FABD_STAAM
ID FABD_STAAM Reviewed; 308 AA.
AC Q99UN8;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Malonyl CoA-acyl carrier protein transacylase;
DE Short=MCT;
DE EC=2.3.1.39;
GN Name=fabD; OrderedLocusNames=SAV1230;
OS Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=158878;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mu50 / ATCC 700699;
RX PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA Hiramatsu K.;
RT "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL Lancet 357:1225-1240(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP];
CC Xref=Rhea:RHEA:41792, Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9685,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:64479,
CC ChEBI:CHEBI:78449; EC=2.3.1.39;
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC -!- SIMILARITY: Belongs to the FabD family. {ECO:0000305}.
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DR EMBL; BA000017; BAB57392.1; -; Genomic_DNA.
DR RefSeq; WP_000047343.1; NC_002758.2.
DR PDB; 3IM9; X-ray; 1.46 A; A=5-308.
DR PDBsum; 3IM9; -.
DR AlphaFoldDB; Q99UN8; -.
DR SMR; Q99UN8; -.
DR World-2DPAGE; 0002:Q99UN8; -.
DR PaxDb; Q99UN8; -.
DR EnsemblBacteria; BAB57392; BAB57392; SAV1230.
DR KEGG; sav:SAV1230; -.
DR HOGENOM; CLU_030558_0_1_9; -.
DR OMA; AANYNCP; -.
DR PhylomeDB; Q99UN8; -.
DR BioCyc; SAUR158878:SAV_RS06625-MON; -.
DR BRENDA; 2.3.1.39; 3352.
DR UniPathway; UPA00094; -.
DR EvolutionaryTrace; Q99UN8; -.
DR PHI-base; PHI:7916; -.
DR Proteomes; UP000002481; Chromosome.
DR GO; GO:0004314; F:[acyl-carrier-protein] S-malonyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.366.10; -; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR024925; Malonyl_CoA-ACP_transAc.
DR InterPro; IPR004410; Malonyl_CoA-ACP_transAc_FabD.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR PIRSF; PIRSF000446; Mct; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SUPFAM; SSF52151; SSF52151; 1.
DR SUPFAM; SSF55048; SSF55048; 1.
DR TIGRFAMs; TIGR00128; fabD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acyltransferase; Fatty acid biosynthesis;
KW Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Transferase.
FT CHAIN 1..308
FT /note="Malonyl CoA-acyl carrier protein transacylase"
FT /id="PRO_0000194224"
FT ACT_SITE 89
FT /evidence="ECO:0000250"
FT ACT_SITE 199
FT /evidence="ECO:0000250"
FT STRAND 5..8
FT /evidence="ECO:0007829|PDB:3IM9"
FT TURN 16..25
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 27..39
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 44..49
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 59..76
FT /evidence="ECO:0007829|PDB:3IM9"
FT STRAND 83..88
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 91..98
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 104..120
FT /evidence="ECO:0007829|PDB:3IM9"
FT STRAND 126..134
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 137..147
FT /evidence="ECO:0007829|PDB:3IM9"
FT STRAND 154..161
FT /evidence="ECO:0007829|PDB:3IM9"
FT STRAND 164..170
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 171..180
FT /evidence="ECO:0007829|PDB:3IM9"
FT TURN 181..185
FT /evidence="ECO:0007829|PDB:3IM9"
FT STRAND 187..191
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 201..206
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 207..214
FT /evidence="ECO:0007829|PDB:3IM9"
FT TURN 229..231
FT /evidence="ECO:0007829|PDB:3IM9"
FT STRAND 233..235
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 238..248
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 255..264
FT /evidence="ECO:0007829|PDB:3IM9"
FT STRAND 267..276
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 278..286
FT /evidence="ECO:0007829|PDB:3IM9"
FT STRAND 288..295
FT /evidence="ECO:0007829|PDB:3IM9"
FT HELIX 298..304
FT /evidence="ECO:0007829|PDB:3IM9"
SQ SEQUENCE 308 AA; 33649 MW; B2FAEF8A200804C3 CRC64;
MSKTAIIFPG QGAQKVGMAQ DLFNNNDQAT EILTSAAKTL DFDILETMFT DEEGKLGETE
NTQPALLTHS SALLAALKNL NPDFTMGHSL GEYSSLVAAD VLSFEDAVKI VRKRGQLMAQ
AFPTGVGSMA AVLGLDFDKV DEICKSLSSD DKIIEPANIN CPGQIVVSGH KALIDELVEK
GKSLGAKRVM PLAVSGPFHS SLMKVIEEDF SSYINQFEWR DAKFPVVQNV NAQGETDKEV
IKSNMVKQLY SPVQFINSTE WLIDQGVDHF IEIGPGKVLS GLIKKINRDV KLTSIQTLED
VKGWNEND
