AHLLM_RHIL3
ID AHLLM_RHIL3 Reviewed; 263 AA.
AC Q1M813;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=N-acyl homoserine lactonase AttM {ECO:0000250|UniProtKB:Q8VPD5};
DE Short=AHL-lactonase AttM {ECO:0000250|UniProtKB:Q8VPD5};
DE EC=3.1.1.81;
GN Name=attM {ECO:0000250|UniProtKB:Q8VPD5}; OrderedLocusNames=pRL100136;
OS Rhizobium leguminosarum bv. viciae (strain 3841).
OG Plasmid pRL10 {ECO:0000312|EMBL:CAK10359.1}.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=216596;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3841;
RX PubMed=16640791; DOI=10.1186/gb-2006-7-4-r34;
RA Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F.,
RA Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H.,
RA East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I.,
RA Chillingworth T., Clarke K., Cronin A., Davis P., Fraser A., Hance Z.,
RA Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J.;
RT "The genome of Rhizobium leguminosarum has recognizable core and accessory
RT components.";
RL Genome Biol. 7:R34.1-R34.20(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine
CC + H(+); Xref=Rhea:RHEA:22576, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:55474, ChEBI:CHEBI:58921; EC=3.1.1.81;
CC Evidence={ECO:0000250|UniProtKB:Q8VPD5};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000250|UniProtKB:Q7B8B9};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250|UniProtKB:Q7B8B9};
CC -!- SIMILARITY: Belongs to the metallo-beta-lactamase superfamily.
CC {ECO:0000305}.
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DR EMBL; AM236084; CAK10359.1; -; Genomic_DNA.
DR RefSeq; WP_011654183.1; NC_008381.1.
DR AlphaFoldDB; Q1M813; -.
DR SMR; Q1M813; -.
DR EnsemblBacteria; CAK10359; CAK10359; pRL100136.
DR KEGG; rle:pRL100136; -.
DR HOGENOM; CLU_030571_3_2_5; -.
DR OMA; HEEEFKH; -.
DR Proteomes; UP000006575; Plasmid pRL10.
DR GO; GO:0102007; F:acyl-L-homoserine-lactone lactonohydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.60.15.10; -; 1.
DR InterPro; IPR001279; Metallo-B-lactamas.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR Pfam; PF00753; Lactamase_B; 1.
DR SMART; SM00849; Lactamase_B; 1.
DR SUPFAM; SSF56281; SSF56281; 1.
PE 3: Inferred from homology;
KW Hydrolase; Metal-binding; Plasmid; Zinc.
FT CHAIN 1..263
FT /note="N-acyl homoserine lactonase AttM"
FT /id="PRO_0000403304"
FT BINDING 103
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT BINDING 105
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT BINDING 107
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT BINDING 108
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT BINDING 180
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT BINDING 202
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT BINDING 202
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT BINDING 247
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q7B8B9"
SQ SEQUENCE 263 AA; 29363 MW; C6E4B402C5E6C726 CRC64;
MTDIRLYMLQ SGTLKCKVHN IKMNQGNGAD YEIPVPFYLI THPDGHTIID GGNAIEVATD
PRGHWGGICD VYWPVLDKDK GCVDQVKALG FDPAEVRYVV QSHLHLDHTG AIGRFPNATH
IVQRAEYEYA FTPDWFAGGG YIRKDFDRPG LKWQFLNGTQ DDFYDVYGDG TLTTIFSPGH
AMGHQSFLVR LPSSEPLLLT IDAAYTLDHW EEKALPGFLA STVDTVRSVQ KLRTIAERTG
ANVVTGHDPA AWSTFKKAPE YYS
