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AHP2_ARATH
ID   AHP2_ARATH              Reviewed;         156 AA.
AC   Q9ZNV8; Q3EAX3;
DT   05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 156.
DE   RecName: Full=Histidine-containing phosphotransfer protein 2;
GN   Name=AHP2; Synonyms=ATHP1; OrderedLocusNames=At3g29350; ORFNames=MUO10.6;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Columbia;
RX   PubMed=9804162; DOI=10.1016/s0014-5793(98)01188-0;
RA   Miyata S., Urao T., Yamaguchi-Shinozaki K., Shinozaki K.;
RT   "Characterization of genes for two-component phosphorelay mediators with a
RT   single HPt domain in Arabidopsis thaliana.";
RL   FEBS Lett. 437:11-14(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC   STRAIN=cv. Columbia;
RX   PubMed=10050311; DOI=10.1093/oxfordjournals.pcp.a029329;
RA   Suzuki T., Imamura A., Ueguchi C., Mizuno T.;
RT   "Histidine-containing phosphotransfer (HPt) signal transducers implicated
RT   in His-to-Asp phosphorelay in Arabidopsis.";
RL   Plant Cell Physiol. 39:1258-1268(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT   features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT   clones.";
RL   DNA Res. 7:217-221(2000).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [6]
RP   INTERACTION WITH AHK1; ETR1 AND CKI1.
RX   PubMed=10930573; DOI=10.1016/s0014-5793(00)01860-3;
RA   Urao T., Miyata S., Yamaguchi-Shinozaki K., Shinozaki K.;
RT   "Possible His to Asp phosphorelay signaling in an Arabidopsis two-component
RT   system.";
RL   FEBS Lett. 478:227-232(2000).
RN   [7]
RP   INTERACTION.
RX   PubMed=11158442; DOI=10.1093/pcp/pce011;
RA   Suzuki T., Sakurai K., Ueguchi C., Mizuno T.;
RT   "Two types of putative nuclear factors that physically interact with
RT   histidine-containing phosphotransfer (Hpt) domains, signaling mediators in
RT   His-to-Asp phosphorelay, in Arabidopsis thaliana.";
RL   Plant Cell Physiol. 42:37-45(2001).
RN   [8]
RP   INTERACTION WITH AHK4.
RX   PubMed=11230563; DOI=10.1093/pcp/pce037;
RA   Suzuki T., Miwa K., Ishikawa K., Yamada H., Aiba H., Mizuno T.;
RT   "The Arabidopsis sensor His-kinase, AHk4, can respond to cytokinins.";
RL   Plant Cell Physiol. 42:107-113(2001).
RN   [9]
RP   GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX   PubMed=12068096; DOI=10.1104/pp.005504;
RA   Hwang I., Chen H.-C., Sheen J.;
RT   "Two-component signal transduction pathways in Arabidopsis.";
RL   Plant Physiol. 129:500-515(2002).
RN   [10]
RP   FUNCTION, TISSUE SPECIFICITY, AND INTERACTION.
RX   PubMed=14981318; DOI=10.1271/bbb.68.462;
RA   Tanaka Y., Suzuki T., Yamashino T., Mizuno T.;
RT   "Comparative studies of the AHP histidine-containing phosphotransmitters
RT   implicated in His-to-Asp phosphorelay in Arabidopsis thaliana.";
RL   Biosci. Biotechnol. Biochem. 68:462-465(2004).
RN   [11]
RP   INTERACTION WITH AHK2; AHK3 AND AHK4.
RX   PubMed=16965536; DOI=10.1111/j.1742-4658.2006.05467.x;
RA   Dortay H., Mehnert N., Buerkle L., Schmuelling T., Heyl A.;
RT   "Analysis of protein interactions within the cytokinin-signaling pathway of
RT   Arabidopsis thaliana.";
RL   FEBS J. 273:4631-4644(2006).
RN   [12]
RP   INTERACTION WITH AHK2 AND AHK4, AND SUBCELLULAR LOCATION.
RX   PubMed=18642946; DOI=10.1021/pr0703831;
RA   Dortay H., Gruhn N., Pfeifer A., Schwerdtner M., Schmuelling T., Heyl A.;
RT   "Toward an interaction map of the two-component signaling pathway of
RT   Arabidopsis thaliana.";
RL   J. Proteome Res. 7:3649-3660(2008).
RN   [13]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA   Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA   Giglione C.;
RT   "Comparative large-scale characterisation of plant vs. mammal proteins
RT   reveals similar and idiosyncratic N-alpha acetylation features.";
RL   Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN   [14]
RP   INTERACTION WITH AHK5.
RX   PubMed=23132142; DOI=10.1093/mp/sss126;
RA   Bauer J., Reiss K., Veerabagu M., Heunemann M., Harter K., Stehle T.;
RT   "Structure-function analysis of Arabidopsis thaliana histidine kinase AHK5
RT   bound to its cognate phosphotransfer protein AHP1.";
RL   Mol. Plant 6:959-970(2013).
RN   [15]
RP   X-RAY CRYSTALLOGRAPHY (2.53 ANGSTROMS).
RA   Degtjarik O., Dopitova R., Puehringer S., Reha D., Otrusinova O.,
RA   Pekarova B., Kuty M., Zidek L., Janda L., Weiss M.S., Kuta-Smatanova I.,
RA   Hejatko H.;
RT   "Crystal structure of AHP2 from Arabidopsis thaliana.";
RL   Submitted (APR-2014) to the PDB data bank.
CC   -!- FUNCTION: Functions as two-component phosphorelay mediators between
CC       cytokinin sensor histidine kinases and response regulators (B-type
CC       ARRs). Plays an important role in propagating cytokinin signal
CC       transduction through the multistep His-to-Asp phosphorelay.
CC       {ECO:0000269|PubMed:10050311, ECO:0000269|PubMed:12068096,
CC       ECO:0000269|PubMed:14981318, ECO:0000269|PubMed:9804162}.
CC   -!- SUBUNIT: Interacts with the B-type response regulators ARR1, ARR2 and
CC       ARR10. Binds to AHK1, AHK2, AHK3, AHK4, AHK5, ETR1 and CKI1.
CC       {ECO:0000269|PubMed:10930573, ECO:0000269|PubMed:11158442,
CC       ECO:0000269|PubMed:11230563, ECO:0000269|PubMed:14981318,
CC       ECO:0000269|PubMed:16965536, ECO:0000269|PubMed:18642946,
CC       ECO:0000269|PubMed:23132142}.
CC   -!- INTERACTION:
CC       Q9ZNV8; Q9C5U2: AHK2; NbExp=3; IntAct=EBI-1100687, EBI-1100634;
CC       Q9ZNV8; Q9C5U1: AHK3; NbExp=2; IntAct=EBI-1100687, EBI-1100653;
CC       Q9ZNV8; O49397: ARR10; NbExp=3; IntAct=EBI-1100687, EBI-1101329;
CC       Q9ZNV8; Q8L9Y3: ARR14; NbExp=3; IntAct=EBI-1100687, EBI-1100737;
CC       Q9ZNV8; Q9SHC2: ARR16; NbExp=2; IntAct=EBI-1100687, EBI-1100982;
CC       Q9ZNV8; Q9ZWJ9: ARR2; NbExp=7; IntAct=EBI-1100687, EBI-1101028;
CC       Q9ZNV8; Q9ZWS7: ARR7; NbExp=6; IntAct=EBI-1100687, EBI-1100917;
CC       Q9ZNV8; O80366: ARR9; NbExp=4; IntAct=EBI-1100687, EBI-1100950;
CC       Q9ZNV8; Q39009: DMC1; NbExp=2; IntAct=EBI-1100687, EBI-307715;
CC       Q9ZNV8; Q9ZR37: DSPTP1; NbExp=3; IntAct=EBI-1100687, EBI-25512239;
CC       Q9ZNV8; Q8GYD2: MND1; NbExp=2; IntAct=EBI-1100687, EBI-1554720;
CC       Q9ZNV8; O23160: MYB73; NbExp=3; IntAct=EBI-1100687, EBI-25506855;
CC       Q9ZNV8; Q39204: MYC2; NbExp=4; IntAct=EBI-1100687, EBI-1792336;
CC       Q9ZNV8; Q9LQF0: TCP23; NbExp=3; IntAct=EBI-1100687, EBI-15192297;
CC       Q9ZNV8; Q9LMA8: TIFY10A; NbExp=3; IntAct=EBI-1100687, EBI-1388539;
CC       Q9ZNV8; Q9LDU5: TIFY11A; NbExp=3; IntAct=EBI-1100687, EBI-2312095;
CC       Q9ZNV8; Q8GY55: TIFY4B; NbExp=3; IntAct=EBI-1100687, EBI-15206004;
CC       Q9ZNV8; Q84MB2: TIFY8; NbExp=3; IntAct=EBI-1100687, EBI-4426557;
CC       Q9ZNV8; Q9SK33: WRKY60; NbExp=3; IntAct=EBI-1100687, EBI-2112777;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:18642946}.
CC       Nucleus {ECO:0000269|PubMed:18642946}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9ZNV8-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9ZNV8-2; Sequence=VSP_018143;
CC   -!- TISSUE SPECIFICITY: Strongly expressed in flowers and roots. Detected
CC       also in leaves, siliques and stems. {ECO:0000269|PubMed:14981318,
CC       ECO:0000269|PubMed:9804162}.
CC   -!- INDUCTION: By salt, cold and drought stress.
CC       {ECO:0000269|PubMed:9804162}.
CC   -!- DOMAIN: Histidine-containing phosphotransfer domain (HPt) contains an
CC       active histidine that mediates the phosphotransfer.
CC   -!- PTM: Two-component system major event consists of a His-to-Asp
CC       phosphorelay between a sensor histidine kinase (HK) and a response
CC       regulator (RR). In plants, the His-to-Asp phosphorelay involves an
CC       additional intermediate named Histidine-containing phosphotransfer
CC       protein (HPt). This multistep phosphorelay consists of a His-Asp-His-
CC       Asp sequential transfer of a phosphate group between first an His and
CC       an Asp of the HK protein, followed by the transfer to a conserved His
CC       of the HPt protein and finally the transfer to an Asp in the receiver
CC       domain of the RR protein.
CC   -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC       {ECO:0000305}.
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DR   EMBL; AB012568; BAA37110.1; -; mRNA.
DR   EMBL; AB015142; BAA36336.1; -; mRNA.
DR   EMBL; AP001309; BAB02580.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE77571.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE77572.1; -; Genomic_DNA.
DR   EMBL; AY064148; AAL36054.1; -; mRNA.
DR   EMBL; AY097403; AAM19919.1; -; mRNA.
DR   RefSeq; NP_189581.1; NM_113860.4. [Q9ZNV8-1]
DR   RefSeq; NP_850649.1; NM_180318.2. [Q9ZNV8-2]
DR   PDB; 4PAC; X-ray; 2.53 A; A=1-156.
DR   PDBsum; 4PAC; -.
DR   AlphaFoldDB; Q9ZNV8; -.
DR   SMR; Q9ZNV8; -.
DR   BioGRID; 7922; 43.
DR   IntAct; Q9ZNV8; 46.
DR   STRING; 3702.AT3G29350.1; -.
DR   iPTMnet; Q9ZNV8; -.
DR   PaxDb; Q9ZNV8; -.
DR   PRIDE; Q9ZNV8; -.
DR   ProteomicsDB; 245060; -. [Q9ZNV8-1]
DR   DNASU; 822593; -.
DR   EnsemblPlants; AT3G29350.1; AT3G29350.1; AT3G29350. [Q9ZNV8-1]
DR   EnsemblPlants; AT3G29350.2; AT3G29350.2; AT3G29350. [Q9ZNV8-2]
DR   GeneID; 822593; -.
DR   Gramene; AT3G29350.1; AT3G29350.1; AT3G29350. [Q9ZNV8-1]
DR   Gramene; AT3G29350.2; AT3G29350.2; AT3G29350. [Q9ZNV8-2]
DR   KEGG; ath:AT3G29350; -.
DR   Araport; AT3G29350; -.
DR   TAIR; locus:2093817; AT3G29350.
DR   eggNOG; KOG4747; Eukaryota.
DR   HOGENOM; CLU_111777_1_0_1; -.
DR   InParanoid; Q9ZNV8; -.
DR   OMA; VCINFRN; -.
DR   PhylomeDB; Q9ZNV8; -.
DR   PRO; PR:Q9ZNV8; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9ZNV8; baseline and differential.
DR   Genevisible; Q9ZNV8; AT.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005829; C:cytosol; HDA:TAIR.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0009927; F:histidine phosphotransfer kinase activity; IDA:TAIR.
DR   GO; GO:0043424; F:protein histidine kinase binding; IPI:UniProtKB.
DR   GO; GO:0009557; P:antipodal cell differentiation; IGI:TAIR.
DR   GO; GO:0009736; P:cytokinin-activated signaling pathway; IMP:TAIR.
DR   GO; GO:0009560; P:embryo sac egg cell differentiation; IGI:TAIR.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IDA:TAIR.
DR   GO; GO:0016310; P:phosphorylation; IBA:GO_Central.
DR   CDD; cd00088; HPT; 1.
DR   Gene3D; 1.20.120.160; -; 1.
DR   InterPro; IPR045871; AHP1-5/YPD1.
DR   InterPro; IPR036641; HPT_dom_sf.
DR   InterPro; IPR008207; Sig_transdc_His_kin_Hpt_dom.
DR   PANTHER; PTHR28242; PTHR28242; 1.
DR   Pfam; PF01627; Hpt; 1.
DR   SUPFAM; SSF47226; SSF47226; 1.
DR   PROSITE; PS50894; HPT; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; Alternative splicing;
KW   Cytokinin signaling pathway; Cytoplasm; Nucleus; Phosphoprotein;
KW   Reference proteome; Stress response; Two-component regulatory system.
FT   CHAIN           1..156
FT                   /note="Histidine-containing phosphotransfer protein 2"
FT                   /id="PRO_0000074928"
FT   DOMAIN          40..147
FT                   /note="HPt"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00110"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0007744|PubMed:22223895"
FT   MOD_RES         82
FT                   /note="Phosphohistidine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00110"
FT   VAR_SEQ         115..156
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018143"
FT   HELIX           2..21
FT                   /evidence="ECO:0007829|PDB:4PAC"
FT   HELIX           27..33
FT                   /evidence="ECO:0007829|PDB:4PAC"
FT   HELIX           43..66
FT                   /evidence="ECO:0007829|PDB:4PAC"
FT   STRAND          69..71
FT                   /evidence="ECO:0007829|PDB:4PAC"
FT   HELIX           74..91
FT                   /evidence="ECO:0007829|PDB:4PAC"
FT   HELIX           94..108
FT                   /evidence="ECO:0007829|PDB:4PAC"
FT   HELIX           112..144
FT                   /evidence="ECO:0007829|PDB:4PAC"
FT   TURN            145..147
FT                   /evidence="ECO:0007829|PDB:4PAC"
SQ   SEQUENCE   156 AA;  17476 MW;  9A8FA1184F0E4462 CRC64;
     MDALIAQLQR QFRDYTISLY QQGFLDDQFT ELKKLQDDGS PDFVSEVLSL FFEDCVKLIS
     NMARALDTTG TVDFSQVGAS VHQLKGSSSS VGAKRVKTLC VSFKECCEAK NYEGCVRCLQ
     QVDIEYKALK TKLQDMFNLE KQIIQAGGIV PQVDIN
 
 
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