- SIMILARITY: Belongs to the constitutive coactivator of PPAR-gamma
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB47467.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; DQ873695; ABH09086.1; -; mRNA.
DR EMBL; AB058741; BAB47467.1; ALT_INIT; mRNA.
DR EMBL; AK296825; BAG59396.1; -; mRNA.
DR EMBL; AL008628; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL078605; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471051; EAW47424.1; -; Genomic_DNA.
DR EMBL; BC012177; AAH12177.1; -; mRNA.
DR EMBL; AY266458; AAP31032.1; -; mRNA.
DR CCDS; CCDS5314.1; -. [Q96EK7-1]
DR CCDS; CCDS69243.1; -. [Q96EK7-3]
DR RefSeq; NP_001273310.1; NM_001286381.1. [Q96EK7-3]
DR RefSeq; NP_115824.1; NM_032448.2. [Q96EK7-1]
DR AlphaFoldDB; Q96EK7; -.
DR BioGRID; 124101; 31.
DR IntAct; Q96EK7; 13.
DR STRING; 9606.ENSP00000440125; -.
DR iPTMnet; Q96EK7; -.
DR MetOSite; Q96EK7; -.
DR PhosphoSitePlus; Q96EK7; -.
DR BioMuta; FAM120B; -.
DR DMDM; 74751843; -.
DR EPD; Q96EK7; -.
DR jPOST; Q96EK7; -.
DR MassIVE; Q96EK7; -.
DR MaxQB; Q96EK7; -.
DR PaxDb; Q96EK7; -.
DR PeptideAtlas; Q96EK7; -.
DR PRIDE; Q96EK7; -.
DR ProteomicsDB; 4505; -.
DR ProteomicsDB; 76419; -. [Q96EK7-1]
DR ProteomicsDB; 76420; -. [Q96EK7-2]
DR Antibodypedia; 33580; 91 antibodies from 23 providers.
DR DNASU; 84498; -.
DR Ensembl; ENST00000476287.4; ENSP00000417970.1; ENSG00000112584.14. [Q96EK7-1]
DR Ensembl; ENST00000616072.2; ENSP00000482544.2; ENSG00000275333.4. [Q96EK7-3]
DR Ensembl; ENST00000617082.4; ENSP00000481344.1; ENSG00000275333.4. [Q96EK7-1]
DR Ensembl; ENST00000625626.1; ENSP00000485793.1; ENSG00000112584.14. [Q96EK7-3]
DR GeneID; 84498; -.
DR KEGG; hsa:84498; -.
DR MANE-Select; ENST00000476287.4; ENSP00000417970.1; NM_032448.3; NP_115824.1.
DR UCSC; uc003qxp.5; human. [Q96EK7-1]
DR CTD; 84498; -.
DR DisGeNET; 84498; -.
DR GeneCards; FAM120B; -.
DR HGNC; HGNC:21109; FAM120B.
DR HPA; ENSG00000112584; Low tissue specificity.
DR MIM; 612266; gene.
DR neXtProt; NX_Q96EK7; -.
DR OpenTargets; ENSG00000112584; -.
DR PharmGKB; PA134920525; -.
DR VEuPathDB; HostDB:ENSG00000112584; -.
DR eggNOG; ENOG502QRMW; Eukaryota.
DR GeneTree; ENSGT00530000063168; -.
DR HOGENOM; CLU_007639_0_0_1; -.
DR InParanoid; Q96EK7; -.
DR PhylomeDB; Q96EK7; -.
DR TreeFam; TF331093; -.
DR PathwayCommons; Q96EK7; -.
DR Reactome; R-HSA-1989781; PPARA activates gene expression.
DR Reactome; R-HSA-381340; Transcriptional regulation of white adipocyte differentiation.
DR SignaLink; Q96EK7; -.
DR BioGRID-ORCS; 84498; 15 hits in 1086 CRISPR screens.
DR ChiTaRS; FAM120B; human.
DR GenomeRNAi; 84498; -.
DR Pharos; Q96EK7; Tbio.
DR PRO; PR:Q96EK7; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q96EK7; protein.
DR Bgee; ENSG00000112584; Expressed in right uterine tube and 94 other tissues.
DR ExpressionAtlas; Q96EK7; baseline and differential.
DR Genevisible; Q96EK7; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0045444; P:fat cell differentiation; IBA:GO_Central.
DR GO; GO:0035357; P:peroxisome proliferator activated receptor signaling pathway; IBA:GO_Central.
DR InterPro; IPR026784; Coact_PPARg.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR PANTHER; PTHR15976; PTHR15976; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Differentiation; Methylation; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..910
FT /note="Constitutive coactivator of peroxisome proliferator-
FT activated receptor gamma"
FT /id="PRO_0000332990"
FT REGION 333..416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 443..483
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 863..910
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 874..895
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 885
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT VAR_SEQ 1..668
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055267"
FT VAR_SEQ 762..764
FT /note="PDY -> VQT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14585507"
FT /id="VSP_033415"
FT VAR_SEQ 765..910
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14585507"
FT /id="VSP_033416"
FT VARIANT 370
FT /note="D -> Y (in dbSNP:rs6917485)"
FT /id="VAR_043021"
FT VARIANT 379
FT /note="M -> T (in dbSNP:rs6905356)"
FT /id="VAR_043022"
FT VARIANT 428
FT /note="Y -> C (in dbSNP:rs6900199)"
FT /id="VAR_043023"
FT VARIANT 430
FT /note="D -> G (in dbSNP:rs6900202)"
FT /id="VAR_043024"
FT VARIANT 431
FT /note="S -> P (in dbSNP:rs6905610)"
FT /id="VAR_043025"
FT VARIANT 433
FT /note="P -> A (in dbSNP:rs6934830)"
FT /id="VAR_043026"
FT VARIANT 440
FT /note="Y -> C (in dbSNP:rs9366138)"
FT /id="VAR_043027"
FT VARIANT 511
FT /note="C -> G (in dbSNP:rs9348266)"
FT /id="VAR_043028"
SQ SEQUENCE 910 AA; 103783 MW; 079F9385733B5FAC CRC64;
MGVRGLQGFV GSTCPHICTV VNFKELAEHH RSKYPGCTPT IVVDAMCCLR YWYTPESWIC
GGQWREYFSA LRDFVKTFTA AGIKLIFFFD GMVEQDKRDE WVKRRLKNNR EISRIFHYIK
SHKEQPGRNM FFIPSGLAVF TRFALKTLGQ ETLCSLQEAD YEVASYGLQH NCLGILGEDT
DYLIYDTCPY FSISELCLES LDTVMLCREK LCESLGLCVA DLPLLACLLG NDIIPEGMFE
SFRYKCLSSY TSVKENFDKK GNIILAVSDH ISKVLYLYQG EKKLEEILPL GPNKALFYKG
MASYLLPGQK SPWFFQKPKG VITLDKQVIS TSSDAESREE VPMCSDAESR QEVPMCTGPE
SRREVPVYTD SEPRQEVPMC SDPEPRQEVP TCTGPESRRE VPMCSDPEPR QEVPMCTGPE
ARQEVPMYTD SEPRQEVPMY TDSEPRQEVP MYTGSEPRQE VPMYTGPESR QEVPMYTGPE
SRQEVLIRTD PESRQEIMCT GHESKQEVPI CTDPISKQED SMCTHAEINQ KLPVATDFEF
KLEALMCTNP EIKQEDPTNV GPEVKQQVTM VSDTEILKVA RTHHVQAESY LVYNIMSSGE
IECSNTLEDE LDQALPSQAF IYRPIRQRVY SLLLEDCQDV TSTCLAVKEW FVYPGNPLRH
PDLVRPLQMT IPGGTPSLKI LWLNQEPEIQ VRRLDTLLAC FNLSSSREEL QAVESPFQAL
CCLLIYLFVQ VDTLCLEDLH AFIAQALCLQ GKSTSQLVNL QPDYINPRAV QLGSLLVRGL
TTLVLVNSAC GFPWKTSDFM PWNVFDGKLF HQKYLQSEKG YAVEVLLEQN RSRLTKFHNL
KAVVCKACMK ENRRITGRAH WGSHHAGRWG RQGSSYHRTG SGYSRSSQGQ PWRDQGPGSR
QYEHDQWRRY
