ID   F13_VAR67               Reviewed;         372 AA.
AC   P33815;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Envelope protein F13;
DE   AltName: Full=37 kDa protein;
DE   AltName: Full=Palmitoylated EV membrane protein;
DE   AltName: Full=p37K;
GN   ORFNames=C17L, F13L;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=India-1967 / Isolate Ind3;
RX   PubMed=8109158; DOI=10.1016/0168-1702(93)90093-3;
RA   Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V.,
RA   Sandakhchiev L.S.;
RT   "Analysis of the nucleotide sequence of a 43 kbp segment of the genome of
RT   variola virus India-1967 strain.";
RL   Virus Res. 30:239-258(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=India-1967 / Isolate Ind3;
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Envelope protein associated with the inner side of the
CC       enveloped virion (EV) membrane. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}; Lipid-anchor
CC       {ECO:0000250}. Host endoplasmic reticulum membrane {ECO:0000250};
CC       Lipid-anchor {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC       Note=Component of the inner side of the enveloped virion (EV) membrane.
CC       F13 is associated post-translationally with membranes.
CC   -!- DOMAIN: Late-budding domains (L domains) are short sequence motifs
CC       essential for viral particle budding. They recruit proteins of the host
CC       ESCRT machinery (Endosomal Sorting Complex Required for Transport) or
CC       ESCRT-associated proteins. F13 contains one L domain: a YPPL motif,
CC       which might interact with PDCD6IP/AIP1 (By similarity). {ECO:0000250}.
CC   -!- PTM: Palmitoylated. {ECO:0000250}.
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DR   EMBL; X69198; CAA48978.1; -; Genomic_DNA.
DR   PIR; H36840; H36840.
DR   RefSeq; NP_042081.1; NC_001611.1.
DR   SMR; P33815; -.
DR   DrugBank; DB12020; Tecovirimat.
DR   DrugCentral; P33815; -.
DR   GeneID; 1486573; -.
DR   KEGG; vg:1486573; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0044167; C:host cell endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0036338; C:viral membrane; ISS:UniProtKB.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0039702; P:viral budding via host ESCRT complex; IEA:UniProtKB-KW.
DR   InterPro; IPR032803; PLDc_3.
DR   InterPro; IPR001736; PLipase_D/transphosphatidylase.
DR   Pfam; PF00614; PLDc; 1.
DR   Pfam; PF13918; PLDc_3; 1.
DR   SMART; SM00155; PLDc; 2.
DR   PROSITE; PS50035; PLD; 1.
PE   3: Inferred from homology;
KW   Host endoplasmic reticulum; Host membrane; Host-virus interaction;
KW   Late protein; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Viral budding; Viral budding via the host ESCRT complexes;
KW   Viral envelope protein; Viral release from host cell; Virion.
FT   CHAIN           1..372
FT                   /note="Envelope protein F13"
FT                   /id="PRO_0000099199"
FT   DOMAIN          307..334
FT                   /note="PLD phosphodiesterase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00153"
FT   MOTIF           153..156
FT                   /note="YPPL"
SQ   SEQUENCE   372 AA;  41903 MW;  C769B05DD48EC944 CRC64;
     MWPFTSAPAG AKCRLVETLP ENMDFRSDHL TTFECFNEII TLAKKYIYIA SFCCNPLSTT
     RGALIFDKLK EASEKGIKII VLLDERGKRN LGELQSHCPD INFITVNIDK KNNVGLLLGC
     FWVSDDERCY VGNASFTGGS IHTIKTLGVY SDYPPLATDL RRRFDTFKAF NSVKNSWLNL
     YSSACCLPVS TAYHIKNPIG GVFFTDSPEH LLGYSRDLDT DVVIDKLRSA KTSIDIEHLA
     IVPTTRVDGN SYYWPDIYNS IIEAAINRGV KIRLLVGNWD KNDVYSMATA ESLDALCVQN
     DLSVKVFTIQ NNTKLLIVDD EYVHITSANF DGTHYQNHGF VSFNSIDKQL VSEAKKIFER
     DWVSSHSKSL KI