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FAAH1_CAEEL
ID   FAAH1_CAEEL             Reviewed;         572 AA.
AC   Q17449; H2KY70;
DT   06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 141.
DE   RecName: Full=Fatty acid amide hydrolase 1;
DE            EC=3.5.1.99 {ECO:0000269|PubMed:21562563, ECO:0000269|PubMed:30911178};
DE   AltName: Full=Anandamide amidohydrolase 1;
DE   Flags: Precursor;
GN   Name=faah-1 {ECO:0000312|WormBase:B0218.1};
GN   ORFNames=B0218.1 {ECO:0000312|WormBase:B0218.1};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND TISSUE SPECIFICITY.
RX   PubMed=21562563; DOI=10.1038/nature10007;
RA   Lucanic M., Held J.M., Vantipalli M.C., Klang I.M., Graham J.B.,
RA   Gibson B.W., Lithgow G.J., Gill M.S.;
RT   "N-acylethanolamine signalling mediates the effect of diet on lifespan in
RT   Caenorhabditis elegans.";
RL   Nature 473:226-229(2011).
RN   [3]
RP   FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX   PubMed=23072806; DOI=10.1038/ncomms2136;
RA   Pastuhov S.I., Fujiki K., Nix P., Kanao S., Bastiani M., Matsumoto K.,
RA   Hisamoto N.;
RT   "Endocannabinoid-Goalpha signalling inhibits axon regeneration in
RT   Caenorhabditis elegans by antagonizing Gqalpha-PKC-JNK signalling.";
RL   Nat. Commun. 3:1136-1136(2012).
RN   [4]
RP   FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=30911178; DOI=10.1038/s41589-019-0243-4;
RA   Chen A.L., Lum K.M., Lara-Gonzalez P., Ogasawara D., Cognetta A.B. III,
RA   To A., Parsons W.H., Simon G.M., Desai A., Petrascheck M., Bar-Peled L.,
RA   Cravatt B.F.;
RT   "Pharmacological convergence reveals a lipid pathway that regulates C.
RT   elegans lifespan.";
RL   Nat. Chem. Biol. 15:453-462(2019).
CC   -!- FUNCTION: Catalyzes the hydrolysis of endogenous amidated lipids like
CC       anandamide (AEA or N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine) and
CC       eicosapentaneoyl ethanolamide (EPEA or (5Z,8Z,11Z,14Z,17Z-
CC       eicosapentaenoyl) ethanolamine), as well as other fatty amides, to
CC       their corresponding fatty acids, thereby regulating the signaling
CC       functions of these molecules (PubMed:21562563, PubMed:30911178). EPEA
CC       promotes dauer formation and may constitute a signal of high nutrient
CC       availability (PubMed:21562563). Breakdown of EPEA may promote lifespan
CC       extension when nutrient availability is high (PubMed:21562563).
CC       Facilitates axon regeneration after injury by degradating inhibitory
CC       compounds such as AEA (PubMed:23072806). FAAH cooperates with PM20D1 in
CC       the hydrolysis of amino acid-conjugated fatty acids such as N-fatty
CC       acyl glycine and N-fatty acyl-L-serine, thereby acting as a
CC       physiological regulator of specific subsets of intracellular, but not
CC       of extracellular, N-fatty acyl amino acids (By similarity).
CC       {ECO:0000250|UniProtKB:O08914, ECO:0000269|PubMed:21562563,
CC       ECO:0000269|PubMed:23072806, ECO:0000269|PubMed:30911178}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine =
CC         (5Z,8Z,11Z,14Z)-eicosatetraenoate + ethanolamine;
CC         Xref=Rhea:RHEA:26136, ChEBI:CHEBI:2700, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:32395, ChEBI:CHEBI:57603; EC=3.5.1.99;
CC         Evidence={ECO:0000269|PubMed:21562563, ECO:0000269|PubMed:30911178};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26137;
CC         Evidence={ECO:0000269|PubMed:21562563, ECO:0000269|PubMed:30911178};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenamide + H2O = (9Z)-octadecenoate + NH4(+);
CC         Xref=Rhea:RHEA:26506, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:30823, ChEBI:CHEBI:116314; EC=3.5.1.99;
CC         Evidence={ECO:0000250|UniProtKB:P97612};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26507;
CC         Evidence={ECO:0000250|UniProtKB:P97612};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(5Z,8Z,11Z,14Z,17Z-eicosapentaenoyl) ethanolamine + H2O =
CC         (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + ethanolamine;
CC         Xref=Rhea:RHEA:35519, ChEBI:CHEBI:15377, ChEBI:CHEBI:57603,
CC         ChEBI:CHEBI:58562, ChEBI:CHEBI:71467;
CC         Evidence={ECO:0000269|PubMed:21562563};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35520;
CC         Evidence={ECO:0000269|PubMed:21562563};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-(9Z-hexadecenoyl) ethanolamine = (9Z)-hexadecenoate +
CC         ethanolamine; Xref=Rhea:RHEA:35563, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:32372, ChEBI:CHEBI:57603, ChEBI:CHEBI:71465;
CC         Evidence={ECO:0000269|PubMed:21562563};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35564;
CC         Evidence={ECO:0000269|PubMed:21562563};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-(9Z-octadecenoyl) ethanolamine = (9Z)-octadecenoate +
CC         ethanolamine; Xref=Rhea:RHEA:45060, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:30823, ChEBI:CHEBI:57603, ChEBI:CHEBI:71466;
CC         Evidence={ECO:0000269|PubMed:21562563};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45061;
CC         Evidence={ECO:0000269|PubMed:21562563};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-octadecanoyl ethanolamine = ethanolamine +
CC         octadecanoate; Xref=Rhea:RHEA:63124, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:25629, ChEBI:CHEBI:57603, ChEBI:CHEBI:85299;
CC         Evidence={ECO:0000250|UniProtKB:O08914};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63125;
CC         Evidence={ECO:0000250|UniProtKB:O08914};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-docosanoyl-ethanolamine = docosanoate + ethanolamine;
CC         Xref=Rhea:RHEA:63128, ChEBI:CHEBI:15377, ChEBI:CHEBI:23858,
CC         ChEBI:CHEBI:57603, ChEBI:CHEBI:146186;
CC         Evidence={ECO:0000250|UniProtKB:O08914};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63129;
CC         Evidence={ECO:0000250|UniProtKB:O08914};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-(15Z-tetracosenoyl)-ethanolamine = (15Z)-
CC         tetracosenoate + ethanolamine; Xref=Rhea:RHEA:63144,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:32392, ChEBI:CHEBI:57603,
CC         ChEBI:CHEBI:146187; Evidence={ECO:0000250|UniProtKB:O08914};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63145;
CC         Evidence={ECO:0000250|UniProtKB:O08914};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-hexadecanoylethanolamine = ethanolamine +
CC         hexadecanoate; Xref=Rhea:RHEA:45064, ChEBI:CHEBI:7896,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:57603, ChEBI:CHEBI:71464;
CC         Evidence={ECO:0000269|PubMed:21562563};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45065;
CC         Evidence={ECO:0000269|PubMed:21562563};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-(9Z,12Z-octadecadienoyl)-ethanolamine = (9Z,12Z)-
CC         octadecadienoate + ethanolamine; Xref=Rhea:RHEA:35567,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:30245, ChEBI:CHEBI:57603,
CC         ChEBI:CHEBI:64032; Evidence={ECO:0000269|PubMed:21562563};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35568;
CC         Evidence={ECO:0000269|PubMed:21562563};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoate + glycine = H2O + N-(9Z-
CC         octadecenoyl)glycine; Xref=Rhea:RHEA:51316, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:30823, ChEBI:CHEBI:57305, ChEBI:CHEBI:133992;
CC         Evidence={ECO:0000250|UniProtKB:O08914};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:51318;
CC         Evidence={ECO:0000250|UniProtKB:O08914};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine =
CC         (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycine; Xref=Rhea:RHEA:64108,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:32395, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:59002; Evidence={ECO:0000250|UniProtKB:O08914};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64109;
CC         Evidence={ECO:0000250|UniProtKB:O08914};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-serine =
CC         (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-serine; Xref=Rhea:RHEA:64116,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:32395, ChEBI:CHEBI:33384,
CC         ChEBI:CHEBI:149697; Evidence={ECO:0000250|UniProtKB:O08914};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64117;
CC         Evidence={ECO:0000250|UniProtKB:O08914};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         Vmax=18.3 pmol/min/mg enzyme for the hydrolysis of arachidonoyl
CC         ethanolamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine) (at 37
CC         degrees Celsius) {ECO:0000269|PubMed:30911178};
CC   -!- TISSUE SPECIFICITY: Expressed in the pharynx, some pharyngeal neurons,
CC       the posterior intestine and anal depressor muscles.
CC       {ECO:0000269|PubMed:21562563, ECO:0000269|PubMed:23072806}.
CC   -!- DISRUPTION PHENOTYPE: Viable. Reduction (50 percent) in the frequency
CC       of motoneuron axon regeneration in young adults but not in L4 larvae.
CC       {ECO:0000269|PubMed:23072806}.
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; BX284604; CCD61383.1; -; Genomic_DNA.
DR   PIR; T29753; T29753.
DR   RefSeq; NP_501368.1; NM_068967.5.
DR   AlphaFoldDB; Q17449; -.
DR   SMR; Q17449; -.
DR   BioGRID; 42726; 1.
DR   STRING; 6239.B0218.1a.4; -.
DR   SwissLipids; SLP:000000044; -.
DR   EPD; Q17449; -.
DR   PaxDb; Q17449; -.
DR   PeptideAtlas; Q17449; -.
DR   EnsemblMetazoa; B0218.1.1; B0218.1.1; WBGene00015047.
DR   EnsemblMetazoa; B0218.1.2; B0218.1.2; WBGene00015047.
DR   EnsemblMetazoa; B0218.1.3; B0218.1.3; WBGene00015047.
DR   GeneID; 177613; -.
DR   KEGG; cel:CELE_B0218.1; -.
DR   UCSC; B0218.1a.1; c. elegans.
DR   CTD; 177613; -.
DR   WormBase; B0218.1; CE06684; WBGene00015047; faah-1.
DR   eggNOG; KOG1212; Eukaryota.
DR   GeneTree; ENSGT00970000196322; -.
DR   HOGENOM; CLU_009600_9_3_1; -.
DR   InParanoid; Q17449; -.
DR   OMA; EPWRPEM; -.
DR   OrthoDB; 852596at2759; -.
DR   PhylomeDB; Q17449; -.
DR   PRO; PR:Q17449; -.
DR   Proteomes; UP000001940; Chromosome IV.
DR   Bgee; WBGene00015047; Expressed in larva and 4 other tissues.
DR   GO; GO:0004040; F:amidase activity; IBA:GO_Central.
DR   GO; GO:0103073; F:anandamide amidohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0017064; F:fatty acid amide hydrolase activity; IBA:GO_Central.
DR   GO; GO:0102077; F:oleamide hydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009062; P:fatty acid catabolic process; IBA:GO_Central.
DR   GO; GO:0048680; P:positive regulation of axon regeneration; IMP:UniProtKB.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Hydrolase; Lipid degradation; Lipid metabolism;
KW   Phosphoprotein; Reference proteome; Signal.
FT   SIGNAL          1..14
FT                   /evidence="ECO:0000255"
FT   CHAIN           15..572
FT                   /note="Fatty acid amide hydrolase 1"
FT                   /id="PRO_0000421279"
FT   COILED          32..63
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        139
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P97612"
FT   ACT_SITE        214
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P97612"
FT   ACT_SITE        238
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:P97612"
FT   BINDING         214
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         235..238
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   572 AA;  64001 MW;  4CE781426865D6E9 CRC64;
     MIFYLVLLVL GAIAFYVHFS NNRKKLIERL EIVAQRRRDD LSKNVEQARK AADKLDTQRR
     DWIGSLDFEQ LRDELQRGHV TCVEAIRAYF HKAILAHEKT NAVTCFILDA ERQAEELDEQ
     AKLPYYVKPP LFGVPLSLKE CLKVKGYDTT RGFVQDAYHP ATEDSIQVEH YKKLGLIPFC
     QTNVPQSLLS YNCSNPLFGT TTNPYDSTRT CGGSSGGEGA LIGAGGSLIG IGTDVGGSVR
     IPCHFTGTAG IKPSKMRFAH RGGGASVPGK PLIDANDGPM AKDVKTNVEF LRNVWGDIDF
     QSDRDPYCPP VHWNESVYSS EKKLRVGYYI DDGWFTPTPA LQRAVLESKK HLEAAGHTVI
     PFYPPRLPSV MQLYFRAVCL DGGQYVLNKL LKDIIEPTIR FQVTLWMVPV WIQRILSYPV
     SLVFPRMGML MQSLTRDTFE LREAYADIEA YREEFVGLMM KDNLDVILCP ASIMPAPQHD
     IPSKVVSGVS YTCLYNLLDF GAGVVPVTAV SKSDEEKLIN EYPETDKWYQ ITKKATLGAV
     GMPIGVQVAA PPYREEAVLR TMREIEIAVT GK
 
 
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