FABG5_BRANA
ID FABG5_BRANA Reviewed; 317 AA.
AC Q93X68;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=3-oxoacyl-[acyl-carrier-protein] reductase 5, chloroplastic;
DE EC=1.1.1.100;
DE AltName: Full=3-ketoacyl-acyl carrier protein reductase 5;
DE Flags: Precursor; Fragment;
GN Name=bkr1;
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Jet neuf; TISSUE=Embryo;
RA McDonald F.S., White A.J., Elborough K.M., Slabas A.R.;
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+)
CC + NADPH; Xref=Rhea:RHEA:17397, Rhea:RHEA-COMP:9916, Rhea:RHEA-
CC COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:78776, ChEBI:CHEBI:78827; EC=1.1.1.100;
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}. Plastid
CC {ECO:0000250}. Note=And non-photosynthetic plastids. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; AJ243083; CAC41362.1; -; mRNA.
DR AlphaFoldDB; Q93X68; -.
DR SMR; Q93X68; -.
DR UniPathway; UPA00094; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0004316; F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity; IEA:UniProtKB-EC.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR011284; 3oxo_ACP_reduc.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR01830; 3oxo_ACP_reduc; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Fatty acid biosynthesis; Fatty acid metabolism;
KW Lipid biosynthesis; Lipid metabolism; NADP; Oxidoreductase; Plastid;
KW Transit peptide.
FT TRANSIT <1..57
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 58..317
FT /note="3-oxoacyl-[acyl-carrier-protein] reductase 5,
FT chloroplastic"
FT /id="PRO_0000031980"
FT ACT_SITE 224
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 79..103
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 211
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT NON_TER 1
SQ SEQUENCE 317 AA; 33491 MW; 3701327C50ED7104 CRC64;
TTVAATKLTS LKATAGKLGY REICQVRQWA PLKSAMPHFG MLRCATSTVV KAQAQAQATA
TEQTTEEAVP KVESPVVVVT GASRGIGKAI ALSLGKAGCK VLVNYARSAK EAEEVSKQIE
EYGGEAITFG GDVSKEADVD SMMKTAVDKW GTIDVVVNNA GITRDTLLIR MKKSQWDEVI
DLNLTGVFLC TQAATKIMMK KRKGRIINIA SVVGLIGNIG QANYAAAKAG VIGFSKTAAR
EGASRNINVN VVCPGFIASD MTAKLGEDME KKILGTIPLG RYGQPEYVAG LVEFLALSPA
SSYITGHTFS IHGGFAI
