ID   F1_VACCW                Reviewed;         226 AA.
AC   P24356; Q80HY1;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 2.
DT   29-SEP-2021, entry version 69.
DE   RecName: Full=Protein F1;
GN   OrderedLocusNames=VACWR040; ORFNames=F1L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-207.
RX   PubMed=2219701; DOI=10.1016/0042-6822(90)90338-r;
RA   Roseman N.A., Slabaugh M.B.;
RT   "The vaccinia virus HindIII F fragment: nucleotide sequence of the left 6.2
RT   kb.";
RL   Virology 178:410-418(1990).
RN   [3]
RP   FUNCTION, INTERACTION WITH HOST BAK, AND SUBCELLULAR LOCATION.
RX   PubMed=16439990; DOI=10.1038/sj.cdd.4401853;
RA   Postigo A., Cross J.R., Downward J., Way M.;
RT   "Interaction of F1L with the BH3 domain of Bak is responsible for
RT   inhibiting vaccinia-induced apoptosis.";
RL   Cell Death Differ. 13:1651-1662(2006).
RN   [4]
RP   FUNCTION, INTERACTION WITH HOST NLRP1, AND REGION.
RX   PubMed=23603272; DOI=10.1073/pnas.1215995110;
RA   Gerlic M., Faustin B., Postigo A., Yu E.C., Proell M., Gombosuren N.,
RA   Krajewska M., Flynn R., Croft M., Way M., Satterthwait A., Liddington R.C.,
RA   Salek-Ardakani S., Matsuzawa S., Reed J.C.;
RT   "Vaccinia virus F1L protein promotes virulence by inhibiting inflammasome
RT   activation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:7808-7813(2013).
CC   -!- FUNCTION: Plays a role in evading host innate immune response by
CC       inhibiting host inflammasome activation. Interacts with and inhibits
CC       NLR-mediated interleukin-1 beta/IL1B production in infected cells. At
CC       the host mitochondria, interacts with the BH3 domain of host BAK and
CC       prevents BAK from binding active BAX. In turn, host apoptosis is
CC       inhibited. {ECO:0000269|PubMed:16439990, ECO:0000269|PubMed:23603272}.
CC   -!- SUBUNIT: Homodimer. Interacts with host pro-apoptotic protein BCL2L11
CC       (via BH3 domain). Interacts with host NLRP1 (PubMed:23603272).
CC       Interacts with host BAK (PubMed:16439990).
CC       {ECO:0000250|UniProtKB:O57173, ECO:0000269|PubMed:23603272}.
CC   -!- INTERACTION:
CC       P24356; Q16611: BAK1; Xeno; NbExp=6; IntAct=EBI-8041400, EBI-519866;
CC   -!- SUBCELLULAR LOCATION: Host mitochondrion {ECO:0000269|PubMed:16439990}.
CC   -!- SIMILARITY: Belongs to the poxviridae F1 protein family. {ECO:0000305}.
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DR   EMBL; AY243312; AAO89319.1; -; Genomic_DNA.
DR   EMBL; M34368; AAA48247.1; -; mRNA.
DR   PIR; I36213; I36213.
DR   RefSeq; YP_232922.1; NC_006998.1.
DR   SMR; P24356; -.
DR   BioGRID; 3508851; 2.
DR   IntAct; P24356; 1.
DR   MINT; P24356; -.
DR   MEROPS; I91.001; -.
DR   DNASU; 3707497; -.
DR   GeneID; 3707497; -.
DR   KEGG; vg:3707497; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0033650; C:host cell mitochondrion; IDA:UniProtKB.
DR   GO; GO:0032692; P:negative regulation of interleukin-1 production; IDA:UniProtKB.
DR   GO; GO:0019050; P:suppression by virus of host apoptotic process; IDA:UniProtKB.
DR   Gene3D; 1.10.437.10; -; 1.
DR   InterPro; IPR036834; Bcl-2-like_sf.
DR   InterPro; IPR011207; Orthopox_F1.
DR   InterPro; IPR021119; Poxvirus_F1/C10.
DR   Pfam; PF11099; M11L; 1.
DR   PIRSF; PIRSF015971; VAC_F1L; 1.
PE   1: Evidence at protein level;
KW   Host mitochondrion; Host-virus interaction;
KW   Inhibition of host apoptosis by viral BCL2-like protein;
KW   Modulation of host cell apoptosis by virus; Reference proteome.
FT   CHAIN           1..226
FT                   /note="Protein F1"
FT                   /id="PRO_0000099476"
FT   REGION          32..37
FT                   /note="Essential and sufficient to inhibit host NLRP1"
FT                   /evidence="ECO:0000269|PubMed:23603272"
SQ   SEQUENCE   226 AA;  26428 MW;  68DC7EB188D29ECA CRC64;
     MLSMFMCNNI VDYVDDIDNG IVQDIEDEAS NNVDHDYVYP LPENMVYRFD KSTNILDYLS
     TERDHVMMAV RYYMSKQRLD DLYRQLPTKT RSYIDIINIY CDKVSNDYNR DMNIMYDMAS
     TKSFTVYDIN NEVNTILMDN KGLGVRLATI SFITELGRRC MNPVETIKMF TLLSHTICDD
     YFVDYITDIS PPDNTIPNTS TREYLKLIGI TAIMFATYKT LKYMIG