ID   F12_VACCC               Reviewed;         635 AA.
AC   P21053;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   29-SEP-2021, entry version 80.
DE   RecName: Full=Protein F12;
GN   ORFNames=F12L;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Late protein that plays a role in intracellular enveloped
CC       virus (IEV) transport to the cell surface on microtubules. When absent,
CC       viral morphogenesis halts after the formation of IEV particles, and
CC       these are not transported to the cell periphery. Important for plaque
CC       formation, extracellular envelope virus (EEV) production and virulence
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}. Host endosome
CC       {ECO:0000250}. Note=Associates with the membrane of IEV particles, but
CC       not intracellular mature virus (IMV), cell-associated enveloped virus
CC       (CEV) or EEV. Colocalizes with microtubules (By similarity).
CC       {ECO:0000250}.
CC   -!- PTM: Not glycosylated or contains very little carbohydrate.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae F12 protein family.
CC       {ECO:0000305}.
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DR   EMBL; M35027; AAA48029.1; -; Genomic_DNA.
DR   PIR; H42507; H42507.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0044174; C:host cell endosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016032; P:viral process; IEA:InterPro.
DR   InterPro; IPR005005; Poxvirus_F12L.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   Pfam; PF03337; Pox_F12L; 1.
DR   PIRSF; PIRSF015793; VAC_EEV; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   3: Inferred from homology;
KW   Host endosome; Late protein; Membrane; Reference proteome; Virion;
KW   Virulence.
FT   CHAIN           1..635
FT                   /note="Protein F12"
FT                   /id="PRO_0000099502"
SQ   SEQUENCE   635 AA;  73184 MW;  A72BFEF1A9CCF0B7 CRC64;
     MLNRVQILMK TANNYETIEI LRNYLRLYII LARNEEGHGI LIYDDNIDSI MSMMNITRLE
     VIGLTTHCTK LRSSPPIPMS RLFMDEIDHE SYYSPKTSDY PLIDIIRKRS HEQGDIALAL
     EQYGIENTDS ISEINEWLSS KGLACYRFVK FNDYRKQMYR KFSRCTIVDS MIIGHIGHHY
     IWIKNLETYT RPEIDVLPFD IKYISRDELW VRISSSLDQT HIKTIAVSVY GAITDNGPIP
     YMISTYPGNT FVNFNSVKNL ILNFLDWIKD IMTSTRTIIL VGYMSNLFDI PLLTVYWPNN
     CGWKIYNNTL ISSDGARVIW MDAYKFSCGL SLQDYCYHWG SKPESRPFDL IKKSDAKRNS
     KSLVKESMAS LKSLYEAFET QSGALEVLMS PCRMFSFSRI EDMFLTSVIN RVSENTGMGM
     YYPTNDIPSL FIESSICLDY IIVNNQESNK YRIKSVLDII SSKQYPAGRP NYVKNGTKGK
     LYIALCKVTV PTNDHIPVVY HDDDNTTTFI TVLTSVDIET AIRAGYSIVE LGALQWDNNI
     PELKNGLLDS IKMIYDLNAV TTNNLLEQLI ENINFNNSSI ISLFYTFAIS YCRAFIYSIM
     ETIDPVYISQ FSYKELYVSS SYKDINESMS QMVKL