F168B_MOUSE
ID F168B_MOUSE Reviewed; 194 AA.
AC Q80XQ8; Q3UNC7; Q5DTR7;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Myelin-associated neurite-outgrowth inhibitor;
DE Short=Mani;
GN Name=Fam168b; Synonyms=Kiaa4042;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Fetal brain;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Diencephalon;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, INTERACTION WITH CDC27, SUBCELLULAR LOCATION, INDUCTION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND GLYCOSYLATION.
RX PubMed=20716133; DOI=10.1111/j.1582-4934.2010.01134.x;
RA Mishra M., Akatsu H., Heese K.;
RT "The novel protein MANI modulates neurogenesis and neurite-cone growth.";
RL J. Cell. Mol. Med. 15:1713-1725(2011).
CC -!- FUNCTION: Inhibitor of neuronal axonal outgrowth. Acts as a negative
CC regulator of CDC42 and STAT3 and a positive regulator of STMN2.
CC Positive regulator of CDC27. {ECO:0000269|PubMed:20716133}.
CC -!- SUBUNIT: May form homodimers (By similarity). May interact with DAZAP2,
CC FAM168A, PRDX6, RBM6, TMTC1 and YPEL2 (By similarity). Interacts with
CC CDC27 (PubMed:20716133). {ECO:0000250|UniProtKB:A1KXE4,
CC ECO:0000269|PubMed:20716133}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC {ECO:0000250|UniProtKB:D4AEP3}. Cell membrane
CC {ECO:0000269|PubMed:20716133}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:20716133}. Cell projection, axon
CC {ECO:0000269|PubMed:20716133}. Note=Expressed in neuronal cell bodies
CC and axonal fibers. {ECO:0000269|PubMed:20716133}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the brain, including
CC olfactory bulb, cortex and cerebellum (at protein level).
CC {ECO:0000269|PubMed:20716133}.
CC -!- DEVELOPMENTAL STAGE: Specifically expressed in differentiated neurons,
CC but absent from proliferating neural stem cells.
CC {ECO:0000269|PubMed:20716133}.
CC -!- INDUCTION: Up-regulated by different neurotrophins, including NGF and
CC BDNF, but not by growth factors, such as EGF.
CC {ECO:0000269|PubMed:20716133}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:20716133}.
CC -!- SIMILARITY: Belongs to the FAM168 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD90278.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK220453; BAD90278.1; ALT_INIT; mRNA.
DR EMBL; AK144298; BAE25820.1; -; mRNA.
DR EMBL; BC040511; AAH40511.1; -; mRNA.
DR EMBL; BC043098; AAH43098.1; -; mRNA.
DR EMBL; BC059247; AAH59247.1; -; mRNA.
DR CCDS; CCDS14873.1; -.
DR RefSeq; NP_001153707.1; NM_001160235.1.
DR RefSeq; NP_001153708.1; NM_001160236.1.
DR RefSeq; NP_778162.1; NM_174997.2.
DR AlphaFoldDB; Q80XQ8; -.
DR GlyGen; Q80XQ8; 1 site.
DR iPTMnet; Q80XQ8; -.
DR PhosphoSitePlus; Q80XQ8; -.
DR MaxQB; Q80XQ8; -.
DR PRIDE; Q80XQ8; -.
DR ProteomicsDB; 275823; -.
DR Antibodypedia; 50282; 57 antibodies from 15 providers.
DR DNASU; 214469; -.
DR Ensembl; ENSMUST00000047534; ENSMUSP00000042212; ENSMUSG00000037503.
DR GeneID; 214469; -.
DR KEGG; mmu:214469; -.
DR UCSC; uc007apj.2; mouse.
DR CTD; 130074; -.
DR MGI; MGI:2448487; Fam168b.
DR VEuPathDB; HostDB:ENSMUSG00000037503; -.
DR eggNOG; ENOG502QQDS; Eukaryota.
DR GeneTree; ENSGT00390000005140; -.
DR HOGENOM; CLU_065824_1_0_1; -.
DR InParanoid; Q80XQ8; -.
DR OMA; AMYAPHI; -.
DR OrthoDB; 1348118at2759; -.
DR PhylomeDB; Q80XQ8; -.
DR TreeFam; TF331128; -.
DR BioGRID-ORCS; 214469; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Fam168b; mouse.
DR PRO; PR:Q80XQ8; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q80XQ8; protein.
DR Bgee; ENSMUSG00000037503; Expressed in otolith organ and 226 other tissues.
DR ExpressionAtlas; Q80XQ8; baseline and differential.
DR Genevisible; Q80XQ8; MM.
DR GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR029247; FAM168A/MANI.
DR PANTHER; PTHR31844; PTHR31844; 1.
DR Pfam; PF14944; TCRP1; 2.
PE 1: Evidence at protein level;
KW Acetylation; Cell membrane; Cell projection; Cytoplasm; Glycoprotein;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..194
FT /note="Myelin-associated neurite-outgrowth inhibitor"
FT /id="PRO_0000325979"
FT TOPO_DOM 1..18
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 19..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 42..141
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..194
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:A1KXE4"
FT MOD_RES 6
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:A1KXE4"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 49
FT /note="Q -> L (in Ref. 2; BAE25820)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 194 AA; 20194 MW; B59FCF5407B00518 CRC64;
MNPVYSPGSS GVPYANAKGI GYPAGFPVGY AAAPAYSPNM YPGANPTFQT GYTPGTPYKV
SCSPTSGAVP PYSSSPNPYQ TAVYPVRSAY PQQSPYAQQG TYYTQPLYAA PPHVIHHTTV
VQPNGMPATV YPAPIPPPRG SGVTMGMVAG TTMAMSAGTL LTAHSPTPVA PHPVTVPTYR
APGTPTYSYV PPQW
