F186B_HUMAN
ID F186B_HUMAN Reviewed; 893 AA.
AC Q8IYM0; B4DZ15; Q8TCP7; Q9H0L3;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Protein FAM186B;
GN Name=FAM186B; Synonyms=C12orf25;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT MET-397.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 388-893 (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
CC -!- INTERACTION:
CC Q8IYM0; O75381: PEX14; NbExp=2; IntAct=EBI-6269645, EBI-594898;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8IYM0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8IYM0-2; Sequence=VSP_056992, VSP_056993;
CC -!- SIMILARITY: Belongs to the FAM186 family. {ECO:0000305}.
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DR EMBL; AL136748; CAB66682.1; -; mRNA.
DR EMBL; AK302702; BAG63927.1; -; mRNA.
DR EMBL; AC020612; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC035621; AAH35621.1; -; mRNA.
DR EMBL; AL713673; CAD28479.2; -; mRNA.
DR CCDS; CCDS8788.1; -. [Q8IYM0-1]
DR RefSeq; NP_115506.1; NM_032130.2. [Q8IYM0-1]
DR RefSeq; XP_006719688.1; XM_006719625.2. [Q8IYM0-1]
DR RefSeq; XP_011537098.1; XM_011538796.2. [Q8IYM0-1]
DR AlphaFoldDB; Q8IYM0; -.
DR SMR; Q8IYM0; -.
DR BioGRID; 123865; 6.
DR IntAct; Q8IYM0; 5.
DR MINT; Q8IYM0; -.
DR STRING; 9606.ENSP00000257894; -.
DR iPTMnet; Q8IYM0; -.
DR PhosphoSitePlus; Q8IYM0; -.
DR BioMuta; FAM186B; -.
DR DMDM; 158931119; -.
DR EPD; Q8IYM0; -.
DR jPOST; Q8IYM0; -.
DR MassIVE; Q8IYM0; -.
DR MaxQB; Q8IYM0; -.
DR PaxDb; Q8IYM0; -.
DR PeptideAtlas; Q8IYM0; -.
DR PRIDE; Q8IYM0; -.
DR ProteomicsDB; 5563; -.
DR ProteomicsDB; 71201; -. [Q8IYM0-1]
DR Antibodypedia; 49160; 22 antibodies from 11 providers.
DR DNASU; 84070; -.
DR Ensembl; ENST00000257894.2; ENSP00000257894.2; ENSG00000135436.8. [Q8IYM0-1]
DR GeneID; 84070; -.
DR KEGG; hsa:84070; -.
DR MANE-Select; ENST00000257894.2; ENSP00000257894.2; NM_032130.3; NP_115506.1.
DR UCSC; uc001ruo.4; human. [Q8IYM0-1]
DR CTD; 84070; -.
DR DisGeNET; 84070; -.
DR GeneCards; FAM186B; -.
DR HGNC; HGNC:25296; FAM186B.
DR HPA; ENSG00000135436; Tissue enriched (testis).
DR neXtProt; NX_Q8IYM0; -.
DR OpenTargets; ENSG00000135436; -.
DR PharmGKB; PA164719890; -.
DR VEuPathDB; HostDB:ENSG00000135436; -.
DR eggNOG; ENOG502S05D; Eukaryota.
DR GeneTree; ENSGT00940000162031; -.
DR HOGENOM; CLU_015516_0_0_1; -.
DR InParanoid; Q8IYM0; -.
DR OMA; CLCHKYI; -.
DR PhylomeDB; Q8IYM0; -.
DR TreeFam; TF338344; -.
DR PathwayCommons; Q8IYM0; -.
DR SignaLink; Q8IYM0; -.
DR BioGRID-ORCS; 84070; 17 hits in 1068 CRISPR screens.
DR GeneWiki; Protein_FAM186B; -.
DR GenomeRNAi; 84070; -.
DR Pharos; Q8IYM0; Tdark.
DR PRO; PR:Q8IYM0; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q8IYM0; protein.
DR Bgee; ENSG00000135436; Expressed in left testis and 128 other tissues.
DR ExpressionAtlas; Q8IYM0; baseline and differential.
DR Genevisible; Q8IYM0; HS.
DR GO; GO:0032991; C:protein-containing complex; IDA:LIFEdb.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Reference proteome.
FT CHAIN 1..893
FT /note="Protein FAM186B"
FT /id="PRO_0000252147"
FT REGION 177..207
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 327..376
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 537..557
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 574..611
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 806..827
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 303..331
FT /evidence="ECO:0000255"
FT COMPBIAS 343..362
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 578..608
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..90
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056992"
FT VAR_SEQ 846..893
FT /note="QQGKQMEAVWKTEVASSSYAIEKKTPASLPRDQLRGHPDIPRLLTLDV ->
FT LLALCLRGLVAELALVMEDFDLLHVL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056993"
FT VARIANT 397
FT /note="V -> M (in dbSNP:rs17853450)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_027777"
FT VARIANT 553
FT /note="E -> Q (in dbSNP:rs12299908)"
FT /id="VAR_027778"
SQ SEQUENCE 893 AA; 103722 MW; 4657286AEAFC812E CRC64;
MEKDDPPQLV TPTSVKAIIL RIEAAQLTRA QEDISTQLSD ILDNVNCVIN RFQEELGYDL
KENAKSQQRD PKGKKRFILL EKIASFSKDA MMKEKHLYDI LRWLGDWGDT LTYEIGPRKS
EEEAAALDEW IEVTEKVLPL SLIATKRGIE SLTALCSTLI EGQKKRSQVS KRTFWQGWQG
RSPQTSPSHP QPLSPEQMLQ DQHTMNTKAS EVTSMLQELL DSTMFSKGEV RAIRYMATVV
ENLNKALILQ HKENRSLETK YRHLQMQATK ELSSQRLHFQ QFMEVLESRR DALLKQVEIL
GGRYHDLLLM KQALEFQLKK AQNATGQAED LAEVSVDSPG PSERETLPRK ETVMEESQQE
PMKEEQLFSP LPPSPMAMIR DSGAIAAGHQ PLSTMTVRSR VADVFGSKDT ESLEPVLLPL
VDRRFPKKWE RPVAESLGHK DKDQEDYFQK GGLQIKFHCS KQLSLESSRQ VTSESQEEPW
EEEFGREMRR QLWLEEEEMW QQRQKKWALL EQEHQEKLRQ WNLEDLAREQ QRRWVQLEKE
QESPRREPEQ LGEDVERRIF TPTSRWRDLE KAELSLVPAP SRTQSAHQSR RPHLPMSPST
QQPALGKQRP MSSVEFTYRP RTRRVPTKPK KSASFPVTGT SIRRLTWPSL QISPANIKKK
VYHMDMEAQR KNLQLLSEES ELRLPHYLRS KALELTTTTM ELGALRLQYL CHKYIFYRRL
QSLRQEAINH VQIMKETEAS YKAQNLYIFL ENIDRLQSLR LQAWTDKQKG LEEKHRECLS
SMVTMFPKLQ LEWNVHLNIP EVTSPKPKKC KLPAASPRHI RPSGPTYKQP FLSRHRACVP
LQMARQQGKQ MEAVWKTEVA SSSYAIEKKT PASLPRDQLR GHPDIPRLLT LDV
