F86B1_HUMAN
ID F86B1_HUMAN Reviewed; 296 AA.
AC Q8N7N1;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Putative protein N-methyltransferase FAM86B1 {ECO:0000305};
DE EC=2.1.1.- {ECO:0000250|UniProtKB:P47163};
GN Name=FAM86B1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8N7N1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N7N1-2; Sequence=VSP_028646, VSP_028647;
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. EEF2KMT family. {ECO:0000305}.
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DR EMBL; AK098138; BAC05241.1; -; mRNA.
DR EMBL; AC145124; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS59512.1; -. [Q8N7N1-1]
DR RefSeq; NP_001077006.1; NM_001083537.1. [Q8N7N1-1]
DR AlphaFoldDB; Q8N7N1; -.
DR SMR; Q8N7N1; -.
DR BioGRID; 124421; 1.
DR STRING; 9606.ENSP00000407067; -.
DR iPTMnet; Q8N7N1; -.
DR PhosphoSitePlus; Q8N7N1; -.
DR BioMuta; FAM86B1; -.
DR DMDM; 160014086; -.
DR EPD; Q8N7N1; -.
DR jPOST; Q8N7N1; -.
DR MassIVE; Q8N7N1; -.
DR MaxQB; Q8N7N1; -.
DR PeptideAtlas; Q8N7N1; -.
DR PRIDE; Q8N7N1; -.
DR ProteomicsDB; 72309; -. [Q8N7N1-2]
DR Antibodypedia; 76809; 5 antibodies from 5 providers.
DR DNASU; 85002; -.
DR Ensembl; ENST00000340537.9; ENSP00000342610.4; ENSG00000186523.15. [Q8N7N1-2]
DR Ensembl; ENST00000448228.7; ENSP00000407067.2; ENSG00000186523.15. [Q8N7N1-1]
DR Ensembl; ENST00000534520.5; ENSP00000431362.1; ENSG00000186523.15. [Q8N7N1-2]
DR GeneID; 85002; -.
DR KEGG; hsa:85002; -.
DR MANE-Select; ENST00000448228.7; ENSP00000407067.2; NM_001083537.4; NP_001077006.1.
DR UCSC; uc010lse.4; human. [Q8N7N1-1]
DR CTD; 85002; -.
DR GeneCards; FAM86B1; -.
DR HGNC; HGNC:28268; FAM86B1.
DR HPA; ENSG00000186523; Tissue enhanced (brain, pituitary gland).
DR MIM; 616122; gene.
DR neXtProt; NX_Q8N7N1; -.
DR OpenTargets; ENSG00000186523; -.
DR PharmGKB; PA142671859; -.
DR VEuPathDB; HostDB:ENSG00000186523; -.
DR eggNOG; KOG2497; Eukaryota.
DR GeneTree; ENSGT00510000047003; -.
DR HOGENOM; CLU_038942_0_0_1; -.
DR InParanoid; Q8N7N1; -.
DR PhylomeDB; Q8N7N1; -.
DR TreeFam; TF326304; -.
DR PathwayCommons; Q8N7N1; -.
DR SignaLink; Q8N7N1; -.
DR BioGRID-ORCS; 85002; 698 hits in 985 CRISPR screens.
DR GenomeRNAi; 85002; -.
DR Pharos; Q8N7N1; Tdark.
DR PRO; PR:Q8N7N1; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q8N7N1; protein.
DR Bgee; ENSG00000186523; Expressed in right uterine tube and 95 other tissues.
DR ExpressionAtlas; Q8N7N1; baseline and differential.
DR Genevisible; Q8N7N1; HS.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR029426; FAM86_N.
DR InterPro; IPR019410; Methyltransf_16.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR14614; PTHR14614; 2.
DR Pfam; PF14904; FAM86; 1.
DR Pfam; PF10294; Methyltransf_16; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Methyltransferase; Reference proteome;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..296
FT /note="Putative protein N-methyltransferase FAM86B1"
FT /id="PRO_0000307251"
FT BINDING 105
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:Q9H867"
FT BINDING 131..133
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:Q9H867"
FT BINDING 194
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:Q9H867"
FT BINDING 213
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:Q9H867"
FT VAR_SEQ 126..153
FT /note="TVLELGSGAGLTGLAICKMCRPRAYIFS -> RAVLPRSHRVAGRGPAEAGC
FT LPGAQAGS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_028646"
FT VAR_SEQ 154..296
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_028647"
FT CONFLICT 43
FT /note="D -> Y (in Ref. 1; BAC05241)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 296 AA; 32865 MW; A21C313028B95AFB CRC64;
MAPEENAGTE LLLQGFERRF LAVRTLRSFP WQSLEAKLRD SSDSELLRDI LQKTVRHPVC
VKHPPSVKYA WCFLSELIKK SSGGSVTLSK STAIISHGTT GLVTWDAALY LAEWAIENPA
AFINRTVLEL GSGAGLTGLA ICKMCRPRAY IFSDPHSRVL EQLRGNVLLN GLSLEADITG
NLDSPRVTVA QLDWDVAMVH QLSAFQPDVV IAADVLYCPE AIVSLVGVLQ RLAACREHKR
APEVYVAFTV RNPETCQLFT TELGRDGIRW EAEAHHDQKL FPYGEHLEMA MLNLTL
