ID   F9_VACCW                Reviewed;         212 AA.
AC   P24361; Q76ZW7;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   02-JUN-2021, entry version 68.
DE   RecName: Full=Protein F9;
GN   OrderedLocusNames=VACWR048; ORFNames=F9L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2219701; DOI=10.1016/0042-6822(90)90338-r;
RA   Roseman N.A., Slabaugh M.B.;
RT   "The vaccinia virus HindIII F fragment: nucleotide sequence of the left 6.2
RT   kb.";
RL   Virology 178:410-418(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   INTERACTION WITH G4.
RX   PubMed=11983854; DOI=10.1073/pnas.062163799;
RA   Senkevich T.G., White C.L., Koonin E.V., Moss B.;
RT   "Complete pathway for protein disulfide bond formation encoded by
RT   poxviruses.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:6667-6672(2002).
CC   -!- SUBUNIT: Interacts with G4; this interaction involves formation of a
CC       transient disulfide-bonded intermediate, allowing disulfide bond
CC       transfer. {ECO:0000269|PubMed:11983854}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}. Note=Component of the mature virion
CC       (MV) membrane. The mature virion is located in the cytoplasm of
CC       infected cells and is probably released by cell lysis (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae L1 protein family.
CC       {ECO:0000305}.
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DR   EMBL; M34368; AAA48239.1; -; mRNA.
DR   EMBL; AY243312; AAO89327.1; -; Genomic_DNA.
DR   PIR; A36213; A36213.
DR   PDB; 6CJ6; X-ray; 2.10 A; A/B/C/D=1-176.
DR   PDBsum; 6CJ6; -.
DR   SMR; P24361; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR003472; Virion_mem_poxvirus_L1.
DR   Pfam; PF02442; L1R_F9L; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Disulfide bond; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Viral envelope protein; Virion.
FT   CHAIN           1..212
FT                   /note="Protein F9"
FT                   /id="PRO_0000099493"
FT   TOPO_DOM        1..175
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        197..212
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        84
FT                   /note="R -> A (in Ref. 2; AAO89327)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        147
FT                   /note="R -> A (in Ref. 2; AAO89327)"
FT                   /evidence="ECO:0000305"
FT   HELIX           10..24
FT                   /evidence="ECO:0007829|PDB:6CJ6"
FT   STRAND          27..29
FT                   /evidence="ECO:0007829|PDB:6CJ6"
FT   STRAND          36..53
FT                   /evidence="ECO:0007829|PDB:6CJ6"
FT   HELIX           59..74
FT                   /evidence="ECO:0007829|PDB:6CJ6"
FT   HELIX           79..89
FT                   /evidence="ECO:0007829|PDB:6CJ6"
FT   STRAND          93..95
FT                   /evidence="ECO:0007829|PDB:6CJ6"
FT   HELIX           102..113
FT                   /evidence="ECO:0007829|PDB:6CJ6"
FT   STRAND          117..127
FT                   /evidence="ECO:0007829|PDB:6CJ6"
FT   STRAND          133..141
FT                   /evidence="ECO:0007829|PDB:6CJ6"
FT   HELIX           145..158
FT                   /evidence="ECO:0007829|PDB:6CJ6"
SQ   SEQUENCE   212 AA;  23962 MW;  52CFA77A5533144B CRC64;
     MAETKEFKTL YNLFIDSYLQ KLAQHSIPTN VTCAIHIGEV IGQFKNCALR ITNKCMSNSR
     LSFTLMVESF IEVISLLPEK DRRRIAEEIG IDLDDVPSAV SKLEKNCNAY AEVNNIIDIQ
     KLDIGECSAP PGQHMLLQIV NTGSAERNCG LQTIVKSLNK IYVPPIIENR LPYYDPWFLV
     GVAIILVIFT VAICSIRRNL ALKYRYGTFL YV