ID   F6CGT_GENTR             Reviewed;         477 AA.
AC   A0A0B6VIJ5;
DT   11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-2015, sequence version 1.
DT   03-AUG-2022, entry version 19.
DE   RecName: Full=UDP-glycosyltransferase UF6CGT1 {ECO:0000303|PubMed:25479084};
DE            Short=GtUF6CGT1 {ECO:0000303|PubMed:25479084};
DE            EC=2.4.1.- {ECO:0000305};
GN   Name=UF6CGT1 {ECO:0000303|PubMed:25479084};
OS   Gentiana triflora (Clustered gentian).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Gentianaceae; Gentianeae; Gentianinae;
OC   Gentiana.
OX   NCBI_TaxID=55190 {ECO:0000312|EMBL:BAQ19550.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=25479084; DOI=10.1016/j.febslet.2014.11.045;
RA   Sasaki N., Nishizaki Y., Yamada E., Tatsuzawa F., Nakatsuka T.,
RA   Takahashi H., Nishihara M.;
RT   "Identification of the glucosyltransferase that mediates direct flavone C-
RT   glucosylation in Gentiana triflora.";
RL   FEBS Lett. 589:182-187(2015).
CC   -!- FUNCTION: UDP-glucose-dependent glucosyltransferase involved in C-
CC       glycosylflavone biosynthesis. Has a strict stereo selectivity and shows
CC       a preference for apigenin and luteolin as substrates, but could not
CC       recognize flavonols, anthocyanins and xanthones as glucosyl acceptors.
CC       No activity with orientin (luteolin 8-C-glucoside) and luteolin 4'-
CC       glucoside. Can use UDP-galactose instead of UDP-glucose as a sugar
CC       donor, but not UDP-glucuronic acid. {ECO:0000269|PubMed:25479084}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in young leaves, with a lower
CC       expression in mature leaves. Higher expression at early stages of
CC       petals development than at the late stages.
CC       {ECO:0000269|PubMed:25479084}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AB985754; BAQ19550.1; -; mRNA.
DR   AlphaFoldDB; A0A0B6VIJ5; -.
DR   SMR; A0A0B6VIJ5; -.
DR   BioCyc; MetaCyc:MON-21889; -.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProt.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   2: Evidence at transcript level;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..477
FT                   /note="UDP-glycosyltransferase UF6CGT1"
FT                   /id="PRO_0000436258"
FT   BINDING         289
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M156"
FT   BINDING         347..348
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M156"
FT   BINDING         365..373
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M156"
FT   BINDING         387..390
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M156"
SQ   SEQUENCE   477 AA;  53517 MW;  4EA0A13ED9D0BA43 CRC64;
     MGSLTNNDNL HIFLVCFIGQ GVVNPMLRLG KAFASKGLLV TLSAPEIVGT EIRKANNLND
     DQPIKVGSGM IRFEFFDDGW ESVNGSKPFD VWVYINHLDQ TGRQKLPIML KKHEETGTPV
     SCLILNPLVP WVADVADSLQ IPCATLWVQS CASFSAYYHY HHGLVPFPTE SEPEIDVQLP
     GMPLLKYDEV PDYLHPRTPY PFFGTNILGQ FKNLSKNFCI LMDTFYELEH EIIDNMCKLC
     PIKPIGPLFK IPKDPSSNGI TGNFMKVDDC KEWLDSRPTS TVVYVSVGSV VYLKQEQVTE
     MAYGILNSEV SFLWVLRPPS KRIGTEPHVL PEEFWEKAGD RGKVVQWSPQ EQVLAHPATV
     GFLTHCGWNS TQEAISSGVP VITFPQFGDQ VTNAKFLVEE FKVGVRLGRG ELENRIITRD
     EVERALREIT SGPKAEEVKE NALKWKKKAE ETVAKGGYSE RNLVGFIEEV ARKTGTK