FABG6_BRANA
ID FABG6_BRANA Reviewed; 201 AA.
AC P27582;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 3.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=3-oxoacyl-[acyl-carrier-protein] reductase;
DE EC=1.1.1.100;
DE AltName: Full=3-ketoacyl-acyl carrier protein reductase;
DE Flags: Fragments;
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708;
RN [1]
RP PROTEIN SEQUENCE OF 35-62 AND 81-84.
RC TISSUE=Seed;
RX PubMed=1562581; DOI=10.1016/0167-4838(92)90263-d;
RA Sheldon P.S., Kekwick R.G.O., Smith C.G., Sidebottom C.M., Slabas A.R.;
RT "3-oxoacyl-[ACP] reductase from oilseed rape (Brassica napus).";
RL Biochim. Biophys. Acta 1120:151-159(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 63-201, AND PARTIAL PROTEIN SEQUENCE.
RC TISSUE=Seed;
RX PubMed=1575676; DOI=10.1042/bj2830321;
RA Slabas A.R., Chase D., Nishida I., Murata N., Sidebottom C.M., Safford R.,
RA Sheldon P.S., Kekwick R.G.O., Hardie D.G., Mackintosh R.W.;
RT "Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein)
RT reductase. Sequence identities with the nodG-gene product of the nitrogen-
RT fixing soil bacterium Rhizobium meliloti.";
RL Biochem. J. 283:321-326(1992).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+)
CC + NADPH; Xref=Rhea:RHEA:17397, Rhea:RHEA-COMP:9916, Rhea:RHEA-
CC COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:78776, ChEBI:CHEBI:78827; EC=1.1.1.100;
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC -!- SUBUNIT: Homotetramer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast. Note=And non-photosynthetic
CC plastids.
CC -!- TISSUE SPECIFICITY: Embryo and leaf tissues.
CC -!- MISCELLANEOUS: Exhibits a marked preference for acyl-carrier protein
CC derivatives over CoA derivatives as substrates.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; X64463; CAA45793.1; -; mRNA.
DR PIR; S22417; S22417.
DR UniPathway; UPA00094; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0004316; F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; Fatty acid biosynthesis;
KW Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; NADP;
KW Oxidoreductase; Plastid.
FT CHAIN 1..201
FT /note="3-oxoacyl-[acyl-carrier-protein] reductase"
FT /id="PRO_0000054661"
FT ACT_SITE 108
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 95
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT NON_CONS 27..28
FT /evidence="ECO:0000305"
FT NON_CONS 34..35
FT /evidence="ECO:0000305"
FT NON_CONS 62..63
FT /evidence="ECO:0000305"
SQ SEQUENCE 201 AA; 21047 MW; FD51B2E369D2D967 CRC64;
ATAQEQPEGE APDKVESPVV XXXEAXIVLV NYAREADVDA MMKDAVDYWG QIDVIANNAG
ITVIALNLTG VFLCSQAATK IMMKKRKGRI INIASVVGLI GNIGQANYAA AKAGVIGFSK
TAAREGASRN INVNVVCPGF IASEMTAKLG EDMEKKILGT IPLGRYGQPE DVAGLVEFLA
LSPAASYITG QTFTIDGGIA I
