F168B_HUMAN
ID F168B_HUMAN Reviewed; 195 AA.
AC A1KXE4; Q2TAZ6; Q6NZ40;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Myelin-associated neurite-outgrowth inhibitor;
DE Short=Mani;
DE AltName: Full=p20;
GN Name=FAM168B; Synonyms=KIAA0280L, MANI;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), HOMODIMERIZATION, INTERACTION WITH
RP CDC27; PRDX6 AND RBM6, AND TISSUE SPECIFICITY.
RX PubMed=20716133; DOI=10.1111/j.1582-4934.2010.01134.x;
RA Mishra M., Akatsu H., Heese K.;
RT "The novel protein MANI modulates neurogenesis and neurite-cone growth.";
RL J. Cell. Mol. Med. 15:1713-1725(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [5]
RP INTERACTION WITH DAZAP2; FAM168A; TMTC1 AND YPEL2.
RX PubMed=22771904; DOI=10.1016/j.febslet.2012.06.043;
RA Mishra M., Lee S., Lin M.K., Yamashita T., Heese K.;
RT "Characterizing the neurite outgrowth inhibitory effect of Mani.";
RL FEBS Lett. 586:3018-3023(2012).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Inhibitor of neuronal axonal outgrowth. Acts as a negative
CC regulator of CDC42 and STAT3 and a positive regulator of STMN2.
CC Positive regulator of CDC27. {ECO:0000250|UniProtKB:D4AEP3}.
CC -!- SUBUNIT: May form homodimers. May interact with DAZAP2, FAM168A, PRDX6,
CC RBM6, TMTC1 and YPEL2. Interacts with CDC27.
CC {ECO:0000269|PubMed:20716133, ECO:0000269|PubMed:22771904}.
CC -!- INTERACTION:
CC A1KXE4-2; P21549: AGXT; NbExp=3; IntAct=EBI-12193763, EBI-727098;
CC A1KXE4-2; Q03989: ARID5A; NbExp=3; IntAct=EBI-12193763, EBI-948603;
CC A1KXE4-2; O95817: BAG3; NbExp=3; IntAct=EBI-12193763, EBI-747185;
CC A1KXE4-2; Q9UQM7: CAMK2A; NbExp=3; IntAct=EBI-12193763, EBI-1383687;
CC A1KXE4-2; O75909-2: CCNK; NbExp=3; IntAct=EBI-12193763, EBI-12010594;
CC A1KXE4-2; P33240: CSTF2; NbExp=3; IntAct=EBI-12193763, EBI-711360;
CC A1KXE4-2; Q9H0L4: CSTF2T; NbExp=3; IntAct=EBI-12193763, EBI-747012;
CC A1KXE4-2; Q9H6Z9: EGLN3; NbExp=3; IntAct=EBI-12193763, EBI-1175354;
CC A1KXE4-2; Q92567-2: FAM168A; NbExp=3; IntAct=EBI-12193763, EBI-11978259;
CC A1KXE4-2; Q8WU58: FAM222B; NbExp=3; IntAct=EBI-12193763, EBI-2807642;
CC A1KXE4-2; P22607: FGFR3; NbExp=3; IntAct=EBI-12193763, EBI-348399;
CC A1KXE4-2; O75593: FOXH1; NbExp=3; IntAct=EBI-12193763, EBI-1759806;
CC A1KXE4-2; Q12951-2: FOXI1; NbExp=3; IntAct=EBI-12193763, EBI-12018822;
CC A1KXE4-2; Q9BZE0: GLIS2; NbExp=3; IntAct=EBI-12193763, EBI-7251368;
CC A1KXE4-2; Q13227: GPS2; NbExp=3; IntAct=EBI-12193763, EBI-713355;
CC A1KXE4-2; P17482: HOXB9; NbExp=3; IntAct=EBI-12193763, EBI-745290;
CC A1KXE4-2; Q9ULV5-2: HSF4; NbExp=3; IntAct=EBI-12193763, EBI-12056251;
CC A1KXE4-2; O14901: KLF11; NbExp=3; IntAct=EBI-12193763, EBI-948266;
CC A1KXE4-2; Q6PEX3: KRTAP26-1; NbExp=3; IntAct=EBI-12193763, EBI-3957672;
CC A1KXE4-2; Q14847-2: LASP1; NbExp=3; IntAct=EBI-12193763, EBI-9088686;
CC A1KXE4-2; Q71SY5: MED25; NbExp=3; IntAct=EBI-12193763, EBI-394558;
CC A1KXE4-2; Q8IXL7-2: MSRB3; NbExp=3; IntAct=EBI-12193763, EBI-10699187;
CC A1KXE4-2; Q8TDC0: MYOZ3; NbExp=3; IntAct=EBI-12193763, EBI-5662487;
CC A1KXE4-2; Q14511-2: NEDD9; NbExp=3; IntAct=EBI-12193763, EBI-11746523;
CC A1KXE4-2; Q9BVL2: NUP58; NbExp=3; IntAct=EBI-12193763, EBI-2811583;
CC A1KXE4-2; P32242: OTX1; NbExp=3; IntAct=EBI-12193763, EBI-740446;
CC A1KXE4-2; Q9HBE1-4: PATZ1; NbExp=3; IntAct=EBI-12193763, EBI-11022007;
CC A1KXE4-2; P78337: PITX1; NbExp=3; IntAct=EBI-12193763, EBI-748265;
CC A1KXE4-2; Q7Z3K3: POGZ; NbExp=3; IntAct=EBI-12193763, EBI-1389308;
CC A1KXE4-2; Q96HA1-2: POM121; NbExp=3; IntAct=EBI-12193763, EBI-11956563;
CC A1KXE4-2; Q7Z5V6-2: PPP1R32; NbExp=3; IntAct=EBI-12193763, EBI-12000762;
CC A1KXE4-2; P0CG20: PRR35; NbExp=3; IntAct=EBI-12193763, EBI-11986293;
CC A1KXE4-2; Q9BTL3: RAMAC; NbExp=3; IntAct=EBI-12193763, EBI-744023;
CC A1KXE4-2; O43251-10: RBFOX2; NbExp=3; IntAct=EBI-12193763, EBI-11963050;
CC A1KXE4-2; Q9BQY4: RHOXF2; NbExp=3; IntAct=EBI-12193763, EBI-372094;
CC A1KXE4-2; Q01974: ROR2; NbExp=3; IntAct=EBI-12193763, EBI-6422642;
CC A1KXE4-2; Q7Z3H4: SAMD7; NbExp=3; IntAct=EBI-12193763, EBI-12148649;
CC A1KXE4-2; P14678-2: SNRPB; NbExp=3; IntAct=EBI-12193763, EBI-372475;
CC A1KXE4-2; P09234: SNRPC; NbExp=3; IntAct=EBI-12193763, EBI-766589;
CC A1KXE4-2; Q99932-2: SPAG8; NbExp=3; IntAct=EBI-12193763, EBI-11959123;
CC A1KXE4-2; Q9NQB0-10: TCF7L2; NbExp=3; IntAct=EBI-12193763, EBI-11746252;
CC A1KXE4-2; Q96A09: TENT5B; NbExp=3; IntAct=EBI-12193763, EBI-752030;
CC A1KXE4-2; Q01085-2: TIAL1; NbExp=3; IntAct=EBI-12193763, EBI-11064654;
CC A1KXE4-2; Q08117-2: TLE5; NbExp=3; IntAct=EBI-12193763, EBI-11741437;
CC A1KXE4-2; O43711: TLX3; NbExp=3; IntAct=EBI-12193763, EBI-3939165;
CC A1KXE4-2; Q5T6F2: UBAP2; NbExp=3; IntAct=EBI-12193763, EBI-2514383;
CC A1KXE4-2; O95231: VENTX; NbExp=3; IntAct=EBI-12193763, EBI-10191303;
CC A1KXE4-2; Q14119: VEZF1; NbExp=3; IntAct=EBI-12193763, EBI-11980193;
CC A1KXE4-2; A8MV65-2: VGLL3; NbExp=3; IntAct=EBI-12193763, EBI-11957216;
CC A1KXE4-2; A5D8V6: VPS37C; NbExp=3; IntAct=EBI-12193763, EBI-2559305;
CC A1KXE4-2; Q64LD2-2: WDR25; NbExp=3; IntAct=EBI-12193763, EBI-12032042;
CC A1KXE4-2; Q96K80: ZC3H10; NbExp=3; IntAct=EBI-12193763, EBI-742550;
CC A1KXE4-2; Q15915: ZIC1; NbExp=3; IntAct=EBI-12193763, EBI-11963196;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC {ECO:0000250|UniProtKB:D4AEP3}. Cell membrane
CC {ECO:0000250|UniProtKB:Q80XQ8}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q80XQ8}. Cell projection, axon
CC {ECO:0000250|UniProtKB:Q80XQ8}. Note=Expressed in neuronal cell bodies
CC and axonal fibers. {ECO:0000250|UniProtKB:Q80XQ8}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A1KXE4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A1KXE4-2; Sequence=VSP_032508;
CC -!- TISSUE SPECIFICITY: Expressed in the brain, within neuronal axonal
CC fibers and associated with myelin sheets (at protein level). Expression
CC tends to be lower in the brain of Alzheimer disease patients compared
CC to healthy individuals (at protein level).
CC {ECO:0000269|PubMed:20716133}.
CC -!- PTM: N-glycosylated. {ECO:0000250|UniProtKB:D4AEP3}.
CC -!- SIMILARITY: Belongs to the FAM168 family. {ECO:0000305}.
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DR EMBL; AY253283; AAP79599.1; -; mRNA.
DR EMBL; CH471250; EAW51267.1; -; Genomic_DNA.
DR EMBL; BC066347; AAH66347.1; -; mRNA.
DR EMBL; BC110642; AAI10643.1; -; mRNA.
DR CCDS; CCDS42755.1; -. [A1KXE4-1]
DR RefSeq; NP_001009993.2; NM_001009993.3. [A1KXE4-1]
DR RefSeq; NP_001308672.1; NM_001321743.1. [A1KXE4-1]
DR RefSeq; NP_001308673.1; NM_001321744.1. [A1KXE4-1]
DR RefSeq; NP_001308674.1; NM_001321745.1. [A1KXE4-1]
DR RefSeq; NP_001308675.1; NM_001321746.1. [A1KXE4-1]
DR RefSeq; NP_001308676.1; NM_001321747.1. [A1KXE4-1]
DR RefSeq; NP_001308677.1; NM_001321748.1. [A1KXE4-1]
DR RefSeq; XP_016858816.1; XM_017003327.1. [A1KXE4-1]
DR AlphaFoldDB; A1KXE4; -.
DR BioGRID; 126221; 55.
DR IntAct; A1KXE4; 58.
DR MINT; A1KXE4; -.
DR STRING; 9606.ENSP00000387051; -.
DR GlyGen; A1KXE4; 1 site.
DR iPTMnet; A1KXE4; -.
DR PhosphoSitePlus; A1KXE4; -.
DR BioMuta; FAM168B; -.
DR EPD; A1KXE4; -.
DR jPOST; A1KXE4; -.
DR MassIVE; A1KXE4; -.
DR MaxQB; A1KXE4; -.
DR PaxDb; A1KXE4; -.
DR PeptideAtlas; A1KXE4; -.
DR PRIDE; A1KXE4; -.
DR ProteomicsDB; 124; -. [A1KXE4-1]
DR ProteomicsDB; 125; -. [A1KXE4-2]
DR Antibodypedia; 50282; 57 antibodies from 15 providers.
DR DNASU; 130074; -.
DR Ensembl; ENST00000389915.4; ENSP00000374565.3; ENSG00000152102.18. [A1KXE4-1]
DR Ensembl; ENST00000409185.5; ENSP00000387051.1; ENSG00000152102.18. [A1KXE4-1]
DR GeneID; 130074; -.
DR KEGG; hsa:130074; -.
DR MANE-Select; ENST00000389915.4; ENSP00000374565.3; NM_001009993.4; NP_001009993.2.
DR UCSC; uc002tsd.4; human. [A1KXE4-1]
DR CTD; 130074; -.
DR DisGeNET; 130074; -.
DR GeneCards; FAM168B; -.
DR HGNC; HGNC:27016; FAM168B.
DR HPA; ENSG00000152102; Low tissue specificity.
DR neXtProt; NX_A1KXE4; -.
DR OpenTargets; ENSG00000152102; -.
DR PharmGKB; PA162387098; -.
DR VEuPathDB; HostDB:ENSG00000152102; -.
DR eggNOG; ENOG502QQDS; Eukaryota.
DR GeneTree; ENSGT00390000005140; -.
DR HOGENOM; CLU_065824_1_0_1; -.
DR InParanoid; A1KXE4; -.
DR OMA; AMYAPHI; -.
DR PhylomeDB; A1KXE4; -.
DR TreeFam; TF331128; -.
DR PathwayCommons; A1KXE4; -.
DR SignaLink; A1KXE4; -.
DR BioGRID-ORCS; 130074; 14 hits in 1087 CRISPR screens.
DR ChiTaRS; FAM168B; human.
DR GenomeRNAi; 130074; -.
DR Pharos; A1KXE4; Tdark.
DR PRO; PR:A1KXE4; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; A1KXE4; protein.
DR Bgee; ENSG00000152102; Expressed in lateral globus pallidus and 210 other tissues.
DR ExpressionAtlas; A1KXE4; baseline and differential.
DR Genevisible; A1KXE4; HS.
DR GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR029247; FAM168A/MANI.
DR PANTHER; PTHR31844; PTHR31844; 1.
DR Pfam; PF14944; TCRP1; 2.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cell membrane; Cell projection;
KW Cytoplasm; Glycoprotein; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..195
FT /note="Myelin-associated neurite-outgrowth inhibitor"
FT /id="PRO_0000325978"
FT TOPO_DOM 1..18
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 19..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..142
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..195
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:19413330"
FT MOD_RES 6
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT CARBOHYD 46
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 78..195
FT /note="NPYQTAVYPVRSAYPQQSPYAQQGTYYTQPLYAAPPHVIHHTTVVQPNGMPA
FT TVYPAPIPPPRGNGVTMGMVAGTTMAMSAGTLLTAHSPTPVAPHPVTVPTYRAPGTPTY
FT SYVPPQW -> QAVPMSPCADQWARQLGQISGQPFVLLAGLLCWPHGEALESLMDCN
FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032508"
FT CONFLICT 7
FT /note="P -> L (in Ref. 3; AAI10643)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 195 AA; 20324 MW; 27A0D0D74AB44A10 CRC64;
MNPVYSPGSS GVPYANAKGI GYPAGFPMGY AAAAPAYSPN MYPGANPTFQ TGYTPGTPYK
VSCSPTSGAV PPYSSSPNPY QTAVYPVRSA YPQQSPYAQQ GTYYTQPLYA APPHVIHHTT
VVQPNGMPAT VYPAPIPPPR GNGVTMGMVA GTTMAMSAGT LLTAHSPTPV APHPVTVPTY
RAPGTPTYSY VPPQW
