ID   F17_VACCW               Reviewed;         101 AA.
AC   P07396; Q76ZW4;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Phosphoprotein F17;
GN   OrderedLocusNames=VACWR056; ORFNames=F17R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3856886; DOI=10.1073/pnas.82.7.2096;
RA   Bertholet C., Drillien R., Wittek R.;
RT   "One hundred base pairs of 5' flanking sequence of a vaccinia virus late
RT   gene are sufficient to temporally regulate late transcription.";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:2096-2100(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=1920628; DOI=10.1128/jvi.65.11.6101-6110.1991;
RA   Zhang Y., Moss B.;
RT   "Vaccinia virus morphogenesis is interrupted when expression of the gene
RT   encoding an 11-kilodalton phosphorylated protein is prevented by the
RT   Escherichia coli lac repressor.";
RL   J. Virol. 65:6101-6110(1991).
RN   [4]
RP   REVIEW, AND SUBCELLULAR LOCATION.
RX   PubMed=16877059; DOI=10.1016/s0065-3527(06)66002-8;
RA   Condit R.C., Moussatche N., Traktman P.;
RT   "In a nutshell: structure and assembly of the vaccinia virion.";
RL   Adv. Virus Res. 66:31-124(2006).
RN   [5]
RP   FUNCTION, SUBUNIT, INTERACTION WITH PROTEIN A30, PHOSPHORYLATION AT SER-53
RP   AND SER-62, AND MUTAGENESIS OF SER-53 AND SER-62.
RX   PubMed=20392848; DOI=10.1128/jvi.00399-10;
RA   Wickramasekera N.T., Traktman P.;
RT   "Structure/Function analysis of the vaccinia virus F18 phosphoprotein, an
RT   abundant core component required for virion maturation and infectivity.";
RL   J. Virol. 84:6846-6860(2010).
RN   [6]
RP   FUNCTION, INTERACTION WITH HOST RICTOR AND RPTOR, AND MUTAGENESIS OF SER-53
RP   AND SER-62.
RX   PubMed=30078703; DOI=10.1016/j.cell.2018.06.053;
RA   Meade N., Furey C., Li H., Verma R., Chai Q., Rollins M.G., DiGiuseppe S.,
RA   Naghavi M.H., Walsh D.;
RT   "Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2
RT   Regulatory Circuit.";
RL   Cell 0:0-0(2018).
CC   -!- FUNCTION: Plays an essential role in virion assembly and morphogenesis
CC       (PubMed:20392848). Also plays a role in the inhibition of host immune
CC       response by dysregulating mTOR. Sequesters host RICTOR and RPTOR,
CC       thereby disrupting mTORC1 and mTORC2 crosstalk. In turn, blocks the
CC       host antiviral response in part through mTOR-dependent degradation of
CC       cGAS, the primary poxvirus sensor (PubMed:30078703).
CC       {ECO:0000269|PubMed:20392848, ECO:0000269|PubMed:30078703}.
CC   -!- SUBUNIT: Self-associates to form high molecular-weight forms
CC       (PubMed:20392848). Interacts with protein A30 (PubMed:20392848).
CC       Interacts with host RICTOR and RPTOR; these interactions disrupt the
CC       mTORC1 and mTORC2 crosstalk (PubMed:30078703).
CC       {ECO:0000269|PubMed:20392848, ECO:0000269|PubMed:30078703}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305|PubMed:16877059}. Note=Major
CC       component of the virion comprising about 10% of the virion mass.
CC       {ECO:0000305}.
CC   -!- PTM: Phosphorylated on two serines. While these phosphorylations do not
CC       play a role in virion assembly; they are essential for the interaction
CC       with host RICTOR and RPTOR. {ECO:0000269|PubMed:20392848,
CC       ECO:0000269|PubMed:30078703}.
CC   -!- MISCELLANEOUS: Originally annotated as the product of the F18R open
CC       reading frame (protein F18), it is now referred as protein F17 since
CC       there are only 17 open reading frames in the HindIII fragment.
CC       {ECO:0000269|PubMed:20392848}.
CC   -!- SIMILARITY: Belongs to the poxviridae F17 protein family.
CC       {ECO:0000305}.
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DR   EMBL; M11107; AAA48278.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89335.1; -; Genomic_DNA.
DR   PIR; A22955; WMVZ11.
DR   iPTMnet; P07396; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0039503; P:suppression by virus of host innate immune response; IDA:UniProtKB.
DR   GO; GO:0019082; P:viral protein processing; IEA:InterPro.
DR   InterPro; IPR006854; Phosphoprotein_F17.
DR   Pfam; PF04767; Pox_F17; 1.
DR   PIRSF; PIRSF003688; VAC_PP; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Host-virus interaction;
KW   Inhibition of host innate immune response by virus; Late protein;
KW   Phosphoprotein; Reference proteome; Viral immunoevasion; Virion.
FT   CHAIN           1..101
FT                   /note="Phosphoprotein F17"
FT                   /id="PRO_0000099521"
FT   REGION          51..73
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         53
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:20392848"
FT   MOD_RES         62
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:20392848"
FT   MUTAGEN         53
FT                   /note="S->A: About 50% loss of phosphorylation."
FT                   /evidence="ECO:0000269|PubMed:20392848"
FT   MUTAGEN         53
FT                   /note="S->A: Complete loss of phosphorylation and about 90%
FT                   loss of binding with host RICTOR and RPTOR; when associated
FT                   with A-62."
FT                   /evidence="ECO:0000269|PubMed:20392848,
FT                   ECO:0000269|PubMed:30078703"
FT   MUTAGEN         62
FT                   /note="S->A: About 50% loss of phosphorylation."
FT                   /evidence="ECO:0000269|PubMed:20392848"
FT   MUTAGEN         62
FT                   /note="S->A: Complete loss of phosphorylation and about 90%
FT                   loss of binding with host RICTOR and RPTOR; when associated
FT                   with A-53."
FT                   /evidence="ECO:0000269|PubMed:20392848,
FT                   ECO:0000269|PubMed:30078703"
FT   CONFLICT        73
FT                   /note="R -> G (in Ref. 2; AAO89335)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   101 AA;  11435 MW;  D9917F95B27FA3E0 CRC64;
     MNSHFASAHT PFYINTKEGR YLVLKAVKVC DVRTVECEGS KASCVLKVDK PSSPACERRP
     SSPSRCERMN NPRKQVPFMR TDMLQNMFAA NRDNVASRLL N