ID   F12_VAR67               Reviewed;         635 AA.
AC   P33871;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Protein F12;
GN   ORFNames=C16L, F12L;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=India-1967 / Isolate Ind3;
RX   PubMed=8109158; DOI=10.1016/0168-1702(93)90093-3;
RA   Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V.,
RA   Sandakhchiev L.S.;
RT   "Analysis of the nucleotide sequence of a 43 kbp segment of the genome of
RT   variola virus India-1967 strain.";
RL   Virus Res. 30:239-258(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=India-1967 / Isolate Ind3;
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Late protein that plays a role in intracellular enveloped
CC       virus (IEV) transport to the cell surface on microtubules. When absent,
CC       viral morphogenesis halts after the formation of IEV particles, and
CC       these are not transported to the cell periphery. Important for plaque
CC       formation, extracellular envelope virus (EEV) production and virulence
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}. Host endosome
CC       {ECO:0000250}. Note=Associates with the membrane of IEV particles, but
CC       not intracellular mature virus (IMV), cell-associated enveloped virus
CC       (CEV) or EEV. Colocalizes with microtubules (By similarity).
CC       {ECO:0000250}.
CC   -!- PTM: Not glycosylated or contains very little carbohydrate.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae F12 protein family.
CC       {ECO:0000305}.
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DR   EMBL; X69198; CAA48977.1; -; Genomic_DNA.
DR   PIR; G36840; G36840.
DR   RefSeq; NP_042080.1; NC_001611.1.
DR   GeneID; 1486572; -.
DR   KEGG; vg:1486572; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0044174; C:host cell endosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016032; P:viral process; IEA:InterPro.
DR   InterPro; IPR005005; Poxvirus_F12L.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   Pfam; PF03337; Pox_F12L; 1.
DR   PIRSF; PIRSF015793; VAC_EEV; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   3: Inferred from homology;
KW   Host endosome; Late protein; Membrane; Reference proteome; Virion;
KW   Virulence.
FT   CHAIN           1..635
FT                   /note="Protein F12"
FT                   /id="PRO_0000099504"
SQ   SEQUENCE   635 AA;  73565 MW;  E7A0FFD415A85844 CRC64;
     MLNRVQILMK TANNYETIEI LRNYLRLYII LARNEEGHGI LIYDDNIDSI MSMMNITRLE
     VIGLTTHCTK LRSSPPIPMS RLFMDEIDHE SYYSPKTSDY PLIDIIRKRS HEQGDIALAL
     KRYCIENTDS ISEINEWLSS KGLACYRFVK FNDYRKQMYR KFSKCTIVDS MIIGHIGHHY
     IWIKNLETYT RPEIDVLPFD IKYISRDELW AQISSSLDQT HIKTITVSVY GAITDNGPMP
     YMISTYPGNT FVNFNSVKDL ILNFLDWIKD IMTSTRTIIL VGYMSNLFDI PLLTVYWPNN
     CGWKIYNNIL ISSDGARVIW MDAYKFSCGL SLQDYCYHWG SKPESRPFDL IKKIDAKRNI
     KSWVKESMTS LKSLYEAFET QSGALEVLMS PCRMFSFSRI EDMFLTSVIN RVSKNTGMGM
     YYPTNDISSL FIESSICLDY IIVNNQKSNK YRIKSVLDII SSKQYPAGRP NYVKNGTKGK
     LYIALCKVTV PTNDHIPVVY HDDDNTTTFI TVLTSVDIET ATRAGYSIVE LGALQWDDNI
     PELKDCLLDS IKMIYDLNTV TTNNLLEQLI ENINFNNSSI ILLFYTFAIS YCRAFIYSIM
     ETIDPVYISQ FSYKELYIRS SYKDINEVMS QMVKL