F12_VACCW
ID F12_VACCW Reviewed; 635 AA.
AC Q80HX6;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 29-SEP-2021, entry version 67.
DE RecName: Full=Protein F12;
GN OrderedLocusNames=VACWR051; ORFNames=F12L;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, AND LACK OF GLYCOSYLATION.
RX PubMed=11090164; DOI=10.1128/jvi.74.24.11654-11662.2000;
RA Zhang W.H., Wilcock D., Smith G.L.;
RT "Vaccinia virus F12L protein is required for actin tail formation, normal
RT plaque size, and virulence.";
RL J. Virol. 74:11654-11662(2000).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11752717; DOI=10.1099/0022-1317-83-1-195;
RA van Eijl H., Hollinshead M., Rodger G., Zhang W.-H., Smith G.L.;
RT "The vaccinia virus F12L protein is associated with intracellular enveloped
RT virus particles and is required for their egress to the cell surface.";
RL J. Gen. Virol. 83:195-207(2002).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16227217; DOI=10.1099/vir.0.81260-0;
RA Herrero-Martinez E., Roberts K.L., Hollinshead M., Smith G.L.;
RT "Vaccinia virus intracellular enveloped virions move to the cell periphery
RT on microtubules in the absence of the A36R protein.";
RL J. Gen. Virol. 86:2961-2968(2005).
RN [5]
RP INTERACTION WITH PROTEIN E2, AND FUNCTION.
RX PubMed=19207726; DOI=10.1111/j.1462-5822.2009.01296.x;
RA Dodding M.P., Newsome T.P., Collinson L.M., Edwards C., Way M.;
RT "An E2-F12 complex is required for intracellular enveloped virus
RT morphogenesis during vaccinia infection.";
RL Cell. Microbiol. 11:808-824(2009).
RN [6]
RP INTERACTION WITH PROTEIN A36, AND FUNCTION.
RX PubMed=20195521; DOI=10.1371/journal.ppat.1000785;
RA Morgan G.W., Hollinshead M., Ferguson B.J., Murphy B.J., Carpentier D.C.,
RA Smith G.L.;
RT "Vaccinia protein F12 has structural similarity to kinesin light chain and
RT contains a motor binding motif required for virion export.";
RL PLoS Pathog. 6:E1000785-E1000785(2010).
RN [7]
RP FUNCTION.
RX PubMed=25760349; DOI=10.1371/journal.ppat.1004723;
RA Carpentier D.C., Gao W.N., Ewles H., Morgan G.W., Smith G.L.;
RT "Vaccinia virus protein complex F12/E2 interacts with kinesin light chain
RT isoform 2 to engage the kinesin-1 motor complex.";
RL PLoS Pathog. 11:E1004723-E1004723(2015).
CC -!- FUNCTION: Late protein that plays a role in intracellular enveloped
CC virus (IEV) transport to the cell surface on microtubules. Together
CC with protein E2, forms a complex that interacts with host KLC2 (kinesin
CC light chain isoform 2) to engage the kinesin-1 complex and thereby
CC promote IEV trafficking. {ECO:0000269|PubMed:11090164,
CC ECO:0000269|PubMed:11752717, ECO:0000269|PubMed:16227217,
CC ECO:0000269|PubMed:19207726, ECO:0000269|PubMed:20195521}.
CC -!- SUBUNIT: Interacts with protein A36. Interacts with protein E2.
CC {ECO:0000269|PubMed:19207726, ECO:0000269|PubMed:20195521}.
CC -!- SUBCELLULAR LOCATION: Virion membrane. Host endosome. Note=Associates
CC with the membrane of IEV particles, but not intracellular mature virus
CC (IMV), cell-associated enveloped virus (CEV) or EEV. Colocalizes with
CC microtubules.
CC -!- PTM: Not glycosylated or contains very little carbohydrate.
CC -!- SIMILARITY: Belongs to the poxviridae F12 protein family.
CC {ECO:0000305}.
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DR EMBL; AY243312; AAO89330.1; -; Genomic_DNA.
DR RefSeq; YP_232933.1; NC_006998.1.
DR ELM; Q80HX6; -.
DR DNASU; 3707508; -.
DR GeneID; 3707508; -.
DR KEGG; vg:3707508; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0044174; C:host cell endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0039701; P:microtubule-dependent intracellular transport of viral material towards cell periphery; IDA:UniProtKB.
DR InterPro; IPR005005; Poxvirus_F12L.
DR InterPro; IPR012337; RNaseH-like_sf.
DR Pfam; PF03337; Pox_F12L; 1.
DR PIRSF; PIRSF015793; VAC_EEV; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 1: Evidence at protein level;
KW Host endosome; Host-virus interaction; Late protein; Membrane;
KW Microtubular outwards viral transport; Reference proteome;
KW Viral release from host cell; Virion; Virulence.
FT CHAIN 1..635
FT /note="Protein F12"
FT /id="PRO_0000343640"
SQ SEQUENCE 635 AA; 73217 MW; 5AF1EC870338D66D CRC64;
MLNRVQILMK TANNYETIEI LRNYLRLYII LARNEEGRGI LIYDDNIDSI MSMMNITRLE
VIGLTTHCTK LRSSPPIPMS RLFMDEIDHE SYYSPKTSDY PLIDIIRKRS HEQGDIALAL
EQYGIENTDS ISEINEWLSS KGLACYRFVK FNDYRKQMYR KFSRCTIVDS MIIGHIGHHY
IWIKNLETYT RPEIDVLPFD IKYISRDELW ARISSSLDQT HIKTIAVSVY GAITDNGPMP
YMISTYPGNT FVNFNSVKNL ILNFLDWIKD IMTSTRTIIL VGYMSNLFDI PLLTVYWPNN
CGWKIYNNTL ISSDGARVIW MDAYKFSCGL SLQDYCYHWG SKPESRPFDL IKKSDAKRNS
KSLVKESMAS LKSLYEAFET QSGALEVLMS PCRMFSFSRI EDMFLTSVIN RVSENTGMGM
YYPTNDIPSL FIESSICLDY IIVNNQESNK YRIKSVLDII SSKQYPAGRP NYVKNGTKGK
LYIALCKVTV PTNDHIPVVY HDDDNTTTFI TVLTSVDIET AIRAGYSIVE LGALQWDDNI
PELKHGLLDS IKMIYDLNAV TTNNLLEQLI ENINFNNSSI ISLFYTFAIS YCRAFIYSIM
ETIDPVYISQ FSYKELYVSS SYKDINESMS QMVKL
