F3GGT_ACTCC
ID F3GGT_ACTCC Reviewed; 457 AA.
AC A0A2R6QXF8; A0A3B8DWI7;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2019, sequence version 2.
DT 25-MAY-2022, entry version 13.
DE RecName: Full=Cyanidin 3-O-galactoside 2''-O-xylosyltransferase FGGT1 {ECO:0000305};
DE EC=2.4.2.50 {ECO:0000269|PubMed:21175894};
DE AltName: Full=Flavonoid 3-O-glycoside glycosyltransferase 1 {ECO:0000303|PubMed:21175894};
DE AltName: Full=UDP-xylose flavonoid O-glycosyltransferase 6B {ECO:0000305};
DE Short=AcUFGT6b {ECO:0000303|Ref.1};
GN Name=F3GGT1 {ECO:0000303|PubMed:21175894};
GN Synonyms=UFGT6B {ECO:0000303|Ref.1};
GN ORFNames=CEY00_Acc13879 {ECO:0000312|EMBL:PSS16444.1};
OS Actinidia chinensis var. chinensis (Chinese soft-hair kiwi).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Actinidiaceae; Actinidia.
OX NCBI_TaxID=1590841;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Yanfei L.;
RT "Identification and characterisation of AcUFGT6b, a xylosyltransferase
RT involved in anthocyanin modification in red-fleshed kiwifruit (Actinidia
RT chinensis).";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Red5;
RX PubMed=29661190; DOI=10.1186/s12864-018-4656-3;
RA Pilkington S.M., Crowhurst R., Hilario E., Nardozza S., Fraser L., Peng Y.,
RA Gunaseelan K., Simpson R., Tahir J., Deroles S.C., Templeton K., Luo Z.,
RA Davy M., Cheng C., McNeilage M., Scaglione D., Liu Y., Zhang Q., Datson P.,
RA De Silva N., Gardiner S.E., Bassett H., Chagne D., McCallum J.,
RA Dzierzon H., Deng C., Wang Y.Y., Barron L., Manako K., Bowen J.,
RA Foster T.M., Erridge Z.A., Tiffin H., Waite C.N., Davies K.M.,
RA Grierson E.P., Laing W.A., Kirk R., Chen X., Wood M., Montefiori M.,
RA Brummell D.A., Schwinn K.E., Catanach A., Fullerton C., Li D.,
RA Meiyalaghan S., Nieuwenhuizen N., Read N., Prakash R., Hunter D., Zhang H.,
RA McKenzie M., Knabel M., Harris A., Allan A.C., Gleave A., Chen A.,
RA Janssen B.J., Plunkett B., Ampomah-Dwamena C., Voogd C., Leif D.,
RA Lafferty D., Souleyre E.J.F., Varkonyi-Gasic E., Gambi F., Hanley J.,
RA Yao J.L., Cheung J., David K.M., Warren B., Marsh K., Snowden K.C.,
RA Lin-Wang K., Brian L., Martinez-Sanchez M., Wang M., Ileperuma N.,
RA Macnee N., Campin R., McAtee P., Drummond R.S.M., Espley R.V.,
RA Ireland H.S., Wu R., Atkinson R.G., Karunairetnam S., Bulley S.,
RA Chunkath S., Hanley Z., Storey R., Thrimawithana A.H., Thomson S.,
RA David C., Testolin R., Huang H., Hellens R.P., Schaffer R.J.;
RT "A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome
RT highlights the challenges associated with draft genomes and gene prediction
RT in plants.";
RL BMC Genomics 19:257-257(2018).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=21175894; DOI=10.1111/j.1365-313x.2010.04409.x;
RA Montefiori M., Espley R.V., Stevenson D., Cooney J., Datson P.M., Saiz A.,
RA Atkinson R.G., Hellens R.P., Allan A.C.;
RT "Identification and characterisation of F3GT1 and F3GGT1, two
RT glycosyltransferases responsible for anthocyanin biosynthesis in red-
RT fleshed kiwifruit (Actinidia chinensis).";
RL Plant J. 65:106-118(2011).
CC -!- FUNCTION: Xylosyltransferase involved in anthocyanin biosynthesis by
CC catalyzing the xylosylation of cyanidin 3-O-galactoside to form
CC cyanidin 3-O-[2-O-(-xylosyl)-galactoside] (PubMed:21175894). Required
CC for the accumulation of anthocyanin in red-fleshed kiwifruit varieties
CC (PubMed:21175894). {ECO:0000269|PubMed:21175894}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cyanidin 3-O-beta-D-galactoside + UDP-alpha-D-xylose =
CC cyanidin 3-O-[beta-D-xylosyl-(1->2)-beta-D-galactoside] + H(+) + UDP;
CC Xref=Rhea:RHEA:35435, ChEBI:CHEBI:15378, ChEBI:CHEBI:57632,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:71516, ChEBI:CHEBI:77935; EC=2.4.2.50;
CC Evidence={ECO:0000269|PubMed:21175894};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35436;
CC Evidence={ECO:0000269|PubMed:21175894};
CC -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in ovaries.
CC {ECO:0000269|PubMed:21175894}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the inner and outer pericarps of
CC developing fruit at 20 days after flowering (DAF) (PubMed:21175894).
CC Low expression detected at 49 DAF and gradually decreases up to 126 DAF
CC (PubMed:21175894). {ECO:0000269|PubMed:21175894}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000255, ECO:0000255|RuleBase:RU003718}.
CC -!- SEQUENCE CAUTION:
CC Sequence=PSS16444.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; MH817045; AYJ72754.1; -; mRNA.
DR EMBL; NKQK01000012; PSS16444.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; A0A2R6QXF8; -.
DR SMR; A0A2R6QXF8; -.
DR STRING; 1590841.A0A2R6QXF8; -.
DR EnsemblPlants; PSS16444; PSS16444; CEY00_Acc13879.
DR Gramene; PSS16444; PSS16444; CEY00_Acc13879.
DR UniPathway; UPA00009; -.
DR Proteomes; UP000241394; Chromosome lg12.
DR GO; GO:0035252; F:UDP-xylosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IDA:UniProtKB.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Flavonoid biosynthesis; Glycosyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..457
FT /note="Cyanidin 3-O-galactoside 2''-O-xylosyltransferase
FT FGGT1"
FT /id="PRO_0000448079"
FT CONFLICT 104
FT /note="F -> L (in Ref. 1; AYJ72754)"
FT /evidence="ECO:0000305"
FT CONFLICT 165
FT /note="T -> P (in Ref. 1; AYJ72754)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="L -> I (in Ref. 1; AYJ72754)"
FT /evidence="ECO:0000305"
FT CONFLICT 172..173
FT /note="SS -> FL (in Ref. 1; AYJ72754)"
FT /evidence="ECO:0000305"
FT CONFLICT 227
FT /note="F -> Y (in Ref. 1; AYJ72754)"
FT /evidence="ECO:0000305"
FT CONFLICT 233
FT /note="E -> G (in Ref. 1; AYJ72754)"
FT /evidence="ECO:0000305"
FT CONFLICT 264
FT /note="D -> A (in Ref. 1; AYJ72754)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 457 AA; 51044 MW; 93854598D5926855 CRC64;
MGAPTFHIAM YPWFALGHLT PFLHLSNKLA RKGHKISFLI PTKTQQKLEP FNLHPDLITF
IPVTVPHVDG LPLGAETTSD VPYPLQTLLM TAMDRTEKYV EDVFLGLKVD VVFFDFTHWM
PSVAKRLGIK SVNYCIISPA TIGYTMSPAR QLQGRELTEA DLMVTPLGYP DSSIRLRTHE
ARAFAARRVM KFGGDTRFCD RNFISFSECD AMGFKTCREI EGPYCDFLES QFEKPVLLSG
PVIPEPPTSP LEEIWAKWLG GFRDGSVIYC AFGSECTLKM NQFQELLLGL VLTGMPFLAV
LKPPIGAKSV EEALPEKFET SVEGRGVVHE GWVQQQLILE HPSVGCFITH CGSGSLSEAL
FNKCQLVLLP NVGDQIINAR MMSQNLKVGV EVEKGEEDGL FTGESVCRAV RDAMEEGSEV
AKEVRDNHAK MREFLLNKDL ESSYIDNFNK KLQDLLG
