AI2_DEBHA
ID AI2_DEBHA Reviewed; 657 AA.
AC A9RAH7;
DT 25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 1.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Probable intron-encoded endonuclease aI2;
DE Contains:
DE RecName: Full=Truncated non-functional cytochrome oxidase 1;
DE Contains:
DE RecName: Full=Intron-encoded endonuclease aI2;
DE EC=3.1.-.-;
DE Flags: Precursor;
GN Name=aI2;
OS Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990
OS / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii).
OG Mitochondrion.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Debaryomyces.
OX NCBI_TaxID=284592;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968;
RX PubMed=18673395; DOI=10.1111/j.1567-1364.2008.00409.x;
RA Sacerdot C., Casaregola S., Lafontaine I., Tekaia F., Dujon B.,
RA Ozier-Kalogeropoulos O.;
RT "Promiscuous DNA in the nuclear genomes of hemiascomycetous yeasts.";
RL FEMS Yeast Res. 8:846-857(2008).
CC -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion. Membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- PTM: The mature protein may arise from proteolytic cleavage of an in-
CC frame translation of COX1 exons 1 and 2 plus intron 2, containing the
CC aI2 open reading frame.
CC -!- SIMILARITY: In the C-terminal section; belongs to the LAGLIDADG
CC endonuclease family. {ECO:0000305}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the heme-copper
CC respiratory oxidase family. {ECO:0000305}.
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DR EMBL; DQ508940; ABF58078.1; -; Genomic_DNA.
DR RefSeq; YP_001621429.1; NC_010166.1.
DR AlphaFoldDB; A9RAH7; -.
DR SMR; A9RAH7; -.
DR STRING; 284592.A9RAH7; -.
DR GeneID; 5845853; -.
DR InParanoid; A9RAH7; -.
DR Proteomes; UP000000599; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0009060; P:aerobic respiration; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR Gene3D; 1.20.210.10; -; 1.
DR Gene3D; 3.10.28.10; -; 2.
DR InterPro; IPR023616; Cyt_c_oxase-like_su1_dom.
DR InterPro; IPR036927; Cyt_c_oxase-like_su1_sf.
DR InterPro; IPR000883; Cyt_C_Oxase_1.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR004860; LAGLIDADG_2.
DR PANTHER; PTHR10422; PTHR10422; 1.
DR Pfam; PF00115; COX1; 1.
DR Pfam; PF00961; LAGLIDADG_1; 2.
DR PRINTS; PR01165; CYCOXIDASEI.
DR SUPFAM; SSF55608; SSF55608; 2.
DR SUPFAM; SSF81442; SSF81442; 1.
DR PROSITE; PS50855; COX1; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Intron homing; Membrane; Mitochondrion; Nuclease;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..?
FT /note="Truncated non-functional cytochrome oxidase 1"
FT /id="PRO_0000355037"
FT CHAIN ?..657
FT /note="Intron-encoded endonuclease aI2"
FT /id="PRO_0000355038"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..245
FT /note="COX1 exons 1 to 2 encoded"
FT REGION 246..657
FT /note="COX1 intron 2 encoded"
SQ SEQUENCE 657 AA; 75166 MW; 9302A33F1CA1CCB4 CRC64;
MKQMSYVTRW LYSTSHKDIG MTYLGFGMLS AMMGTGMSVM MRMELSNGNS QFFHGNNQAF
NVMMSGHALL MMFFFIMPVW MGAFGNFFLP MLMGAADMAF ARLNNISFWC LPPALVCMVC
SVLMEQGAGT GFTTYPPLSS MSAHSGPSVD LAMFAMHLTS MSSLLGAMNF MVTVLNMRTM
GLHMVNMPLF AWAMFLTAML LLLSLPVLTA AVTLLLMDRN FNTGFYEVGA GGDPVTYEHL
FWFFGQWWPT NYVNNLEYCA MCWETYNKMY FIMLLITMYM STNLLANMVK MLMTNRSNQQ
VTKNRMNKPH ALRGDTSPKV RHKMFMNTMS KKSHALRRDT SLFSFPKNDK VRHMYDMNSK
RYKSYLMGTS ETTSTKSLKE MNMPSQRTGN PAITGTNLKE NKNTMENSFN QWLAGLMDGD
GCFGITQNKY TNCEMTVALE DEKTLRIIQN KFGGSMKLRS GAKAIRYRLH NQKGMINTMN
AINGNMRHSK RLVQLHKVCS LLNMPVLEPM ILTKNNSWLT GFFDADGTMN FSFKSSCHSA
GGYTSKGKVS NHPQLTISVT NKYLQDVLPF KEMLGGNIYF DKSQNGYYKW SMQSKKDILN
FVDYIKLNPS KTVKLNRMLL CNLYYDLKDL KSYMLNDNNM LQNKAWIKFE NKWNKKF
