ID   AI2A_DICDI              Reviewed;         309 AA.
AC   O21043;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Intron-encoded DNA endonuclease ai2a;
DE            EC=3.1.-.-;
GN   Name=ai2a; ORFNames=DDB_G0294421;
OS   Dictyostelium discoideum (Slime mold).
OG   Mitochondrion.
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AX3;
RX   PubMed=9000384; DOI=10.1007/s002940050179;
RA   Ogawa S., Matsuo K., Angata K., Yanagisawa K., Tanaka Y.;
RT   "Group-I introns in the cytochrome c oxidase genes of Dictyostelium
RT   discoideum: two related ORFs in one loop of a group-I intron, a cox1/2
RT   hybrid gene and an unusually large cox3 gene.";
RL   Curr. Genet. 31:80-88(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX3;
RX   PubMed=10821186; DOI=10.1007/pl00008685;
RA   Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K.,
RA   Morio T., Urushihara H., Yanagisawa K., Tanaka Y.;
RT   "The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene
RT   content and genome organization.";
RL   Mol. Gen. Genet. 263:514-519(2000).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=9210597; DOI=10.1016/s0378-1119(97)00050-4;
RA   Ogawa S., Naito K., Angata K., Morio T., Urushihara H., Tanaka Y.;
RT   "A site-specific DNA endonuclease specified by one of two ORFs encoded by a
RT   group I intron in Dictyostelium discoideum mitochondrial DNA.";
RL   Gene 191:115-121(1997).
CC   -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC       Cleaves only one strand of intronless DNA sequence at the site which
CC       coincides with the I-SceII cleavage recognition site.
CC       {ECO:0000269|PubMed:9210597}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:9210597}.
CC   -!- MISCELLANEOUS: The expression of ai2a in E.coli causes growth
CC       inhibition and degradation of genomic DNA.
CC   -!- SIMILARITY: Belongs to the LAGLIDADG endonuclease family.
CC       {ECO:0000305}.
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DR   EMBL; D50297; BAA21124.1; -; Genomic_DNA.
DR   EMBL; AB000109; BAA78056.1; -; Genomic_DNA.
DR   PIR; T43752; T43752.
DR   RefSeq; NP_050074.1; NC_000895.1.
DR   AlphaFoldDB; O21043; -.
DR   SMR; O21043; -.
DR   REBASE; 3189; I-DdiI.
DR   PRIDE; O21043; -.
DR   GeneID; 2193940; -.
DR   KEGG; ddi:DidioMp07; -.
DR   dictyBase; DDB_G0294421; ai2a.
DR   InParanoid; O21043; -.
DR   OMA; NGHIRHP; -.
DR   PhylomeDB; O21043; -.
DR   PRO; PR:O21043; -.
DR   Proteomes; UP000002195; Mitochondrion.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0004520; F:endodeoxyribonuclease activity; IDA:dictyBase.
DR   GO; GO:0006314; P:intron homing; TAS:dictyBase.
DR   Gene3D; 3.10.28.10; -; 2.
DR   InterPro; IPR027434; Homing_endonucl.
DR   SUPFAM; SSF55608; SSF55608; 2.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Intron homing; Mitochondrion; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..309
FT                   /note="Intron-encoded DNA endonuclease ai2a"
FT                   /id="PRO_0000386627"
SQ   SEQUENCE   309 AA;  37116 MW;  2F7E4085CCF2FA30 CRC64;
     MKKEEKKENK KREQNQISSK KWNEWLAGLI DGAGKFTLSK SGYANLDILL ELRDEKCHIE
     LKQRFGGEIK RKETTLIRYR VHRLYGIRQL IACVNGHIRH PLRKIEFKEI CMHYNVEYKE
     PETLTFANGW LSGYFDAVGL IEFSTEQDQL YLAFRHKKNT MLNELEIQKC LGVNLTCEQN
     GCSCESVTLY KINKKTVLSL YKYFKKYRLR SSIKTTQILL IPQFYEIQNE TKEINDMQVK
     QKLWKQFFKK WYVDEMVKYE SVKLANYYKS KTSPEKRVKK LIKLKFHRNE RIRKRAERLE
     LQKEKEKKA