ID   F12_VACCA               Reviewed;         635 AA.
AC   O57178;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   29-SEP-2021, entry version 65.
DE   RecName: Full=Protein F12;
GN   OrderedLocusNames=MVA042L, ACAM3000_MVA_042; ORFNames=F12L;
OS   Vaccinia virus (strain Ankara) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=126794;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9601507; DOI=10.1006/viro.1998.9123;
RA   Antoine G., Scheiflinger F., Dorner F., Falkner F.G.;
RT   "The complete genomic sequence of the modified vaccinia Ankara strain:
RT   comparison with other orthopoxviruses.";
RL   Virology 244:365-396(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Acambis 3000;
RA   Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J.,
RA   Khristova M., Wohlhueter R.M.;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Late protein that plays a role in intracellular enveloped
CC       virus (IEV) transport to the cell surface on microtubules. When absent,
CC       viral morphogenesis halts after the formation of IEV particles, and
CC       these are not transported to the cell periphery. Important for plaque
CC       formation, extracellular envelope virus (EEV) production and virulence
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}. Host endosome
CC       {ECO:0000250}. Note=Associates with the membrane of IEV particles, but
CC       not intracellular mature virus (IMV), cell-associated enveloped virus
CC       (CEV) or EEV. Colocalizes with microtubules (By similarity).
CC       {ECO:0000250}.
CC   -!- PTM: Not glycosylated or contains very little carbohydrate.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae F12 protein family.
CC       {ECO:0000305}.
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DR   EMBL; U94848; AAB96421.1; -; Genomic_DNA.
DR   EMBL; AY603355; AAT10440.1; -; Genomic_DNA.
DR   PIR; T30788; T30788.
DR   Proteomes; UP000159908; Genome.
DR   Proteomes; UP000172909; Genome.
DR   GO; GO:0044174; C:host cell endosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016032; P:viral process; IEA:InterPro.
DR   InterPro; IPR005005; Poxvirus_F12L.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   Pfam; PF03337; Pox_F12L; 1.
DR   PIRSF; PIRSF015793; VAC_EEV; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   3: Inferred from homology;
KW   Host endosome; Late protein; Membrane; Virion; Virulence.
FT   CHAIN           1..635
FT                   /note="Protein F12"
FT                   /id="PRO_0000343639"
SQ   SEQUENCE   635 AA;  73129 MW;  7CFB37C6104B34CA CRC64;
     MLNRIQTLMK TANNYETIEI LRNYLRLYII LARNEEGHGI LIYDDNIDSV MSMMNITILE
     VIGLTTHCTK LRSSPPIPMS RLFMDEIDHE SYYSPKTSDY PLIDIIRKRS HEQGDIALAL
     ERYGIENTDS ISEINEWLSS KGLACYRFVK FNDYRKQMYR KFSRCTIVDS MIIGHIGHHY
     IWIKNLETYT RPEIDVLPFD IKYISRDELW ARISSSLDQT HIKTIAVSVY GAITDNGPIP
     YMISTYPGNT FVNFNSVKNL ILNFLDWIKD IMTSTRTIIL VGYMSNLFDI PLLTVYWPNN
     CGWKIYNNTL ISSDGARVIW MDAYKFSCGL SLQDYCYHWG SKPESRPFDL IKKSDAKRNS
     KSLVKESMAS LKSLYEAFET QSGALEVLMS PCRMFSFSRI EDMFLTSVIN RVSENTGMGM
     YYPTNDIPSL FIESSICLDY IIVNNQESNK YRIKSVLDII SSKQYPAGRP NYVKNGTKGK
     LYIALCKVTV PTNDHIPVVY HDDDNTTTFI TVLTSVDIET AIRAGYSIVE LGALQWDNNI
     PELKNGLLDS IKMIYDLNAV TTNNLLEQLI ENINFNNSSI ISLFYTFAIS YCRAFIYSIM
     ETIDPVYISQ FSYKELYVSS SYKDINESMS QMVKL