AI3_DEBHA
ID AI3_DEBHA Reviewed; 687 AA.
AC A9RAH6;
DT 25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 1.
DT 25-MAY-2022, entry version 70.
DE RecName: Full=Probable intron-encoded endonuclease aI3;
DE Contains:
DE RecName: Full=Truncated non-functional cytochrome oxidase 1;
DE Contains:
DE RecName: Full=Intron-encoded endonuclease aI3;
DE EC=3.1.-.-;
DE Flags: Precursor;
GN Name=aI3;
OS Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990
OS / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii).
OG Mitochondrion.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Debaryomyces.
OX NCBI_TaxID=284592;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968;
RX PubMed=18673395; DOI=10.1111/j.1567-1364.2008.00409.x;
RA Sacerdot C., Casaregola S., Lafontaine I., Tekaia F., Dujon B.,
RA Ozier-Kalogeropoulos O.;
RT "Promiscuous DNA in the nuclear genomes of hemiascomycetous yeasts.";
RL FEMS Yeast Res. 8:846-857(2008).
CC -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion. Membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- PTM: The mature protein may arise from proteolytic cleavage of an in-
CC frame translation of COX1 exons 1 to 3 plus intron 3, containing the
CC aI3 open reading frame.
CC -!- SIMILARITY: In the C-terminal section; belongs to the LAGLIDADG
CC endonuclease family. {ECO:0000305}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the heme-copper
CC respiratory oxidase family. {ECO:0000305}.
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DR EMBL; DQ508940; ABF58077.1; -; Genomic_DNA.
DR RefSeq; YP_001621428.1; NC_010166.1.
DR AlphaFoldDB; A9RAH6; -.
DR SMR; A9RAH6; -.
DR STRING; 284592.A9RAH6; -.
DR GeneID; 5845853; -.
DR InParanoid; A9RAH6; -.
DR Proteomes; UP000000599; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0009060; P:aerobic respiration; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR Gene3D; 1.20.210.10; -; 1.
DR Gene3D; 3.10.28.10; -; 2.
DR InterPro; IPR023616; Cyt_c_oxase-like_su1_dom.
DR InterPro; IPR036927; Cyt_c_oxase-like_su1_sf.
DR InterPro; IPR000883; Cyt_C_Oxase_1.
DR InterPro; IPR023615; Cyt_c_Oxase_su1_BS.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR004860; LAGLIDADG_2.
DR PANTHER; PTHR10422; PTHR10422; 1.
DR Pfam; PF00115; COX1; 1.
DR Pfam; PF00961; LAGLIDADG_1; 2.
DR PRINTS; PR01165; CYCOXIDASEI.
DR SUPFAM; SSF55608; SSF55608; 2.
DR SUPFAM; SSF81442; SSF81442; 1.
DR PROSITE; PS50855; COX1; 1.
DR PROSITE; PS00077; COX1_CUB; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Intron homing; Membrane; Mitochondrion; Nuclease;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..?
FT /note="Truncated non-functional cytochrome oxidase 1"
FT /id="PRO_0000355039"
FT CHAIN ?..687
FT /note="Intron-encoded endonuclease aI3"
FT /id="PRO_0000355040"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..374
FT /note="COX1 exons 1 to 3 encoded"
FT REGION 375..687
FT /note="COX1 intron 3 encoded"
SQ SEQUENCE 687 AA; 78619 MW; 83FBA7AB0A329CD5 CRC64;
MKQMSYVTRW LYSTSHKDIG MTYLGFGMLS AMMGTGMSVM MRMELSNGNS QFFHGNNQAF
NVMMSGHALL MMFFFIMPVW MGAFGNFFLP MLMGAADMAF ARLNNISFWC LPPALVCMVC
SVLMEQGAGT GFTTYPPLSS MSAHSGPSVD LAMFAMHLTS MSSLLGAMNF MVTVLNMRTM
GLHMVNMPLF AWAMFLTAML LLLSLPVLTA AVTLLLMDRN FNTGFYEVGA GGDPVTYEHL
FWFFGHPEVY ILMMPGFGVM SHMVSTYSKK PMFGEMGMLY AMGSIGFLGF LVWSHHMFVV
GLDIDSRAYF TSATMVIAVP TGIKIFSWLA TIYGGELRLG VPMLFALGFL FLFTMGGLTG
VMTSNASMDV AFHDRIFIYY VSFFLYTLYN MYNNYTNNNT AHKGRHCANA SYNNDINNLV
SFMDKNEYIK MFWVGLMDGD GSMQVNHWRK KNLQYRLIMK TSNLESNYNM LMLMAKVMGG
TVRISKKKDN VMWVVDSKES IKDMMKMFDK YPLLTSRKMC QINFMKKCMK LNYMPAEGGY
PREAGTDWYL LNRNYKYNDQ LNMINTFNLK LKNKSFMNAL PEGHGMHHMV KHSEYFKCWL
SGFMEAEGCF SIRLNKSNSF SMGQNDDFYL MEEIKQFFNA TNSVRNSYKN FYCLEIYKRI
MLNNIITHCS NYPLLGEKKE SLMKFLK