F170A_MOUSE
ID F170A_MOUSE Reviewed; 333 AA.
AC Q66LM6; B2RW56; Q66LM7;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Protein FAM170A;
DE AltName: Full=Zinc finger domain-containing protein;
DE AltName: Full=Zinc finger protein ZNFD;
GN Name=Fam170a; Synonyms=Gm93, Znfd;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J;
RA Huang C.Q., Liu S., Zhai Q.T.;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-213 AND SER-308, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=22231842; DOI=10.1007/s11010-011-1193-7;
RA Xu F., Wang W., Lei C., Liu Q., Qiu H., Muraleedharan V., Zhou B.,
RA Cheng H., Huang Z., Xu W., Li B., Wang M.;
RT "Activation of transcriptional activity of HSE by a novel mouse zinc finger
RT protein ZNFD specifically expressed in testis.";
RL Mol. Cell. Biochem. 363:409-417(2012).
CC -!- FUNCTION: Acts as a nuclear transcription factor that positively
CC regulates the expression of heat shock genes. Binds to heat shock
CC promoter elements (HSE). {ECO:0000269|PubMed:22231842}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22231842}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=b;
CC IsoId=Q66LM6-1; Sequence=Displayed;
CC Name=2; Synonyms=a;
CC IsoId=Q66LM6-2; Sequence=VSP_032558;
CC -!- TISSUE SPECIFICITY: Testis-specific. {ECO:0000269|PubMed:22231842}.
CC -!- DOMAIN: The N-terminus is necessary for nuclear localization. The C-
CC terminus is necessary for transcriptional activity (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the FAM170 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY692448; AAU04856.1; -; mRNA.
DR EMBL; AY692449; AAU04857.1; -; mRNA.
DR EMBL; BC147592; AAI47593.1; -; mRNA.
DR EMBL; BC147595; AAI47596.1; -; mRNA.
DR CCDS; CCDS29243.1; -. [Q66LM6-1]
DR RefSeq; NP_001004061.1; NM_001004061.1. [Q66LM6-1]
DR AlphaFoldDB; Q66LM6; -.
DR BioGRID; 230401; 2.
DR STRING; 10090.ENSMUSP00000035910; -.
DR iPTMnet; Q66LM6; -.
DR PhosphoSitePlus; Q66LM6; -.
DR PaxDb; Q66LM6; -.
DR PRIDE; Q66LM6; -.
DR ProteomicsDB; 267678; -. [Q66LM6-1]
DR ProteomicsDB; 267679; -. [Q66LM6-2]
DR Antibodypedia; 50043; 62 antibodies from 11 providers.
DR DNASU; 225497; -.
DR Ensembl; ENSMUST00000039121; ENSMUSP00000035910; ENSMUSG00000035420. [Q66LM6-1]
DR GeneID; 225497; -.
DR KEGG; mmu:225497; -.
DR UCSC; uc008ewx.1; mouse. [Q66LM6-1]
DR CTD; 340069; -.
DR MGI; MGI:2684939; Fam170a.
DR VEuPathDB; HostDB:ENSMUSG00000035420; -.
DR eggNOG; ENOG502TH7F; Eukaryota.
DR GeneTree; ENSGT00940000162220; -.
DR HOGENOM; CLU_062038_2_0_1; -.
DR InParanoid; Q66LM6; -.
DR OMA; MEEMTLP; -.
DR OrthoDB; 1142827at2759; -.
DR PhylomeDB; Q66LM6; -.
DR TreeFam; TF337124; -.
DR BioGRID-ORCS; 225497; 1 hit in 73 CRISPR screens.
DR PRO; PR:Q66LM6; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q66LM6; protein.
DR Bgee; ENSMUSG00000035420; Expressed in seminiferous tubule of testis and 3 other tissues.
DR ExpressionAtlas; Q66LM6; baseline and differential.
DR Genevisible; Q66LM6; MM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; TAS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009566; P:fertilization; IBA:GO_Central.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IDA:UniProtKB.
DR InterPro; IPR033376; FAM170A.
DR InterPro; IPR040879; Spt46-like.
DR PANTHER; PTHR33517; PTHR33517; 1.
DR PANTHER; PTHR33517:SF3; PTHR33517:SF3; 1.
DR Pfam; PF17734; Spt46; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..333
FT /note="Protein FAM170A"
FT /id="PRO_0000326112"
FT ZN_FING 224..248
FT /note="C2H2-type; degenerate"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 73..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 143..214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 267..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..24
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..197
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 283..300
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 316..333
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 213
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 308
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 19..67
FT /note="GISKSQEDISHPESTGVPKAQSPGVGEVSSASEYFSCVSSPQKLIHRSK ->
FT E (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_032558"
SQ SEQUENCE 333 AA; 37392 MW; 145BD399D6C10F21 CRC64;
MKRRQKRKHL EIEESKEAGI SKSQEDISHP ESTGVPKAQS PGVGEVSSAS EYFSCVSSPQ
KLIHRSKGTW KLLQDSSKPR SPLDQVPEGE ATTAPSQQAS SSCPSYKTCV SSLCMNKEER
GMKIYYMQVQ MKKGVAISWD TKETSESLEK QPRMEEATLP EGVWVGTPPS DVSTRNLLSD
SEPIGEEKEH EEKPESDSPP GSPAVEERPR AKTPDWLVTM ENGFRCMACC RVFATMESLQ
EHVQYGIREG FSCHVFHLTM AQLIGSMESE STQEEEEDHT EETEKPKEEK AEEQQPTEED
VGMKKPWSQC PGCVFDSPKD RRRRKDHCDN SGS
