F169A_HUMAN
ID F169A_HUMAN Reviewed; 670 AA.
AC Q9Y6X4; A8K1T9; Q6MZT0; Q9H989;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Soluble lamin-associated protein of 75 kDa;
DE Short=SLAP75;
DE AltName: Full=Protein FAM169A;
GN Name=FAM169A; Synonyms=KIAA0888;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=10048485; DOI=10.1093/dnares/5.6.355;
RA Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N.,
RA Tanaka A., Kotani H., Nomura N., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XII. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 5:355-364(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Hippocampus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Endometrium;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-449, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=22412018; DOI=10.1083/jcb.201112098;
RA Roux K.J., Kim D.I., Raida M., Burke B.;
RT "A promiscuous biotin ligase fusion protein identifies proximal and
RT interacting proteins in mammalian cells.";
RL J. Cell Biol. 196:801-810(2012).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- INTERACTION:
CC Q9Y6X4; P20700: LMNB1; NbExp=3; IntAct=EBI-1220497, EBI-968218;
CC Q9Y6X4; Q96QG7: MTMR9; NbExp=3; IntAct=EBI-1220497, EBI-744593;
CC -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000269|PubMed:22412018}.
CC Nucleus inner membrane {ECO:0000269|PubMed:22412018}; Peripheral
CC membrane protein {ECO:0000269|PubMed:22412018}; Nucleoplasmic side
CC {ECO:0000269|PubMed:22412018}. Note=Enriched at the nuclear lamina.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9Y6X4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y6X4-2; Sequence=VSP_031670, VSP_031671;
CC -!- SIMILARITY: Belongs to the FAM169 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA74911.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB14344.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAE45948.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB020695; BAA74911.1; ALT_INIT; mRNA.
DR EMBL; AK022981; BAB14344.1; ALT_INIT; mRNA.
DR EMBL; AK290004; BAF82693.1; -; mRNA.
DR EMBL; BX640902; CAE45948.1; ALT_INIT; mRNA.
DR CCDS; CCDS43330.1; -. [Q9Y6X4-1]
DR RefSeq; NP_056381.1; NM_015566.2. [Q9Y6X4-1]
DR RefSeq; XP_005248537.1; XM_005248480.2.
DR RefSeq; XP_011541608.1; XM_011543306.2.
DR RefSeq; XP_011541609.1; XM_011543307.2.
DR AlphaFoldDB; Q9Y6X4; -.
DR BioGRID; 117512; 30.
DR IntAct; Q9Y6X4; 7.
DR MINT; Q9Y6X4; -.
DR STRING; 9606.ENSP00000373808; -.
DR iPTMnet; Q9Y6X4; -.
DR MetOSite; Q9Y6X4; -.
DR PhosphoSitePlus; Q9Y6X4; -.
DR BioMuta; FAM169A; -.
DR DMDM; 189031482; -.
DR EPD; Q9Y6X4; -.
DR jPOST; Q9Y6X4; -.
DR MassIVE; Q9Y6X4; -.
DR MaxQB; Q9Y6X4; -.
DR PaxDb; Q9Y6X4; -.
DR PeptideAtlas; Q9Y6X4; -.
DR PRIDE; Q9Y6X4; -.
DR ProteomicsDB; 86814; -. [Q9Y6X4-1]
DR ProteomicsDB; 86815; -. [Q9Y6X4-2]
DR Antibodypedia; 48666; 10 antibodies from 9 providers.
DR DNASU; 26049; -.
DR Ensembl; ENST00000389156.9; ENSP00000373808.4; ENSG00000198780.13. [Q9Y6X4-1]
DR Ensembl; ENST00000510609.5; ENSP00000423905.1; ENSG00000198780.13. [Q9Y6X4-2]
DR Ensembl; ENST00000514215.5; ENSP00000425319.1; ENSG00000198780.13. [Q9Y6X4-2]
DR Ensembl; ENST00000687041.1; ENSP00000508577.1; ENSG00000198780.13. [Q9Y6X4-1]
DR GeneID; 26049; -.
DR KEGG; hsa:26049; -.
DR MANE-Select; ENST00000687041.1; ENSP00000508577.1; NM_001376049.1; NP_001362978.1.
DR UCSC; uc003kdm.4; human. [Q9Y6X4-1]
DR CTD; 26049; -.
DR GeneCards; FAM169A; -.
DR HGNC; HGNC:29138; FAM169A.
DR HPA; ENSG00000198780; Tissue enhanced (brain, retina).
DR MIM; 615769; gene.
DR neXtProt; NX_Q9Y6X4; -.
DR OpenTargets; ENSG00000198780; -.
DR PharmGKB; PA162387117; -.
DR VEuPathDB; HostDB:ENSG00000198780; -.
DR eggNOG; ENOG502QPRQ; Eukaryota.
DR GeneTree; ENSGT00510000048902; -.
DR HOGENOM; CLU_1969795_0_0_1; -.
DR InParanoid; Q9Y6X4; -.
DR OMA; YGGDQTH; -.
DR OrthoDB; 1122654at2759; -.
DR PhylomeDB; Q9Y6X4; -.
DR TreeFam; TF332578; -.
DR PathwayCommons; Q9Y6X4; -.
DR Reactome; R-HSA-9035034; RHOF GTPase cycle.
DR SignaLink; Q9Y6X4; -.
DR BioGRID-ORCS; 26049; 11 hits in 1067 CRISPR screens.
DR ChiTaRS; FAM169A; human.
DR GenomeRNAi; 26049; -.
DR Pharos; Q9Y6X4; Tdark.
DR PRO; PR:Q9Y6X4; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q9Y6X4; protein.
DR Bgee; ENSG00000198780; Expressed in caput epididymis and 164 other tissues.
DR ExpressionAtlas; Q9Y6X4; baseline and differential.
DR Genevisible; Q9Y6X4; HS.
DR GO; GO:0005637; C:nuclear inner membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR029625; FAM169.
DR InterPro; IPR029623; FAM169A.
DR PANTHER; PTHR22442; PTHR22442; 1.
DR PANTHER; PTHR22442:SF3; PTHR22442:SF3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..670
FT /note="Soluble lamin-associated protein of 75 kDa"
FT /id="PRO_0000320587"
FT REGION 273..301
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 314..670
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 314..332
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 337..351
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..373
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 394..427
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 481..519
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 527..541
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 556..590
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 602..633
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 634..651
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 652..670
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 350
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5XG69"
FT MOD_RES 379
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5XG69"
FT MOD_RES 449
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332"
FT MOD_RES 515
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5XG69"
FT MOD_RES 615
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5XG69"
FT MOD_RES 618
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5XG69"
FT MOD_RES 635
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT VAR_SEQ 107..127
FT /note="VSTLGERVVLYVLNRIIYRKQ -> EAYVPLFLPKVTNCQFLIQCF (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_031670"
FT VAR_SEQ 128..670
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_031671"
FT CONFLICT 275
FT /note="R -> G (in Ref. 2; BAF82693)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 670 AA; 74955 MW; 735D8883DA82C3F2 CRC64;
MAFPVDMLEN CSHEELENSA EDYMSDLRCG DPENPECFSL LNITIPISLS NVGFVPLYGG
DQTQKILALF APEDSLTAVA LYLADQWWAI DDIVKTSVPS REGLKQVSTL GERVVLYVLN
RIIYRKQEME RNEIPFLCHS STDYAKILWK KGEAIGFYSV KPTGSICASF LTQSYQLPVL
DTMFLRKKYR GKDFGLHMLE DFVDSFTEDA LGLRYPLSSL MYTACKQYFE KYPGDHELLW
EVEGVGHWYQ RIPVTRALQR EALKILALSQ NEPKRPMSGE YGPASVPEYE ARTEDNQSSE
MQLTIDSLKD AFASTSEGHD KTSVSTHTRS GNLKRPKIGK RFQDSEFSSS QGEDEKTSQT
SLTASINKLE STARPSESSE EFLEEEPEQR GIEFEDESSD RDARPALETQ PQQEKQDGEK
ESELEPMNGE IMDDSLKTSL ITEEEDSTSE VLDEELKLQP FNSSEDSTNL VPLVVESSKP
PEVDAPDKTP RIPDSEMLMD EGTSDEKGHM EEKLSLLPRK KAHLGSSDNV ATMSNEERSD
GGFPNSVIAE FSEEPVSENL SPNTTSSLED QGEEGVSEPQ ETSTALPQSS LIEVELEDVP
FSQNAGQKNQ SEEQSEASSE QLDQFTQSAE KAVDSSSEEI EVEVPVVDRR NLRRKAKGHK
GPAKKKAKLT
