AHP1_ARATH
ID AHP1_ARATH Reviewed; 154 AA.
AC Q9ZNV9;
DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Histidine-containing phosphotransfer protein 1;
GN Name=AHP1; Synonyms=ATHP3; OrderedLocusNames=At3g21510; ORFNames=MIL23.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=9804162; DOI=10.1016/s0014-5793(98)01188-0;
RA Miyata S., Urao T., Yamaguchi-Shinozaki K., Shinozaki K.;
RT "Characterization of genes for two-component phosphorelay mediators with a
RT single HPt domain in Arabidopsis thaliana.";
RL FEBS Lett. 437:11-14(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=10050311; DOI=10.1093/oxfordjournals.pcp.a029329;
RA Suzuki T., Imamura A., Ueguchi C., Mizuno T.;
RT "Histidine-containing phosphotransfer (HPt) signal transducers implicated
RT in His-to-Asp phosphorelay in Arabidopsis.";
RL Plant Cell Physiol. 39:1258-1268(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP INTERACTION WITH ARR4; ARR9 AND ETR1.
RX PubMed=10930573; DOI=10.1016/s0014-5793(00)01860-3;
RA Urao T., Miyata S., Yamaguchi-Shinozaki K., Shinozaki K.;
RT "Possible His to Asp phosphorelay signaling in an Arabidopsis two-component
RT system.";
RL FEBS Lett. 478:227-232(2000).
RN [7]
RP INTERACTION.
RX PubMed=11158442; DOI=10.1093/pcp/pce011;
RA Suzuki T., Sakurai K., Ueguchi C., Mizuno T.;
RT "Two types of putative nuclear factors that physically interact with
RT histidine-containing phosphotransfer (Hpt) domains, signaling mediators in
RT His-to-Asp phosphorelay, in Arabidopsis thaliana.";
RL Plant Cell Physiol. 42:37-45(2001).
RN [8]
RP INTERACTION WITH AHK4.
RX PubMed=11230563; DOI=10.1093/pcp/pce037;
RA Suzuki T., Miwa K., Ishikawa K., Yamada H., Aiba H., Mizuno T.;
RT "The Arabidopsis sensor His-kinase, AHk4, can respond to cytokinins.";
RL Plant Cell Physiol. 42:107-113(2001).
RN [9]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12068096; DOI=10.1104/pp.005504;
RA Hwang I., Chen H.-C., Sheen J.;
RT "Two-component signal transduction pathways in Arabidopsis.";
RL Plant Physiol. 129:500-515(2002).
RN [10]
RP FUNCTION, TISSUE SPECIFICITY, AND INTERACTION.
RX PubMed=14981318; DOI=10.1271/bbb.68.462;
RA Tanaka Y., Suzuki T., Yamashino T., Mizuno T.;
RT "Comparative studies of the AHP histidine-containing phosphotransmitters
RT implicated in His-to-Asp phosphorelay in Arabidopsis thaliana.";
RL Biosci. Biotechnol. Biochem. 68:462-465(2004).
RN [11]
RP INTERACTION WITH AHK2; AHK3 AND AHK4.
RX PubMed=16965536; DOI=10.1111/j.1742-4658.2006.05467.x;
RA Dortay H., Mehnert N., Buerkle L., Schmuelling T., Heyl A.;
RT "Analysis of protein interactions within the cytokinin-signaling pathway of
RT Arabidopsis thaliana.";
RL FEBS J. 273:4631-4644(2006).
RN [12]
RP SUBCELLULAR LOCATION.
RX PubMed=18642946; DOI=10.1021/pr0703831;
RA Dortay H., Gruhn N., Pfeifer A., Schwerdtner M., Schmuelling T., Heyl A.;
RT "Toward an interaction map of the two-component signaling pathway of
RT Arabidopsis thaliana.";
RL J. Proteome Res. 7:3649-3660(2008).
RN [13]
RP INTERACTION WITH FBR12 AND AHK4.
RX PubMed=24163315; DOI=10.1105/tpc.113.116236;
RA Ren B., Chen Q., Hong S., Zhao W., Feng J., Feng H., Zuo J.;
RT "The Arabidopsis eukaryotic translation initiation factor eIF5A-2 regulates
RT root protoxylem development by modulating cytokinin signaling.";
RL Plant Cell 25:3841-3857(2013).
RN [14]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH AHK5, AND
RP INTERACTION WITH AHK5.
RX PubMed=23132142; DOI=10.1093/mp/sss126;
RA Bauer J., Reiss K., Veerabagu M., Heunemann M., Harter K., Stehle T.;
RT "Structure-function analysis of Arabidopsis thaliana histidine kinase AHK5
RT bound to its cognate phosphotransfer protein AHP1.";
RL Mol. Plant 6:959-970(2013).
CC -!- FUNCTION: Functions as two-component phosphorelay mediators between
CC cytokinin sensor histidine kinases and response regulators (B-type
CC ARRs). Plays an important role in propagating cytokinin signal
CC transduction through the multistep His-to-Asp phosphorelay.
CC {ECO:0000269|PubMed:10050311, ECO:0000269|PubMed:12068096,
CC ECO:0000269|PubMed:14981318, ECO:0000269|PubMed:9804162}.
CC -!- SUBUNIT: Interacts with the B-type response regulators ARR1, ARR2, ARR4
CC and ARR9. Binds to ETR1, AHK2, AHK3, AHK4, AHK5 and FBR12.
CC {ECO:0000269|PubMed:10930573, ECO:0000269|PubMed:11158442,
CC ECO:0000269|PubMed:11230563, ECO:0000269|PubMed:14981318,
CC ECO:0000269|PubMed:16965536, ECO:0000269|PubMed:23132142,
CC ECO:0000269|PubMed:24163315}.
CC -!- INTERACTION:
CC Q9ZNV9; Q9C5U2: AHK2; NbExp=2; IntAct=EBI-1100673, EBI-1100634;
CC Q9ZNV9; Q9C5U1: AHK3; NbExp=2; IntAct=EBI-1100673, EBI-1100653;
CC Q9ZNV9; Q940D0: ARR1; NbExp=3; IntAct=EBI-1100673, EBI-1100998;
CC Q9ZNV9; Q8L9Y3: ARR14; NbExp=2; IntAct=EBI-1100673, EBI-1100737;
CC Q9ZNV9; Q9ZWJ9: ARR2; NbExp=4; IntAct=EBI-1100673, EBI-1101028;
CC Q9ZNV9; Q9SB04: ARR5; NbExp=2; IntAct=EBI-1100673, EBI-1100883;
CC Q9ZNV9; O80366: ARR9; NbExp=3; IntAct=EBI-1100673, EBI-1100950;
CC Q9ZNV9; P49333: ETR1; NbExp=3; IntAct=EBI-1100673, EBI-1606682;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:18642946}.
CC Nucleus {ECO:0000269|PubMed:18642946}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in roots.
CC {ECO:0000269|PubMed:14981318, ECO:0000269|PubMed:9804162}.
CC -!- INDUCTION: By salt, cold and drought stress.
CC {ECO:0000269|PubMed:9804162}.
CC -!- DOMAIN: Histidine-containing phosphotransfer domain (HPt) contains an
CC active histidine that mediates the phosphotransfer.
CC -!- PTM: Two-component system major event consists of a His-to-Asp
CC phosphorelay between a sensor histidine kinase (HK) and a response
CC regulator (RR). In plants, the His-to-Asp phosphorelay involves an
CC additional intermediate named Histidine-containing phosphotransfer
CC protein (HPt). This multistep phosphorelay consists of a His-Asp-His-
CC Asp sequential transfer of a phosphate group between first an His and
CC an Asp of the HK protein, followed by the transfer to a conserved His
CC of the HPt protein and finally the transfer to an Asp in the receiver
CC domain of the RR protein.
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DR EMBL; AB012570; BAA37112.1; -; mRNA.
DR EMBL; AB015141; BAA36335.1; -; mRNA.
DR EMBL; AB019232; BAB02346.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76518.1; -; Genomic_DNA.
DR EMBL; AF370265; AAK44080.1; -; mRNA.
DR EMBL; AY063082; AAL34256.1; -; mRNA.
DR RefSeq; NP_188788.1; NM_113046.4.
DR PDB; 4EUK; X-ray; 1.95 A; B=1-154.
DR PDBsum; 4EUK; -.
DR AlphaFoldDB; Q9ZNV9; -.
DR SMR; Q9ZNV9; -.
DR BioGRID; 7037; 33.
DR IntAct; Q9ZNV9; 25.
DR STRING; 3702.AT3G21510.1; -.
DR PaxDb; Q9ZNV9; -.
DR PRIDE; Q9ZNV9; -.
DR ProteomicsDB; 244803; -.
DR DNASU; 821705; -.
DR EnsemblPlants; AT3G21510.1; AT3G21510.1; AT3G21510.
DR GeneID; 821705; -.
DR Gramene; AT3G21510.1; AT3G21510.1; AT3G21510.
DR KEGG; ath:AT3G21510; -.
DR Araport; AT3G21510; -.
DR TAIR; locus:2089900; AT3G21510.
DR eggNOG; KOG4747; Eukaryota.
DR HOGENOM; CLU_111777_1_0_1; -.
DR InParanoid; Q9ZNV9; -.
DR OMA; VEACHRC; -.
DR OrthoDB; 1488145at2759; -.
DR PhylomeDB; Q9ZNV9; -.
DR PRO; PR:Q9ZNV9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9ZNV9; baseline and differential.
DR Genevisible; Q9ZNV9; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0009927; F:histidine phosphotransfer kinase activity; IDA:TAIR.
DR GO; GO:0043424; F:protein histidine kinase binding; IPI:UniProtKB.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IMP:TAIR.
DR GO; GO:0009553; P:embryo sac development; IGI:TAIR.
DR GO; GO:0009825; P:multidimensional cell growth; IGI:TAIR.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IMP:TAIR.
DR GO; GO:0016310; P:phosphorylation; IBA:GO_Central.
DR CDD; cd00088; HPT; 1.
DR Gene3D; 1.20.120.160; -; 1.
DR InterPro; IPR045871; AHP1-5/YPD1.
DR InterPro; IPR036641; HPT_dom_sf.
DR InterPro; IPR008207; Sig_transdc_His_kin_Hpt_dom.
DR PANTHER; PTHR28242; PTHR28242; 1.
DR Pfam; PF01627; Hpt; 1.
DR SUPFAM; SSF47226; SSF47226; 1.
DR PROSITE; PS50894; HPT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cytokinin signaling pathway; Cytoplasm; Nucleus;
KW Phosphoprotein; Reference proteome; Stress response;
KW Two-component regulatory system.
FT CHAIN 1..154
FT /note="Histidine-containing phosphotransfer protein 1"
FT /id="PRO_0000074927"
FT DOMAIN 38..143
FT /note="HPt"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00110"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9ZNV8"
FT MOD_RES 79
FT /note="Phosphohistidine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00110"
FT HELIX 3..19
FT /evidence="ECO:0007829|PDB:4EUK"
FT HELIX 25..33
FT /evidence="ECO:0007829|PDB:4EUK"
FT HELIX 41..65
FT /evidence="ECO:0007829|PDB:4EUK"
FT STRAND 66..68
FT /evidence="ECO:0007829|PDB:4EUK"
FT HELIX 71..88
FT /evidence="ECO:0007829|PDB:4EUK"
FT HELIX 91..105
FT /evidence="ECO:0007829|PDB:4EUK"
FT HELIX 109..142
FT /evidence="ECO:0007829|PDB:4EUK"
SQ SEQUENCE 154 AA; 17615 MW; BA0C026EA19AF83D CRC64;
MDLVQKQKSL QDYTKSLFLE GILDSQFLQL QQLQDESNPD FVSQVVTLFF QDSDRILNDL
SLSLDQQVVD FKKVDPHVHQ LKGSSSSIGA QRVKNACVVF RSFCEQQNVE ACHRCLQQVK
QEYYLVKNRL ETLFKLEQQI VASGGMIPAV ELGF
