ID   AHLLM_AZOC5             Reviewed;         263 AA.
AC   A8IQD2;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=N-acyl homoserine lactonase AttM {ECO:0000250|UniProtKB:Q8VPD5};
DE            Short=AHL-lactonase AttM {ECO:0000250|UniProtKB:Q8VPD5};
DE            EC=3.1.1.81;
GN   Name=attM {ECO:0000250|UniProtKB:Q8VPD5}; OrderedLocusNames=AZC_0767;
OS   Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG
OS   6465 / NBRC 14845 / NCIMB 13405 / ORS 571).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Xanthobacteraceae; Azorhizobium.
OX   NCBI_TaxID=438753;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 / NCIMB
RC   13405 / ORS 571;
RA   Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T.,
RA   Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B.,
RA   Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.;
RT   "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium
RT   caulinodans ORS571.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine
CC         + H(+); Xref=Rhea:RHEA:22576, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:55474, ChEBI:CHEBI:58921; EC=3.1.1.81;
CC         Evidence={ECO:0000250|UniProtKB:Q8VPD5};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000250|UniProtKB:Q7B8B9};
CC       Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250|UniProtKB:Q7B8B9};
CC   -!- SIMILARITY: Belongs to the metallo-beta-lactamase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; AP009384; BAF86765.1; -; Genomic_DNA.
DR   RefSeq; WP_012169298.1; NC_009937.1.
DR   AlphaFoldDB; A8IQD2; -.
DR   SMR; A8IQD2; -.
DR   EnsemblBacteria; BAF86765; BAF86765; AZC_0767.
DR   KEGG; azc:AZC_0767; -.
DR   eggNOG; COG0491; Bacteria.
DR   HOGENOM; CLU_030571_3_2_5; -.
DR   OMA; HEEEFKH; -.
DR   OrthoDB; 1815576at2; -.
DR   Proteomes; UP000000270; Chromosome.
DR   GO; GO:0102007; F:acyl-L-homoserine-lactone lactonohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.60.15.10; -; 1.
DR   InterPro; IPR001279; Metallo-B-lactamas.
DR   InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR   Pfam; PF00753; Lactamase_B; 1.
DR   SMART; SM00849; Lactamase_B; 1.
DR   SUPFAM; SSF56281; SSF56281; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Plasmid; Reference proteome; Zinc.
FT   CHAIN           1..263
FT                   /note="N-acyl homoserine lactonase AttM"
FT                   /id="PRO_0000403297"
FT   BINDING         103
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         105
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         107
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         108
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         180
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         202
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         202
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         247
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
SQ   SEQUENCE   263 AA;  29317 MW;  67994DAE371A1AC9 CRC64;
     MTDIRLYMLQ SGSLKCKVHN IKMNQGNGAD YEIPVPFFLI THPKGHTIID GGNAVEVATD
     PRGHWGGVCD VYWPVMREDE GCVAQVKALG IDPADVKYVV QSHLHLDHTG AIGRFPNATH
     IVQRREYEYA FTPDWFAGGG YIRKDFDRPG LRWQFLNADV DDYYDIYGDG TLTTVFSPGH
     APGHQSFLVR LPKSGPLLLT IDAAYTLDHW NEQALPGFLA STVDTVRSVQ KLRTLAERTG
     AQVVTGHDPD AWPSFKKAPG YYD