F169A_MOUSE
ID F169A_MOUSE Reviewed; 665 AA.
AC Q5XG69; E9Q0D7; Q69ZW7;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Soluble lamin-associated protein of 75 kDa;
DE Short=SLAP75;
DE AltName: Full=Protein FAM169A;
GN Name=Fam169a; Synonyms=Kiaa0888;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Fetal brain;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryonic germ cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Teratocarcinoma;
RX PubMed=17622165; DOI=10.1021/pr070122r;
RA Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.;
RT "A differential phosphoproteomic analysis of retinoic acid-treated P19
RT cells.";
RL J. Proteome Res. 6:3174-3186(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-348; SER-377; SER-512;
RP SER-610; SER-613 AND SER-630, AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000250}. Nucleus inner
CC membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250};
CC Nucleoplasmic side {ECO:0000250}. Note=Enriched at the nuclear lamina.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the FAM169 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH84589.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAD32329.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK173051; BAD32329.1; ALT_INIT; mRNA.
DR EMBL; AC154620; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC084589; AAH84589.1; ALT_INIT; mRNA.
DR CCDS; CCDS49337.1; -.
DR RefSeq; NP_001093928.1; NM_001100458.1.
DR RefSeq; NP_001139517.1; NM_001146045.1.
DR RefSeq; XP_006517749.1; XM_006517686.3.
DR RefSeq; XP_011242971.1; XM_011244669.2.
DR AlphaFoldDB; Q5XG69; -.
DR BioGRID; 236114; 7.
DR STRING; 10090.ENSMUSP00000043738; -.
DR iPTMnet; Q5XG69; -.
DR PhosphoSitePlus; Q5XG69; -.
DR MaxQB; Q5XG69; -.
DR PaxDb; Q5XG69; -.
DR PeptideAtlas; Q5XG69; -.
DR PRIDE; Q5XG69; -.
DR ProteomicsDB; 275972; -.
DR Antibodypedia; 48666; 10 antibodies from 9 providers.
DR Ensembl; ENSMUST00000042517; ENSMUSP00000043738; ENSMUSG00000041817.
DR Ensembl; ENSMUST00000169863; ENSMUSP00000126209; ENSMUSG00000041817.
DR GeneID; 320557; -.
DR KEGG; mmu:320557; -.
DR UCSC; uc007rnr.2; mouse.
DR CTD; 26049; -.
DR MGI; MGI:2444268; Fam169a.
DR VEuPathDB; HostDB:ENSMUSG00000041817; -.
DR eggNOG; ENOG502QPRQ; Eukaryota.
DR GeneTree; ENSGT00510000048902; -.
DR HOGENOM; CLU_026572_1_0_1; -.
DR InParanoid; Q5XG69; -.
DR OMA; YGGDQTH; -.
DR OrthoDB; 1122654at2759; -.
DR PhylomeDB; Q5XG69; -.
DR TreeFam; TF332578; -.
DR Reactome; R-MMU-9035034; RHOF GTPase cycle.
DR BioGRID-ORCS; 320557; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Fam169a; mouse.
DR PRO; PR:Q5XG69; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q5XG69; protein.
DR Bgee; ENSMUSG00000041817; Expressed in retinal neural layer and 153 other tissues.
DR Genevisible; Q5XG69; MM.
DR GO; GO:0005637; C:nuclear inner membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR029625; FAM169.
DR InterPro; IPR029623; FAM169A.
DR PANTHER; PTHR22442; PTHR22442; 1.
DR PANTHER; PTHR22442:SF3; PTHR22442:SF3; 1.
PE 1: Evidence at protein level;
KW Membrane; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..665
FT /note="Soluble lamin-associated protein of 75 kDa"
FT /id="PRO_0000320588"
FT REGION 309..665
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 341..371
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 480..495
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 497..513
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 514..539
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 553..568
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 629..646
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 647..665
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 348
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 377
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 447
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6X4"
FT MOD_RES 512
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 610
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 613
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 630
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 112
FT /note="E -> G (in Ref. 3; AAH84589)"
FT /evidence="ECO:0000305"
FT CONFLICT 339
FT /note="H -> R (in Ref. 1; BAD32329)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 665 AA; 73240 MW; 37A5F99B1CE456BF CRC64;
MAFPVDLLDN CTHEELENSS EDYLSSLRCG DPEHPECFSS LNITIPVSLS NVGFVPLYGG
NQTQKILALF APEDSLTAVA LYLVGQWWAI DDIVKTSEPS REGLKQVSTL GERVVLYVLN
RIIYRKQEME RNEIPFLCHS STDYAKILWK KGEAVGFYSV KPTGSLCASF LTQNYQLPVL
DTMFIRKKYR GKDLGLHMLE DFVDSFTEDA LGLRYPLSSL MYTASKQYFE KYPGDHELLW
EVEGVGHWHQ RVPVTRALQR EAIKATDVSQ YEATRPVSGE YGLAAVPEHE PGLDDTQSSE
LQIHSLKDAF ASTSEGPEKT PVSTRTRSSH LKRPKIGKHF QDSEFSSSQG EDENVAKTSP
TASVNKIEYA ARTSESSEEF LEEEPEQGVI DFEDESGDKD AQPALETQPR LQKQDGDKDS
ALEPVNGEVM DAALKPSLTT EDEDSTSEGL EEDLKVPPFN SSGEPGNPVP LVAESSKVPE
ATLAKTSPDT DSEMLIDQSP SDDKGHTEEN LSPVSKKKTL LGSSDNVATV SNIEKSDGNF
PNSVVPEFPE EPVSQNLSPN TTSSVEDQGE EGAPEAQEPS ATQSSLIEVE LEDAPFPQNA
GQKSQSEEQS EASSEHLEQF TQSAEKAVDS SSEEIEVEVP VVDRRNLRRK AKGHKGPGKK
KAKLT
