F177A_HUMAN
ID F177A_HUMAN Reviewed; 213 AA.
AC Q8N128; Q68CT2;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Protein FAM177A1;
GN Name=FAM177A1; Synonyms=C14orf24;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Mesangial cell;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Endometrium;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Platelet;
RX PubMed=18088087; DOI=10.1021/pr0704130;
RA Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J.,
RA Schuetz C., Walter U., Gambaryan S., Sickmann A.;
RT "Phosphoproteome of resting human platelets.";
RL J. Proteome Res. 7:526-534(2008).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70 AND THR-71, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70 AND THR-71, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- INTERACTION:
CC Q8N128-2; Q13520: AQP6; NbExp=3; IntAct=EBI-12201693, EBI-13059134;
CC Q8N128-2; P55212: CASP6; NbExp=3; IntAct=EBI-12201693, EBI-718729;
CC Q8N128-2; Q9P2X0-2: DPM3; NbExp=3; IntAct=EBI-12201693, EBI-10962476;
CC Q8N128-2; Q9GZR5: ELOVL4; NbExp=3; IntAct=EBI-12201693, EBI-18535450;
CC Q8N128-2; Q969F0: FATE1; NbExp=3; IntAct=EBI-12201693, EBI-743099;
CC Q8N128-2; Q8N5M9: JAGN1; NbExp=3; IntAct=EBI-12201693, EBI-10266796;
CC Q8N128-2; P13473-2: LAMP2; NbExp=3; IntAct=EBI-12201693, EBI-21591415;
CC Q8N128-2; Q96AL5: PBX3; NbExp=3; IntAct=EBI-12201693, EBI-741171;
CC Q8N128-2; O95470: SGPL1; NbExp=3; IntAct=EBI-12201693, EBI-1046170;
CC Q8N128-2; Q9Y371: SH3GLB1; NbExp=3; IntAct=EBI-12201693, EBI-2623095;
CC Q8N128-2; Q9Y320: TMX2; NbExp=3; IntAct=EBI-12201693, EBI-6447886;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8N128-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N128-2; Sequence=VSP_038223;
CC -!- SIMILARITY: Belongs to the FAM177 family. {ECO:0000305}.
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DR EMBL; AK096173; BAC04716.1; -; mRNA.
DR EMBL; CR749796; CAH18656.1; -; mRNA.
DR EMBL; BC029559; AAH29559.1; -; mRNA.
DR CCDS; CCDS41944.1; -. [Q8N128-1]
DR CCDS; CCDS9653.2; -. [Q8N128-2]
DR RefSeq; NP_001072987.1; NM_001079519.1. [Q8N128-1]
DR RefSeq; NP_001275951.1; NM_001289022.1. [Q8N128-1]
DR RefSeq; NP_775878.2; NM_173607.3. [Q8N128-2]
DR AlphaFoldDB; Q8N128; -.
DR SMR; Q8N128; -.
DR BioGRID; 129627; 53.
DR IntAct; Q8N128; 35.
DR MINT; Q8N128; -.
DR STRING; 9606.ENSP00000280987; -.
DR iPTMnet; Q8N128; -.
DR PhosphoSitePlus; Q8N128; -.
DR SwissPalm; Q8N128; -.
DR BioMuta; FAM177A1; -.
DR DMDM; 46395903; -.
DR EPD; Q8N128; -.
DR jPOST; Q8N128; -.
DR MassIVE; Q8N128; -.
DR MaxQB; Q8N128; -.
DR PaxDb; Q8N128; -.
DR PeptideAtlas; Q8N128; -.
DR PRIDE; Q8N128; -.
DR ProteomicsDB; 71528; -. [Q8N128-1]
DR ProteomicsDB; 71529; -. [Q8N128-2]
DR Antibodypedia; 23180; 42 antibodies from 10 providers.
DR DNASU; 283635; -.
DR Ensembl; ENST00000280987.9; ENSP00000280987.4; ENSG00000151327.13. [Q8N128-2]
DR Ensembl; ENST00000382406.7; ENSP00000371843.3; ENSG00000151327.13. [Q8N128-1]
DR Ensembl; ENST00000396472.5; ENSP00000379734.1; ENSG00000151327.13. [Q8N128-1]
DR GeneID; 283635; -.
DR KEGG; hsa:283635; -.
DR MANE-Select; ENST00000280987.9; ENSP00000280987.4; NM_173607.5; NP_775878.2. [Q8N128-2]
DR UCSC; uc001wsp.4; human. [Q8N128-1]
DR CTD; 283635; -.
DR DisGeNET; 283635; -.
DR GeneCards; FAM177A1; -.
DR HGNC; HGNC:19829; FAM177A1.
DR HPA; ENSG00000151327; Low tissue specificity.
DR MIM; 619181; gene.
DR neXtProt; NX_Q8N128; -.
DR OpenTargets; ENSG00000151327; -.
DR PharmGKB; PA162387378; -.
DR VEuPathDB; HostDB:ENSG00000151327; -.
DR eggNOG; ENOG502RYW3; Eukaryota.
DR GeneTree; ENSGT00390000016736; -.
DR HOGENOM; CLU_081605_0_0_1; -.
DR InParanoid; Q8N128; -.
DR OMA; ITLYCAP; -.
DR OrthoDB; 1572938at2759; -.
DR PhylomeDB; Q8N128; -.
DR TreeFam; TF326916; -.
DR PathwayCommons; Q8N128; -.
DR SignaLink; Q8N128; -.
DR BioGRID-ORCS; 283635; 16 hits in 1080 CRISPR screens.
DR ChiTaRS; FAM177A1; human.
DR GenomeRNAi; 283635; -.
DR Pharos; Q8N128; Tdark.
DR PRO; PR:Q8N128; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q8N128; protein.
DR Bgee; ENSG00000151327; Expressed in calcaneal tendon and 179 other tissues.
DR ExpressionAtlas; Q8N128; baseline and differential.
DR Genevisible; Q8N128; HS.
DR InterPro; IPR028260; FAM177.
DR PANTHER; PTHR31206; PTHR31206; 1.
DR Pfam; PF14774; FAM177; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Coiled coil; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..213
FT /note="Protein FAM177A1"
FT /id="PRO_0000089885"
FT REGION 147..175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 136..173
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 70
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:19690332"
FT MOD_RES 71
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:19690332"
FT VAR_SEQ 1
FT /note="M -> MEVGLPAITLFLTSASSPVVATTM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_038223"
FT CONFLICT 22
FT /note="A -> V (in Ref. 2; CAH18656)"
FT /evidence="ECO:0000305"
FT CONFLICT 47
FT /note="I -> M (in Ref. 2; CAH18656)"
FT /evidence="ECO:0000305"
FT CONFLICT 56
FT /note="R -> I (in Ref. 2; CAH18656)"
FT /evidence="ECO:0000305"
FT CONFLICT 62
FT /note="S -> I (in Ref. 2; CAH18656)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 213 AA; 23757 MW; 5AA0FE85489C4D78 CRC64;
MDQEPVGGVE RGEAVAASGA AAAAAFGESA GQMSNERGFE NVELGVIGKK KKVPRRVIHF
VSGETMEEYS TDEDEVDGLE KKDVLPTVDP TKLTWGPYLW FYMLRAATST LSVCDFLGEK
IASVLGISTP KYQYAIDEYY RMKKEEEEEE EENRMSEEAE KQYQQNKLQT DSIVQTDQPE
TVISSSFVNV NFEMEGDSEV IMESKQNPVS VPP
