AHP5_ARATH
ID AHP5_ARATH Reviewed; 157 AA.
AC Q8L9T7; Q9LR80; Q9MAY1;
DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Histidine-containing phosphotransfer protein 5;
GN Name=AHP5; OrderedLocusNames=At1g03430; ORFNames=F21B7.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=11193423; DOI=10.1271/bbb.64.2486;
RA Suzuki T., Sakurai K., Imamura A., Nakamura A., Ueguchi C., Mizuno T.;
RT "Compilation and characterization of histidine-containing
RT phosphotransmitters implicated in His-to-Asp phosphorelay in plants: AHP
RT signal transducers of Arabidopsis thaliana.";
RL Biosci. Biotechnol. Biochem. 64:2486-2489(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH AHK4.
RX PubMed=11230563; DOI=10.1093/pcp/pce037;
RA Suzuki T., Miwa K., Ishikawa K., Yamada H., Aiba H., Mizuno T.;
RT "The Arabidopsis sensor His-kinase, AHk4, can respond to cytokinins.";
RL Plant Cell Physiol. 42:107-113(2001).
RN [6]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12068096; DOI=10.1104/pp.005504;
RA Hwang I., Chen H.-C., Sheen J.;
RT "Two-component signal transduction pathways in Arabidopsis.";
RL Plant Physiol. 129:500-515(2002).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND INTERACTION.
RX PubMed=14981318; DOI=10.1271/bbb.68.462;
RA Tanaka Y., Suzuki T., Yamashino T., Mizuno T.;
RT "Comparative studies of the AHP histidine-containing phosphotransmitters
RT implicated in His-to-Asp phosphorelay in Arabidopsis thaliana.";
RL Biosci. Biotechnol. Biochem. 68:462-465(2004).
RN [8]
RP INTERACTION WITH AHK2; AHK3 AND AHK4, AND MUTAGENESIS OF HIS-83.
RX PubMed=16965536; DOI=10.1111/j.1742-4658.2006.05467.x;
RA Dortay H., Mehnert N., Buerkle L., Schmuelling T., Heyl A.;
RT "Analysis of protein interactions within the cytokinin-signaling pathway of
RT Arabidopsis thaliana.";
RL FEBS J. 273:4631-4644(2006).
RN [9]
RP SUBCELLULAR LOCATION.
RX PubMed=18642946; DOI=10.1021/pr0703831;
RA Dortay H., Gruhn N., Pfeifer A., Schwerdtner M., Schmuelling T., Heyl A.;
RT "Toward an interaction map of the two-component signaling pathway of
RT Arabidopsis thaliana.";
RL J. Proteome Res. 7:3649-3660(2008).
RN [10]
RP INTERACTION WITH AHK5.
RX PubMed=23132142; DOI=10.1093/mp/sss126;
RA Bauer J., Reiss K., Veerabagu M., Heunemann M., Harter K., Stehle T.;
RT "Structure-function analysis of Arabidopsis thaliana histidine kinase AHK5
RT bound to its cognate phosphotransfer protein AHP1.";
RL Mol. Plant 6:959-970(2013).
CC -!- FUNCTION: Functions as two-component phosphorelay mediators between
CC cytokinin sensor histidine kinases and response regulators (B-type
CC ARRs). Plays an important role in propagating cytokinin signal
CC transduction through the multistep His-to-Asp phosphorelay.
CC {ECO:0000269|PubMed:11193423, ECO:0000269|PubMed:12068096,
CC ECO:0000269|PubMed:14981318}.
CC -!- SUBUNIT: Interacts with the B-type response regulators ARR1 and ARR2.
CC Binds to AHK2, AHK3, AHK4 and AHK5. {ECO:0000269|PubMed:11230563,
CC ECO:0000269|PubMed:14981318, ECO:0000269|PubMed:16965536,
CC ECO:0000269|PubMed:23132142}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:18642946}.
CC Nucleus {ECO:0000269|PubMed:18642946}.
CC -!- TISSUE SPECIFICITY: Expressed in the whole plant.
CC {ECO:0000269|PubMed:14981318}.
CC -!- DOMAIN: Histidine-containing phosphotransfer domain (HPt) contains an
CC active histidine that mediates the phosphotransfer.
CC -!- PTM: Two-component system major event consists of a His-to-Asp
CC phosphorelay between a sensor histidine kinase (HK) and a response
CC regulator (RR). In plants, the His-to-Asp phosphorelay involves an
CC additional intermediate named Histidine-containing phosphotransfer
CC protein (HPt). This multistep phosphorelay consists of a His-Asp-His-
CC Asp sequential transfer of a phosphate group between first an His and
CC an Asp of the HK protein, followed by the transfer to a conserved His
CC of the HPt protein and finally the transfer to an Asp in the receiver
CC domain of the RR protein.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF86510.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB041767; BAA94764.1; -; mRNA.
DR EMBL; AC002560; AAF86510.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27570.1; -; Genomic_DNA.
DR EMBL; AY088228; AAM65769.1; -; mRNA.
DR RefSeq; NP_563684.1; NM_100225.3.
DR AlphaFoldDB; Q8L9T7; -.
DR SMR; Q8L9T7; -.
DR BioGRID; 24741; 31.
DR IntAct; Q8L9T7; 14.
DR STRING; 3702.AT1G03430.1; -.
DR PaxDb; Q8L9T7; -.
DR PRIDE; Q8L9T7; -.
DR ProteomicsDB; 244837; -.
DR EnsemblPlants; AT1G03430.1; AT1G03430.1; AT1G03430.
DR GeneID; 839506; -.
DR Gramene; AT1G03430.1; AT1G03430.1; AT1G03430.
DR KEGG; ath:AT1G03430; -.
DR Araport; AT1G03430; -.
DR TAIR; locus:2020868; AT1G03430.
DR eggNOG; KOG4747; Eukaryota.
DR HOGENOM; CLU_111777_1_0_1; -.
DR InParanoid; Q8L9T7; -.
DR OMA; IPQVDIN; -.
DR OrthoDB; 1488145at2759; -.
DR PhylomeDB; Q8L9T7; -.
DR PRO; PR:Q8L9T7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8L9T7; baseline and differential.
DR Genevisible; Q8L9T7; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0009927; F:histidine phosphotransfer kinase activity; IDA:TAIR.
DR GO; GO:0043424; F:protein histidine kinase binding; IPI:UniProtKB.
DR GO; GO:0009557; P:antipodal cell differentiation; IGI:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IMP:TAIR.
DR GO; GO:0009560; P:embryo sac egg cell differentiation; IGI:TAIR.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IBA:GO_Central.
DR CDD; cd00088; HPT; 1.
DR Gene3D; 1.20.120.160; -; 1.
DR InterPro; IPR045871; AHP1-5/YPD1.
DR InterPro; IPR036641; HPT_dom_sf.
DR InterPro; IPR008207; Sig_transdc_His_kin_Hpt_dom.
DR PANTHER; PTHR28242; PTHR28242; 1.
DR Pfam; PF01627; Hpt; 1.
DR SUPFAM; SSF47226; SSF47226; 1.
DR PROSITE; PS50894; HPT; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytokinin signaling pathway; Cytoplasm; Nucleus;
KW Phosphoprotein; Reference proteome; Two-component regulatory system.
FT CHAIN 1..157
FT /note="Histidine-containing phosphotransfer protein 5"
FT /id="PRO_0000074931"
FT DOMAIN 41..148
FT /note="HPt"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00110"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9ZNV8"
FT MOD_RES 83
FT /note="Phosphohistidine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00110"
FT MUTAGEN 83
FT /note="H->K,A: Impaired AHK4 binding."
FT /evidence="ECO:0000269|PubMed:16965536"
SQ SEQUENCE 157 AA; 17925 MW; 928CD8C6A09CEE9F CRC64;
MNTIVVAQLQ RQFQDYIVSL YQQGFLDNQF SELRKLQDEG TPDFVAEVVS LFFDDCSKLI
NTMSISLERP DNVDFKQVDS GVHQLKGSSS SVGARRVKNV CISFKECCDV QNREGCLRCL
QQVDYEYKML KTKLQDLFNL EKQILQAGGT IPQVDIN
