F221B_MOUSE
ID F221B_MOUSE Reviewed; 487 AA.
AC Q8C627; Q0VD71; Q8BGP2;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Protein FAM221B;
GN Name=Fam221b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Hypothalamus, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8C627-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8C627-2; Sequence=VSP_029102, VSP_029103;
CC -!- SIMILARITY: Belongs to the FAM221 family. {ECO:0000305}.
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DR EMBL; AK038644; BAC30077.1; -; mRNA.
DR EMBL; AK076646; BAC36435.1; -; mRNA.
DR EMBL; AK076680; BAC36443.1; -; mRNA.
DR EMBL; AL732626; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC119804; AAI19805.1; -; mRNA.
DR EMBL; BC122881; AAI22882.1; -; mRNA.
DR CCDS; CCDS18107.1; -. [Q8C627-1]
DR RefSeq; NP_780726.1; NM_175517.3. [Q8C627-1]
DR AlphaFoldDB; Q8C627; -.
DR STRING; 10090.ENSMUSP00000057398; -.
DR iPTMnet; Q8C627; -.
DR PhosphoSitePlus; Q8C627; -.
DR PaxDb; Q8C627; -.
DR PRIDE; Q8C627; -.
DR ProteomicsDB; 271537; -. [Q8C627-1]
DR ProteomicsDB; 271538; -. [Q8C627-2]
DR Antibodypedia; 5672; 40 antibodies from 11 providers.
DR Ensembl; ENSMUST00000056474; ENSMUSP00000057398; ENSMUSG00000043633. [Q8C627-1]
DR GeneID; 242408; -.
DR KEGG; mmu:242408; -.
DR UCSC; uc008sqt.1; mouse. [Q8C627-1]
DR UCSC; uc008squ.1; mouse. [Q8C627-2]
DR CTD; 392307; -.
DR MGI; MGI:2441678; Fam221b.
DR VEuPathDB; HostDB:ENSMUSG00000043633; -.
DR eggNOG; ENOG502R7X0; Eukaryota.
DR GeneTree; ENSGT00770000120611; -.
DR HOGENOM; CLU_053868_1_0_1; -.
DR InParanoid; Q8C627; -.
DR OMA; CSCFESN; -.
DR OrthoDB; 973186at2759; -.
DR PhylomeDB; Q8C627; -.
DR TreeFam; TF328347; -.
DR BioGRID-ORCS; 242408; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Fam221b; mouse.
DR PRO; PR:Q8C627; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8C627; protein.
DR Bgee; ENSMUSG00000043633; Expressed in seminiferous tubule of testis and 79 other tissues.
DR Genevisible; Q8C627; MM.
DR InterPro; IPR026755; Fam221a/b.
DR PANTHER; PTHR31214; PTHR31214; 1.
DR Pfam; PF14753; FAM221; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..487
FT /note="Protein FAM221B"
FT /id="PRO_0000309265"
FT REGION 1..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 132..289
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..51
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 68..103
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 174..189
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 205..230
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 231..258
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 248
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q66HD8"
FT VAR_SEQ 334..342
FT /note="IYIGWRCPH -> EPRWPSRGL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029102"
FT VAR_SEQ 343..487
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029103"
FT CONFLICT 66
FT /note="T -> I (in Ref. 3; AAI19805/AAI22882)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 487 AA; 54292 MW; 76B7121F8DBD0E8B CRC64;
MEANKTTEGP QDTQDAKEQP SLEDTNAEDL YEATTHETPL QPSSSQKHFQ PPEIHLETPA
SLNPDTKGAE GLQDSSVSGT PPVKSSSSGL LSLPPQLSPQ SVTSLEKAIA EIPLEEFGYD
SPISKVKEEP LQLSPTANIP EYGSPISEVQ KESLEVSPTA NIPEYGSPIS EVQKEPLEVS
STANIPKYES PVSEVQKEPL EVSPTASIPE SSHELTSDKV PQTQVPESEH FPKHSFSESP
TQAKEDESTK EGEAGEDELA VGTSKIPAAK PGLRAKKKKE KRAGGVTSRP VVPAKRAELV
EMAKAVHREQ FDDQVNNLFQ WEKDSTLKAI QTGIYIGWRC PHYLWDCFRI GDESKCFCGH
LLREHQIISD LSVPCSVSQC RCLMFCFIPS RPEEVGEFWL KKRATFDPKA WRAQCRCKHT
HEEHAATGAH PCRHRGCFCP SFESNFLCVA CDRRWEEHET FFETGDTRRK GKRPYGANNI
KNWHRTY
