AHP4_ARATH
ID AHP4_ARATH Reviewed; 127 AA.
AC Q9LU15; O04321;
DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Histidine-containing phosphotransfer protein 4;
GN Name=AHP4; OrderedLocusNames=At3g16360; ORFNames=MYA6.16, T2O4.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=11193423; DOI=10.1271/bbb.64.2486;
RA Suzuki T., Sakurai K., Imamura A., Nakamura A., Ueguchi C., Mizuno T.;
RT "Compilation and characterization of histidine-containing
RT phosphotransmitters implicated in His-to-Asp phosphorelay in plants: AHP
RT signal transducers of Arabidopsis thaliana.";
RL Biosci. Biotechnol. Biochem. 64:2486-2489(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12068096; DOI=10.1104/pp.005504;
RA Hwang I., Chen H.-C., Sheen J.;
RT "Two-component signal transduction pathways in Arabidopsis.";
RL Plant Physiol. 129:500-515(2002).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND INTERACTION.
RX PubMed=14981318; DOI=10.1271/bbb.68.462;
RA Tanaka Y., Suzuki T., Yamashino T., Mizuno T.;
RT "Comparative studies of the AHP histidine-containing phosphotransmitters
RT implicated in His-to-Asp phosphorelay in Arabidopsis thaliana.";
RL Biosci. Biotechnol. Biochem. 68:462-465(2004).
CC -!- FUNCTION: Functions as two-component phosphorelay mediators between
CC cytokinin sensor histidine kinases and response regulators (B-type
CC ARRs). Plays an important role in propagating cytokinin signal
CC transduction through the multistep His-to-Asp phosphorelay.
CC {ECO:0000269|PubMed:11193423, ECO:0000269|PubMed:12068096,
CC ECO:0000269|PubMed:14981318}.
CC -!- SUBUNIT: Interacts with the B-type response regulators ARR1 and ARR2.
CC {ECO:0000269|PubMed:14981318}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}. Nucleus
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9LU15-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in aerial parts of the
CC plant. {ECO:0000269|PubMed:14981318}.
CC -!- DOMAIN: Histidine-containing phosphotransfer domain (HPt) contains an
CC active histidine that mediates the phosphotransfer.
CC -!- PTM: Two-component system major event consists of a His-to-Asp
CC phosphorelay between a sensor histidine kinase (HK) and a response
CC regulator (RR). In plants, the His-to-Asp phosphorelay involves an
CC additional intermediate named Histidine-containing phosphotransfer
CC protein (HPt). This multistep phosphorelay consists of a His-Asp-His-
CC Asp sequential transfer of a phosphate group between first an His and
CC an Asp of the HK protein, followed by the transfer to a conserved His
CC of the HPt protein and finally the transfer to an Asp in the receiver
CC domain of the RR protein.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB63642.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAB01275.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB041766; BAA94763.1; -; mRNA.
DR EMBL; AB023046; BAB01275.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC001645; AAB63642.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75802.1; -; Genomic_DNA.
DR RefSeq; NP_566544.1; NM_112507.1. [Q9LU15-1]
DR AlphaFoldDB; Q9LU15; -.
DR SMR; Q9LU15; -.
DR BioGRID; 6217; 10.
DR IntAct; Q9LU15; 9.
DR STRING; 3702.AT3G16360.2; -.
DR PaxDb; Q9LU15; -.
DR PRIDE; Q9LU15; -.
DR ProteomicsDB; 244995; -. [Q9LU15-1]
DR EnsemblPlants; AT3G16360.1; AT3G16360.1; AT3G16360. [Q9LU15-1]
DR GeneID; 820883; -.
DR Gramene; AT3G16360.1; AT3G16360.1; AT3G16360. [Q9LU15-1]
DR KEGG; ath:AT3G16360; -.
DR Araport; AT3G16360; -.
DR eggNOG; KOG4747; Eukaryota.
DR HOGENOM; CLU_111777_1_0_1; -.
DR InParanoid; Q9LU15; -.
DR PhylomeDB; Q9LU15; -.
DR PRO; PR:Q9LU15; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LU15; baseline and differential.
DR Genevisible; Q9LU15; AT.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0009927; F:histidine phosphotransfer kinase activity; IBA:GO_Central.
DR GO; GO:0043424; F:protein histidine kinase binding; IBA:GO_Central.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IBA:GO_Central.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IBA:GO_Central.
DR Gene3D; 1.20.120.160; -; 1.
DR InterPro; IPR045871; AHP1-5/YPD1.
DR InterPro; IPR036641; HPT_dom_sf.
DR InterPro; IPR008207; Sig_transdc_His_kin_Hpt_dom.
DR PANTHER; PTHR28242; PTHR28242; 1.
DR Pfam; PF01627; Hpt; 1.
DR SUPFAM; SSF47226; SSF47226; 1.
DR PROSITE; PS50894; HPT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytokinin signaling pathway; Cytoplasm; Nucleus;
KW Phosphoprotein; Reference proteome; Two-component regulatory system.
FT CHAIN 1..127
FT /note="Histidine-containing phosphotransfer protein 4"
FT /id="PRO_0000074930"
FT DOMAIN 27..122
FT /note="HPt"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00110"
FT MOD_RES 68
FT /note="Phosphohistidine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00110"
SQ SEQUENCE 127 AA; 14669 MW; D03C095E55DF05B5 CRC64;
MTNIGKCMQG YLDEQFMELE ELQDDANPNF VEEVSALYFK DSARLINNID QALERGSFDF
NRLDSYMHQF KGSSTSIGAS KVKAECTTFR EYCRAGNAEG CLRTFQQLKK EHSTLRKKLE
HYFQASQ
