ID   F16PC_CLOBA             Reviewed;         659 AA.
AC   B2V5F2;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Fructose-1,6-bisphosphatase class 3 {ECO:0000255|HAMAP-Rule:MF_01854};
DE            Short=FBPase class 3 {ECO:0000255|HAMAP-Rule:MF_01854};
DE            EC=3.1.3.11 {ECO:0000255|HAMAP-Rule:MF_01854};
DE   AltName: Full=D-fructose-1,6-bisphosphate 1-phosphohydrolase class 3 {ECO:0000255|HAMAP-Rule:MF_01854};
GN   Name=fbp {ECO:0000255|HAMAP-Rule:MF_01854}; OrderedLocusNames=CLH_2300;
OS   Clostridium botulinum (strain Alaska E43 / Type E3).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=508767;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Alaska E43 / Type E3;
RA   Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A.,
RA   Smith T.J., Sutton G., Brettin T.S.;
RT   "Complete genome sequence of Clostridium botulinum E3 str. Alaska E43.";
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-
CC         phosphate + phosphate; Xref=Rhea:RHEA:11064, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:32966, ChEBI:CHEBI:43474, ChEBI:CHEBI:57634; EC=3.1.3.11;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01854};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01854};
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC       {ECO:0000255|HAMAP-Rule:MF_01854}.
CC   -!- SIMILARITY: Belongs to the FBPase class 3 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01854}.
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DR   EMBL; CP001078; ACD52126.1; -; Genomic_DNA.
DR   AlphaFoldDB; B2V5F2; -.
DR   PRIDE; B2V5F2; -.
DR   KEGG; cbt:CLH_2300; -.
DR   HOGENOM; CLU_028392_2_0_9; -.
DR   OMA; AKMHKAI; -.
DR   UniPathway; UPA00138; -.
DR   GO; GO:0042132; F:fructose 1,6-bisphosphate 1-phosphatase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.60.21.10; -; 1.
DR   HAMAP; MF_01854; FBPase_class3; 1.
DR   InterPro; IPR009164; FBPtase_class3.
DR   InterPro; IPR029052; Metallo-depent_PP-like.
DR   Pfam; PF06874; FBPase_2; 1.
DR   PIRSF; PIRSF000906; FBPtase_Bacill; 1.
DR   SUPFAM; SSF56300; SSF56300; 2.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Hydrolase; Manganese.
FT   CHAIN           1..659
FT                   /note="Fructose-1,6-bisphosphatase class 3"
FT                   /id="PRO_0000363080"
SQ   SEQUENCE   659 AA;  76709 MW;  DE7E785B19D73097 CRC64;
     MNNNDVEDIK KLKYFRLLAK QYPNIALAAT EIINLEAILN LPKGTEHFLS DIHGEYEPFV
     HVLRNGSGVV KRKIEDLFCK SLLESDIKSL ATLIYYPEQK LDIVLKEERN IDDWYKITLN
     RLIEICRFCS SKYTRSKVRK ALPADFAYII EELLHEQFNG IDKEEYYDRI ITTIIDIGRA
     KEFIIALSKL IQRMIIDRLH IIGDIYDRGP RPDIIIDTLM EYHSVDIQWG NHDMLWMGAA
     AGVRTCVANV LRISTRYANL DLVEEIYGIN LLPLATFALK YYRDDPCESF IPKVKEDDPA
     ANQEVDLVSK MHKAITILQF KLEKEIIDRR PEFELEERLL LDKIDYEKGT IILNGTEYKL
     NDNNFPTIDP KDPYKLTEEE SVLIDKLVYS FVNSDKLQKH VRFLFSKGNM YLKFNSNLLF
     HGCIPLDDDG NLKSMWIQGE EYESKRLLEK FDSLSREGYF EKVGSEEKTY GMDIMWYLWT
     GPVSPLFGKK KMATFERYFI DDEIAHIEQK TGYYKLRDRE EMCDMILREF GLDPSESRII
     NGHVPVKKKN GESPIKANGK MIVIDGGFSK AYQKQTGIAG YTLIYNSYGL QLVSHEHFSS
     TEESIMKEKD ILSTTLVVEQ KLKRKTVEDT DIGKELQVQI KDLKELLLIY RKGIIKEIR