AI2B_DICDI
ID AI2B_DICDI Reviewed; 262 AA.
AC O21044; Q9T4Y1;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Intron-encoded DNA endonuclease ai2b;
DE EC=3.1.-.-;
GN Name=ai2b; ORFNames=DDB_G0294423;
OS Dictyostelium discoideum (Slime mold).
OG Mitochondrion.
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AX3;
RX PubMed=9000384; DOI=10.1007/s002940050179;
RA Ogawa S., Matsuo K., Angata K., Yanagisawa K., Tanaka Y.;
RT "Group-I introns in the cytochrome c oxidase genes of Dictyostelium
RT discoideum: two related ORFs in one loop of a group-I intron, a cox1/2
RT hybrid gene and an unusually large cox3 gene.";
RL Curr. Genet. 31:80-88(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX3;
RX PubMed=10821186; DOI=10.1007/pl00008685;
RA Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K.,
RA Morio T., Urushihara H., Yanagisawa K., Tanaka Y.;
RT "The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene
RT content and genome organization.";
RL Mol. Gen. Genet. 263:514-519(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 89-262.
RC STRAIN=AX3;
RX PubMed=9186775; DOI=10.1016/s0005-2728(97)00010-8;
RA Pellizzari R., Anjard C., Bisson R.;
RT "Subunits I and II of Dictyostelium cytochrome c oxidase are specified by a
RT single open reading frame transcribed into a large polycistronic RNA.";
RL Biochim. Biophys. Acta 1320:1-7(1997).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=9210597; DOI=10.1016/s0378-1119(97)00050-4;
RA Ogawa S., Naito K., Angata K., Morio T., Urushihara H., Tanaka Y.;
RT "A site-specific DNA endonuclease specified by one of two ORFs encoded by a
RT group I intron in Dictyostelium discoideum mitochondrial DNA.";
RL Gene 191:115-121(1997).
CC -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:9210597}.
CC -!- SIMILARITY: Belongs to the LAGLIDADG endonuclease family.
CC {ECO:0000305}.
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DR EMBL; D50297; BAA21125.1; -; Genomic_DNA.
DR EMBL; AB000109; BAA78057.1; -; Genomic_DNA.
DR EMBL; X81884; CAA57468.1; -; Genomic_DNA.
DR PIR; T43753; T43753.
DR RefSeq; NP_050075.1; NC_000895.1.
DR AlphaFoldDB; O21044; -.
DR SMR; O21044; -.
DR GeneID; 2193941; -.
DR KEGG; ddi:DidioMp08; -.
DR dictyBase; DDB_G0294423; ai2b.
DR InParanoid; O21044; -.
DR PhylomeDB; O21044; -.
DR PRO; PR:O21044; -.
DR Proteomes; UP000002195; Mitochondrion.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR Gene3D; 3.10.28.10; -; 2.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR004860; LAGLIDADG_2.
DR Pfam; PF00961; LAGLIDADG_1; 1.
DR SUPFAM; SSF55608; SSF55608; 2.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Intron homing; Mitochondrion; Nuclease;
KW Reference proteome.
FT CHAIN 1..262
FT /note="Intron-encoded DNA endonuclease ai2b"
FT /id="PRO_0000386628"
SQ SEQUENCE 262 AA; 30614 MW; B7397DC414342035 CRC64;
MRIQIEKDQL GWVAGLLDSC GNIMFSKTHV SGNIEIKLKL TDYECLENMK TKYGGAVKVS
RTGTRYRLNH SEGLKKLLED INGQIRIPVR VKKVKEVCEK NNIEIKYPDK ELSLDTAWLS
GFFDGVGSIV INQTTLENKK QLVLTFNQKN NQILIKIQRI VGGNFYINTN TQSGKYKYAL
YFTSKEEILQ LYEYFKKYPS RAYKNKRIGL IPQYYELQTL MTSIHDNQLQ ETLWKKFMIQ
WDYKEIDEIQ SGEILKQKGV IR
