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F263_RAT
ID   F263_RAT                Reviewed;         555 AA.
AC   O35552; O35096; O35553; O35554; O35555; O35556; O35557; Q9QWQ5; Q9QWQ6;
DT   24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 158.
DE   RecName: Full=6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3;
DE            Short=6PF-2-K/Fru-2,6-P2ase 3;
DE            Short=PFK/FBPase 3;
DE   AltName: Full=6PF-2-K/Fru-2,6-P2ase brain-type isozyme;
DE   AltName: Full=RB2K;
DE   Includes:
DE     RecName: Full=6-phosphofructo-2-kinase;
DE              EC=2.7.1.105 {ECO:0000250|UniProtKB:Q16875};
DE   Includes:
DE     RecName: Full=Fructose-2,6-bisphosphatase;
DE              EC=3.1.3.46 {ECO:0000250|UniProtKB:Q16875};
GN   Name=Pfkfb3;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING.
RC   STRAIN=Sprague-Dawley; TISSUE=Brain;
RX   PubMed=9202288; DOI=10.1046/j.1471-4159.1997.69010001.x;
RA   Watanabe F., Sakai A., Furuya E.;
RT   "Novel isoforms of rat brain fructose 6-phosphate 2-kinase/fructose 2,6-
RT   bisphosphatase are generated by tissue-specific alternative splicing.";
RL   J. Neurochem. 69:1-9(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 61-369.
RC   STRAIN=Wistar; TISSUE=Placenta;
RA   Sakakibara R.;
RL   Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes both the synthesis and degradation of fructose 2,6-
CC       bisphosphate. {ECO:0000250|UniProtKB:Q16875}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-D-fructose 2,6-bisphosphate + H2O = beta-D-fructose 6-
CC         phosphate + phosphate; Xref=Rhea:RHEA:17289, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57634, ChEBI:CHEBI:58579; EC=3.1.3.46;
CC         Evidence={ECO:0000250|UniProtKB:Q16875};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17290;
CC         Evidence={ECO:0000250|UniProtKB:Q16875};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-
CC         bisphosphate + H(+); Xref=Rhea:RHEA:15653, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:57634, ChEBI:CHEBI:58579,
CC         ChEBI:CHEBI:456216; EC=2.7.1.105;
CC         Evidence={ECO:0000250|UniProtKB:Q16875};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15654;
CC         Evidence={ECO:0000250|UniProtKB:Q16875};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q16875}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=8;
CC       Name=1; Synonyms=RB2K1;
CC         IsoId=O35552-1; Sequence=Displayed;
CC       Name=2; Synonyms=RB2K2;
CC         IsoId=O35552-2; Sequence=VSP_004682;
CC       Name=3; Synonyms=RB2K3;
CC         IsoId=O35552-3; Sequence=VSP_004683;
CC       Name=4; Synonyms=RB2K4;
CC         IsoId=O35552-4; Sequence=VSP_004681;
CC       Name=5; Synonyms=RB2K5;
CC         IsoId=O35552-5; Sequence=VSP_004681, VSP_004682;
CC       Name=6; Synonyms=RB2K6;
CC         IsoId=O35552-6; Sequence=VSP_004681, VSP_004683;
CC       Name=7; Synonyms=RB2K7;
CC         IsoId=O35552-7; Sequence=VSP_004684;
CC       Name=8; Synonyms=RB2K8;
CC         IsoId=O35552-8; Sequence=VSP_004681, VSP_004684;
CC   -!- PTM: Phosphorylation by AMPK stimulates activity.
CC       {ECO:0000250|UniProtKB:Q16875}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the phosphoglycerate
CC       mutase family. {ECO:0000305}.
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DR   EMBL; D87240; BAA21749.1; -; mRNA.
DR   EMBL; D87241; BAA21750.1; -; mRNA.
DR   EMBL; D87242; BAA21751.1; -; mRNA.
DR   EMBL; D87243; BAA21752.1; -; mRNA.
DR   EMBL; D87244; BAA21753.1; -; mRNA.
DR   EMBL; D87245; BAA21754.1; -; mRNA.
DR   EMBL; D87246; BAA21755.1; -; mRNA.
DR   EMBL; D87247; BAA21756.1; -; mRNA.
DR   EMBL; AB006710; BAA22048.1; -; mRNA.
DR   RefSeq; NP_476476.1; NM_057135.1. [O35552-1]
DR   RefSeq; XP_006254254.1; XM_006254192.3. [O35552-3]
DR   RefSeq; XP_006254255.1; XM_006254193.3. [O35552-2]
DR   RefSeq; XP_006254258.1; XM_006254196.3. [O35552-4]
DR   RefSeq; XP_006254259.1; XM_006254197.3. [O35552-6]
DR   RefSeq; XP_006254260.1; XM_006254198.3. [O35552-5]
DR   AlphaFoldDB; O35552; -.
DR   SMR; O35552; -.
DR   ComplexPortal; CPX-2043; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 complex.
DR   IntAct; O35552; 2.
DR   STRING; 10116.ENSRNOP00000062965; -.
DR   iPTMnet; O35552; -.
DR   PhosphoSitePlus; O35552; -.
DR   jPOST; O35552; -.
DR   PaxDb; O35552; -.
DR   Ensembl; ENSRNOT00000025700; ENSRNOP00000025700; ENSRNOG00000018911. [O35552-2]
DR   Ensembl; ENSRNOT00000025731; ENSRNOP00000025731; ENSRNOG00000018911. [O35552-4]
DR   Ensembl; ENSRNOT00000051067; ENSRNOP00000040928; ENSRNOG00000018911. [O35552-3]
DR   Ensembl; ENSRNOT00000068354; ENSRNOP00000062965; ENSRNOG00000018911. [O35552-1]
DR   GeneID; 117276; -.
DR   KEGG; rno:117276; -.
DR   CTD; 5209; -.
DR   RGD; 619776; Pfkfb3.
DR   eggNOG; KOG0234; Eukaryota.
DR   GeneTree; ENSGT00950000182835; -.
DR   HOGENOM; CLU_006383_1_1_1; -.
DR   InParanoid; O35552; -.
DR   OMA; AEDTRIC; -.
DR   OrthoDB; 392001at2759; -.
DR   PhylomeDB; O35552; -.
DR   TreeFam; TF313541; -.
DR   BRENDA; 3.1.3.46; 5301.
DR   Reactome; R-RNO-9634600; Regulation of glycolysis by fructose 2,6-bisphosphate metabolism.
DR   PRO; PR:O35552; -.
DR   Proteomes; UP000002494; Chromosome 17.
DR   Bgee; ENSRNOG00000018911; Expressed in skeletal muscle tissue and 19 other tissues.
DR   ExpressionAtlas; O35552; baseline and differential.
DR   Genevisible; O35552; RN.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0003873; F:6-phosphofructo-2-kinase activity; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004331; F:fructose-2,6-bisphosphate 2-phosphatase activity; ISS:UniProtKB.
DR   GO; GO:0007420; P:brain development; IEP:RGD.
DR   GO; GO:0006003; P:fructose 2,6-bisphosphate metabolic process; IDA:RGD.
DR   GO; GO:0006000; P:fructose metabolic process; IEA:InterPro.
DR   GO; GO:0006110; P:regulation of glycolytic process; TAS:RGD.
DR   CDD; cd07067; HP_PGM_like; 1.
DR   Gene3D; 3.40.50.1240; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003094; 6Pfruct_kin.
DR   InterPro; IPR013079; 6Phosfructo_kin.
DR   InterPro; IPR013078; His_Pase_superF_clade-1.
DR   InterPro; IPR029033; His_PPase_superfam.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001345; PG/BPGM_mutase_AS.
DR   PANTHER; PTHR10606; PTHR10606; 1.
DR   Pfam; PF01591; 6PF2K; 1.
DR   Pfam; PF00300; His_Phos_1; 1.
DR   PRINTS; PR00991; 6PFRUCTKNASE.
DR   SMART; SM00855; PGAM; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF53254; SSF53254; 1.
DR   PROSITE; PS00175; PG_MUTASE; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; ATP-binding; Hydrolase; Kinase;
KW   Multifunctional enzyme; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Transferase.
FT   CHAIN           1..555
FT                   /note="6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
FT                   3"
FT                   /id="PRO_0000179969"
FT   REGION          1..245
FT                   /note="6-phosphofructo-2-kinase"
FT   REGION          246..555
FT                   /note="Fructose-2,6-bisphosphatase"
FT   REGION          475..504
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        125
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        155
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        254
FT                   /note="Tele-phosphohistidine intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   ACT_SITE        323
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         42..50
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         75
FT                   /ligand="beta-D-fructose 6-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57634"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         99
FT                   /ligand="beta-D-fructose 6-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57634"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         127
FT                   /ligand="beta-D-fructose 6-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57634"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         133
FT                   /ligand="beta-D-fructose 6-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57634"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         164..169
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         169
FT                   /ligand="beta-D-fructose 6-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57634"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         190
FT                   /ligand="beta-D-fructose 6-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57634"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         194
FT                   /ligand="beta-D-fructose 6-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57634"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         253
FT                   /ligand="beta-D-fructose 2,6-bisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58579"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         260
FT                   /ligand="beta-D-fructose 2,6-bisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58579"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         266
FT                   /ligand="beta-D-fructose 2,6-bisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58579"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         334
FT                   /ligand="beta-D-fructose 2,6-bisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58579"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         345..348
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P07953"
FT   BINDING         348
FT                   /ligand="beta-D-fructose 2,6-bisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58579"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         352
FT                   /ligand="beta-D-fructose 2,6-bisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58579"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         363
FT                   /ligand="beta-D-fructose 2,6-bisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58579"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         389..393
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P07953"
FT   BINDING         389
FT                   /ligand="beta-D-fructose 2,6-bisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58579"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   BINDING         393
FT                   /ligand="beta-D-fructose 2,6-bisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58579"
FT                   /evidence="ECO:0000250|UniProtKB:P07953"
FT   BINDING         425
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   SITE            253
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   SITE            260
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   SITE            388
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   MOD_RES         490
FT                   /note="Phosphoserine; by AMPK and PKA"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   MOD_RES         492
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   MOD_RES         496
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q16875"
FT   VAR_SEQ         448..476
FT                   /note="Missing (in isoform 4, isoform 5, isoform 6 and
FT                   isoform 8)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_004681"
FT   VAR_SEQ         535..555
FT                   /note="PLLGKACLRTVCHIFSKFSPY -> NMRSPRSGAESSQKH (in isoform
FT                   3 and isoform 6)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_004683"
FT   VAR_SEQ         543..555
FT                   /note="RTVCHIFSKFSPY -> T (in isoform 2 and isoform 5)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_004682"
FT   VAR_SEQ         543..555
FT                   /note="Missing (in isoform 7 and isoform 8)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_004684"
FT   CONFLICT        185
FT                   /note="D -> H (in Ref. 2; BAA22048)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        367
FT                   /note="V -> L (in Ref. 2; BAA22048)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   555 AA;  63676 MW;  45B2D090B44FCD8D CRC64;
     MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY ISKKLTRYLN
     WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK QCALAALRDV KSYLTKEGGQ
     IAVFDATNTT RERRHMILHF AKENDFKAFF IESVCDDPTV VASNIMEVKI SSPDYKDCNS
     AEAMDDFMKR INCYEASYQP LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM
     NIHVQPRTIY LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ
     LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ DKYYYRYPTG
     ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL DKSAEEMPYL KCPLHTVLKL
     TPVAYGCRVE SIYLNVESVS THRERSEAVK IQHFASVVRP SSYTELDFLS VESAKQDAKK
     GPNPLMRRNS VTPLASPEPT KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQPLLGKA
     CLRTVCHIFS KFSPY
 
 
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