F3GT1_ACTCC
ID F3GT1_ACTCC Reviewed; 456 AA.
AC A0A2R6Q8R5; A0A3B8DHU1; E5D2U2;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2018, sequence version 1.
DT 25-MAY-2022, entry version 16.
DE RecName: Full=Anthocyanidin 3-O-galactosyltransferase F3GT1 {ECO:0000305};
DE EC=2.4.1.294 {ECO:0000269|PubMed:21175894, ECO:0000269|PubMed:29057484};
DE AltName: Full=Flavonoid 3-O-glycosyltransferase 1 {ECO:0000303|PubMed:21175894};
DE AltName: Full=UDP-galactose flavonoid 3-O-galactosyltransferase 3A {ECO:0000303|PubMed:29057484};
DE Short=AcUFGT3a {ECO:0000303|PubMed:29057484};
GN Name=F3GT1 {ECO:0000303|PubMed:21175894};
GN Synonyms=UFGT3A {ECO:0000303|PubMed:29057484};
GN ORFNames=CEY00_Acc20131 {ECO:0000312|EMBL:PSS04285.1};
OS Actinidia chinensis var. chinensis (Chinese soft-hair kiwi).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Actinidiaceae; Actinidia.
OX NCBI_TaxID=1590841;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=21175894; DOI=10.1111/j.1365-313x.2010.04409.x;
RA Montefiori M., Espley R.V., Stevenson D., Cooney J., Datson P.M., Saiz A.,
RA Atkinson R.G., Hellens R.P., Allan A.C.;
RT "Identification and characterisation of F3GT1 and F3GGT1, two
RT glycosyltransferases responsible for anthocyanin biosynthesis in red-
RT fleshed kiwifruit (Actinidia chinensis).";
RL Plant J. 65:106-118(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=29057484; DOI=10.1111/ppl.12655;
RA Liu Y., Zhou B., Qi Y., Liu C., Liu Z., Ren X.;
RT "Biochemical and functional characterization of AcUFGT3a, a
RT galactosyltransferase involved in anthocyanin biosynthesis in the red-
RT fleshed kiwifruit (Actinidia chinensis).";
RL Physiol. Plantarum 162:409-426(2018).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Red5;
RX PubMed=29661190; DOI=10.1186/s12864-018-4656-3;
RA Pilkington S.M., Crowhurst R., Hilario E., Nardozza S., Fraser L., Peng Y.,
RA Gunaseelan K., Simpson R., Tahir J., Deroles S.C., Templeton K., Luo Z.,
RA Davy M., Cheng C., McNeilage M., Scaglione D., Liu Y., Zhang Q., Datson P.,
RA De Silva N., Gardiner S.E., Bassett H., Chagne D., McCallum J.,
RA Dzierzon H., Deng C., Wang Y.Y., Barron L., Manako K., Bowen J.,
RA Foster T.M., Erridge Z.A., Tiffin H., Waite C.N., Davies K.M.,
RA Grierson E.P., Laing W.A., Kirk R., Chen X., Wood M., Montefiori M.,
RA Brummell D.A., Schwinn K.E., Catanach A., Fullerton C., Li D.,
RA Meiyalaghan S., Nieuwenhuizen N., Read N., Prakash R., Hunter D., Zhang H.,
RA McKenzie M., Knabel M., Harris A., Allan A.C., Gleave A., Chen A.,
RA Janssen B.J., Plunkett B., Ampomah-Dwamena C., Voogd C., Leif D.,
RA Lafferty D., Souleyre E.J.F., Varkonyi-Gasic E., Gambi F., Hanley J.,
RA Yao J.L., Cheung J., David K.M., Warren B., Marsh K., Snowden K.C.,
RA Lin-Wang K., Brian L., Martinez-Sanchez M., Wang M., Ileperuma N.,
RA Macnee N., Campin R., McAtee P., Drummond R.S.M., Espley R.V.,
RA Ireland H.S., Wu R., Atkinson R.G., Karunairetnam S., Bulley S.,
RA Chunkath S., Hanley Z., Storey R., Thrimawithana A.H., Thomson S.,
RA David C., Testolin R., Huang H., Hellens R.P., Schaffer R.J.;
RT "A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome
RT highlights the challenges associated with draft genomes and gene prediction
RT in plants.";
RL BMC Genomics 19:257-257(2018).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=25143057; DOI=10.1111/ppl.12263;
RA Man Y.P., Wang Y.C., Li Z.Z., Jiang Z.W., Yang H.L., Gong J.J., He S.S.,
RA Wu S.Q., Yang Z.Q., Zheng J., Wang Z.Y.;
RT "High-temperature inhibition of biosynthesis and transportation of
RT anthocyanins results in the poor red coloration in red-fleshed Actinidia
RT chinensis.";
RL Physiol. Plantarum 153:565-583(2015).
RN [5]
RP FUNCTION.
RX PubMed=30912865; DOI=10.1111/tpj.14330;
RA Wang L., Tang W., Hu Y., Zhang Y., Sun J., Guo X., Lu H., Yang Y., Fang C.,
RA Niu X., Yue J., Fei Z., Liu Y.;
RT "A MYB/bHLH complex regulates tissue-specific anthocyanin biosynthesis in
RT the inner pericarp of red-centered kiwifruit Actinidia chinensis cv.
RT Hongyang.";
RL Plant J. 99:359-378(2019).
CC -!- FUNCTION: Involved in anthocyanin biosynthesis by catalyzing the
CC galactosylation of cyanidin (PubMed:21175894, PubMed:29057484,
CC PubMed:30912865). Required for the accumulation of anthocyanin in red-
CC fleshed kiwifruit varieties (PubMed:21175894, PubMed:29057484,
CC PubMed:30912865). Seems to be the key enzyme regulating the
CC accumulation of anthocyanin in red-fleshed kiwi fruits
CC (PubMed:21175894, PubMed:29057484, PubMed:30912865).
CC {ECO:0000269|PubMed:21175894, ECO:0000269|PubMed:29057484,
CC ECO:0000269|PubMed:30912865}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cyanidin + UDP-alpha-D-galactose = cyanidin 3-O-beta-D-
CC galactoside + H(+) + UDP; Xref=Rhea:RHEA:35631, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:66914, ChEBI:CHEBI:71682,
CC ChEBI:CHEBI:77935; EC=2.4.1.294;
CC Evidence={ECO:0000269|PubMed:21175894, ECO:0000269|PubMed:29057484};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35632;
CC Evidence={ECO:0000269|PubMed:21175894, ECO:0000269|PubMed:29057484};
CC -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in stems and leaves
CC (PubMed:25143057). Expressed in ovaries (PubMed:21175894).
CC {ECO:0000269|PubMed:21175894, ECO:0000269|PubMed:25143057}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the inner and outer pericarps of
CC developing fruit at 20 days after flowering (DAF) (PubMed:21175894).
CC Expressed only in the inner pericarp from 86 to 126 DAF
CC (PubMed:21175894). {ECO:0000269|PubMed:21175894}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; GU079683; ADC34700.1; -; mRNA.
DR EMBL; MH817049; AYJ72756.1; -; mRNA.
DR EMBL; NKQK01000018; PSS04285.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2R6Q8R5; -.
DR SMR; A0A2R6Q8R5; -.
DR STRING; 1590841.A0A2R6Q8R5; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR EnsemblPlants; PSS04285; PSS04285; CEY00_Acc20131.
DR Gramene; PSS04285; PSS04285; CEY00_Acc20131.
DR OMA; FFWFAKE; -.
DR BRENDA; 2.4.1.294; 17373.
DR UniPathway; UPA00009; -.
DR Proteomes; UP000241394; Chromosome lg18.
DR GO; GO:0102454; F:cyanidin 3-O-galactosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IDA:UniProtKB.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Flavonoid biosynthesis; Glycosyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..456
FT /note="Anthocyanidin 3-O-galactosyltransferase F3GT1"
FT /id="PRO_0000448078"
FT CONFLICT 14
FT /note="V -> L (in Ref. 2; AYJ72756)"
FT /evidence="ECO:0000305"
FT CONFLICT 80
FT /note="R -> G (in Ref. 2; AYJ72756 and 1; ADC34700)"
FT /evidence="ECO:0000305"
FT CONFLICT 108..109
FT /note="TR -> IG (in Ref. 2; AYJ72756)"
FT /evidence="ECO:0000305"
FT CONFLICT 109
FT /note="R -> G (in Ref. 1; ADC34700)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="S -> P (in Ref. 2; AYJ72756 and 1; ADC34700)"
FT /evidence="ECO:0000305"
FT CONFLICT 142
FT /note="Missing (in Ref. 1; ADC34700 and 2; AYJ72756)"
FT /evidence="ECO:0000305"
FT CONFLICT 147..148
FT /note="LS -> FA (in Ref. 2; AYJ72756)"
FT /evidence="ECO:0000305"
FT CONFLICT 272
FT /note="A -> S (in Ref. 2; AYJ72756)"
FT /evidence="ECO:0000305"
FT CONFLICT 378
FT /note="I -> M (in Ref. 1; ADC34700)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 456 AA; 50022 MW; C838086AA888C68E CRC64;
MLITKSSSEC HVAVLAFPFS THPGPLLNLV QRLAVEAPDV IFSFISTAKS NESLFSVPNP
ENIKPYPVWD GVPEGYVFAR KPQEDINLFL KVAAKGFKLA MQAVEVETRR RIGWVMADAF
LWFSSDMAEE RGISWVPIWM SGGACSLSVH LYTDLIRETV GFSGISGRQN ELLKFIPGFS
ELRLGDLPSG VLLGNLKSPF SIMLHKIGQA LPKATTVLIN SFEELDPELN KVLNSNFGKF
LNIGPSNLTS PHPLSNSDEY GCIPWLAKQR SASVAYIGFG SVAKPKPDEV VAIAEALEAS
STPFLWSLRD TSKQYLPEGF LKRTSELGKI VPWAPQVQVL AHSSIGVFIT HCGWNSVLET
IAGGVPMIGR PFFGDHPINT WMVENVWKIG VRVEGGVFTK SSTMRALELV LSHEKGKKLK
DQIGHLRELA LKAVGPKGSS SQNFNNLLEV ITGHNL
