ID   F12_VACCP               Reviewed;         635 AA.
AC   P29889; Q85335;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   23-FEB-2022, entry version 76.
DE   RecName: Full=Protein F12;
DE   AltName: Full=Protein F6;
GN   ORFNames=F12L, F6;
OS   Vaccinia virus (strain L-IVP) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=31531;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Mikryukov N.N., Chizhikov V.E., Prikhod'Ko G.G., Urmmanov I.M.,
RA   Serpinskii O.I., Blinov V.M., Nikulin A.E., Vasilenko S.K.;
RT   "Structural-functional organization of segment of vaccinia virus genome.";
RL   Biotekhnologiya 4:442-449(1988).
CC   -!- FUNCTION: Late protein that plays a role in intracellular enveloped
CC       virus (IEV) transport to the cell surface on microtubules. When absent,
CC       viral morphogenesis halts after the formation of IEV particles, and
CC       these are not transported to the cell periphery. Important for plaque
CC       formation, extracellular envelope virus (EEV) production and virulence
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}. Host endosome
CC       {ECO:0000250}. Note=Associates with the membrane of IEV particles, but
CC       not intracellular mature virus (IMV), cell-associated enveloped virus
CC       (CEV) or EEV. Colocalizes with microtubules (By similarity).
CC       {ECO:0000250}.
CC   -!- PTM: Not glycosylated or contains very little carbohydrate.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae F12 protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA48286.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M57977; AAA48285.1; -; Genomic_DNA.
DR   EMBL; M57977; AAA48286.1; ALT_INIT; Genomic_DNA.
DR   IntAct; P29889; 1.
DR   GO; GO:0044174; C:host cell endosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016032; P:viral process; IEA:InterPro.
DR   InterPro; IPR005005; Poxvirus_F12L.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   Pfam; PF03337; Pox_F12L; 1.
DR   PIRSF; PIRSF015793; VAC_EEV; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   3: Inferred from homology;
KW   Host endosome; Late protein; Membrane; Virion; Virulence.
FT   CHAIN           1..635
FT                   /note="Protein F12"
FT                   /id="PRO_0000099503"
SQ   SEQUENCE   635 AA;  73163 MW;  453034AB20450368 CRC64;
     MLNRIQTLMK TANNYETIEV LRNYLRLYII LARNEEGHGI LIYDDNIDSI MSMMNITRLE
     VIGLTTHCTK LRSSPPIPMS RLFMDEIDHE SYYSPKTSDY PLIDIIRKRS HEQGDIALAL
     ERYGIENTDS ISEINEWLSS KGLACYRFVK FNDYRKQMYR KFPRCTIVDS MIIGHIGHHY
     IWIKNLETYT RPEIDVLPFD IKYISRDELW ARISSSLDQT HIKTIAVSVY GAITDNGPMP
     YMISTYPGNT FVNFNSVKNL ILNFLDWIKD IMTSTRTIIL VGYMSNLFDI PLLTVYWPNN
     CGWKIYNNTL ISSDGARVIW MDAYKFSCGL SLQDYCYHWG SKPESRPFDL IKKSDAKRNS
     KSLVKESMAS LKSLYEAFET QSGASEVLMS PCRTFSFSRT EDTFSTSVIN RVFENTGTGT
     YHPINDIPSL FIESSICLDH IIVNNQESNK YRIKSVLDII SSKQYPARRP NYVKNGTKGK
     LYIALCKVTV PTNDHIPVVY HDDDNTTTFI TVLTSVDIET AIRVGYSIVE LGALQWDNNI
     PELKNGLLDS IKMIYDLNAV TTNNLLEQLI ENINFNNSSI ISLFYTFAIS YCRAFIYSIM
     ETIDPVYISQ FSYKELYVSS SYKDINESMS QMVKL