F227A_HUMAN
ID F227A_HUMAN Reviewed; 570 AA.
AC F5H4B4; B0QY52; B7Z7C6; Q5TG08;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Protein FAM227A;
GN Name=FAM227A;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10591208; DOI=10.1038/990031;
RA Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M.,
RA Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C.,
RA Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E.,
RA Bridgeman A.M., Buck D., Burgess J., Burrill W.D., Burton J., Carder C.,
RA Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G.,
RA Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V.,
RA Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M.,
RA Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A.,
RA Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C.,
RA Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E.,
RA Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F.,
RA Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M.,
RA Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A.,
RA Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D.,
RA Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y.,
RA Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S.,
RA Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E.,
RA Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L.,
RA Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L.,
RA Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N.,
RA Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A.,
RA Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L.,
RA Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P.,
RA Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P.,
RA Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q.,
RA Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J.,
RA Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J.,
RA Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D.,
RA Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T.,
RA Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P.,
RA Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K.,
RA Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R.,
RA Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L.,
RA McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J.,
RA Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E.,
RA Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P.,
RA Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y.,
RA Wright H.;
RT "The DNA sequence of human chromosome 22.";
RL Nature 402:489-495(1999).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-343; SER-348 AND SER-349, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F5H4B4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F5H4B4-2; Sequence=VSP_044139, VSP_044140;
CC -!- SIMILARITY: Belongs to the FAM227 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK301819; BAH13562.1; -; mRNA.
DR EMBL; AL021707; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL022320; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL035495; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001013669.1; NM_001013647.1. [F5H4B4-1]
DR RefSeq; NP_001277959.1; NM_001291030.1. [F5H4B4-2]
DR AlphaFoldDB; F5H4B4; -.
DR SMR; F5H4B4; -.
DR BioGRID; 571665; 1.
DR IntAct; F5H4B4; 1.
DR STRING; 9606.ENSP00000348086; -.
DR iPTMnet; F5H4B4; -.
DR PhosphoSitePlus; F5H4B4; -.
DR BioMuta; FAM227A; -.
DR DMDM; 212288106; -.
DR MassIVE; F5H4B4; -.
DR PaxDb; F5H4B4; -.
DR PeptideAtlas; F5H4B4; -.
DR PRIDE; F5H4B4; -.
DR Antibodypedia; 312; 7 antibodies from 6 providers.
DR DNASU; 646851; -.
DR Ensembl; ENST00000535113.7; ENSP00000445093.1; ENSG00000184949.18. [F5H4B4-1]
DR GeneID; 646851; -.
DR KEGG; hsa:646851; -.
DR MANE-Select; ENST00000535113.7; ENSP00000445093.1; NM_001013647.2; NP_001013669.1.
DR UCSC; uc062ege.1; human. [F5H4B4-1]
DR CTD; 646851; -.
DR DisGeNET; 646851; -.
DR GeneCards; FAM227A; -.
DR HGNC; HGNC:44197; FAM227A.
DR HPA; ENSG00000184949; Tissue enhanced (choroid).
DR neXtProt; NX_F5H4B4; -.
DR OpenTargets; ENSG00000184949; -.
DR VEuPathDB; HostDB:ENSG00000184949; -.
DR eggNOG; ENOG502QR30; Eukaryota.
DR GeneTree; ENSGT00940000162433; -.
DR InParanoid; F5H4B4; -.
DR OrthoDB; 1153514at2759; -.
DR PathwayCommons; F5H4B4; -.
DR SignaLink; F5H4B4; -.
DR BioGRID-ORCS; 646851; 4 hits in 283 CRISPR screens.
DR ChiTaRS; FAM227A; human.
DR GenomeRNAi; 646851; -.
DR Pharos; F5H4B4; Tdark.
DR PRO; PR:F5H4B4; -.
DR Proteomes; UP000005640; Chromosome 22.
DR RNAct; F5H4B4; protein.
DR Bgee; ENSG00000184949; Expressed in right uterine tube and 98 other tissues.
DR ExpressionAtlas; F5H4B4; baseline and differential.
DR InterPro; IPR029417; FAM227.
DR PANTHER; PTHR33560; PTHR33560; 1.
DR Pfam; PF14922; FWWh; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..570
FT /note="Protein FAM227A"
FT /id="PRO_0000419264"
FT REGION 87..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 336..374
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 519..570
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 339..374
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 539..564
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 343
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 348
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 349
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT VAR_SEQ 1..93
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_044139"
FT VAR_SEQ 94..98
FT /note="SPEDK -> MLFSW (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_044140"
FT CONFLICT 391
FT /note="V -> A (in Ref. 1; BAH13562)"
FT /evidence="ECO:0000305"
FT CONFLICT 410
FT /note="A -> T (in Ref. 1; BAH13562)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 570 AA; 66188 MW; AC7F64938A80E6F7 CRC64;
MNHFRKMEVI NLTTLPMIPV DEHLAVSLVA RNTMVKTVRK ELENNPPSCL IGSMHQVNQK
IADINLRTEP SANSLAIERF ELEKKALREK TRSSPEDKVK RQRKSQYSCK GSELRHARSS
VIKRKTADKN LLAELYQYSN FNSSKPNKLP NGVDFCDMVG NVVRAERDCL SGKHFCSGRE
LEKFLSSSSP RAIWLDSFWW IFHERYQPNK ELQNNLFDRI AQHYALLLFR VPKSHSEEAL
LKRLPSLLSK AVYTSFCCCF PQSWFDTHEF KSDICNTMSL WISGTYPSPQ SYDSWDYSEL
DPERFRREEL MLYRRRLTKG REFSLFAGKR AFSQKPAQSR KFYHPQSSSA NSPSEKTSSA
KQNSEKSLRM QNTAKEHHCQ TLVLKKPTQE VKRISEAREC ENMFPKKSCA ACKSPELTSN
LFNIYGKSPL IVYFLQNYAS LQQHGKNVLI VRREKTTSTP DCTPTYTDVI SETLCSMKKR
KDNLNQLYQH HWTEWNYFDK HLKELQDNFS REMKNIDPKA ADTKKANHMF IPPSAVNEES
PDKKTKEGKG GEGKRRETEV EHFFPLTSKP
