ID   AI3_KLULA               Reviewed;         396 AA.
AC   Q6DN58;
DT   25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Probable intron-encoded endonuclease aI3;
DE            EC=3.1.-.-;
GN   Name=aI3;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OG   Mitochondrion.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 76492 / CBS 2359/152 / CLIB 210;
RX   PubMed=15691736; DOI=10.1016/j.femsyr.2004.09.003;
RA   Zivanovic Y., Wincker P., Vacherie B., Bolotin-Fukuhara M., Fukuhara H.;
RT   "Complete nucleotide sequence of the mitochondrial DNA from Kluyveromyces
RT   lactis.";
RL   FEMS Yeast Res. 5:315-322(2005).
CC   -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- MISCELLANEOUS: Encoded within intron 3 of COX1.
CC   -!- SIMILARITY: Belongs to the LAGLIDADG endonuclease family.
CC       {ECO:0000305}.
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DR   EMBL; AY654900; AAT64957.1; -; Genomic_DNA.
DR   RefSeq; YP_054501.1; NC_006077.1.
DR   AlphaFoldDB; Q6DN58; -.
DR   SMR; Q6DN58; -.
DR   GeneID; 2914076; -.
DR   InParanoid; Q6DN58; -.
DR   Proteomes; UP000000598; Mitochondrion.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.28.10; -; 2.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR004860; LAGLIDADG_2.
DR   Pfam; PF00961; LAGLIDADG_1; 2.
DR   SUPFAM; SSF55608; SSF55608; 2.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Intron homing; Mitochondrion; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..396
FT                   /note="Probable intron-encoded endonuclease aI3"
FT                   /id="PRO_0000415147"
FT   REGION          51..90
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   396 AA;  47401 MW;  58EF830AFDBA9CC6 CRC64;
     MNIIIIILYI SYIYTYPFSN GKYCNLYNPT TFYKINNILY SIKIYNINNN TNNTNNNNPA
     DSSSYESRMR AAGNSNSNSN SNSDSNINNT NNINNTNNIY NINNNINNNN NINNNYINNS
     IPCGSYPQGR WTAGSEFYKM LFVGLFDSNG NIQINKYKSK YLQFRLIIKL NNNKENINIL
     NNIKHYLKGN ININNNYVIW IINDIKNINN LIKLFNKYPL ITINKKLQLA FIKSIYYIYK
     NNRNLAINLY LKDRNNKYNP NLYKYYKDIN YTKINYFSPA NFHKKFAGIN INININNNYN
     HNYINVWFLG FIENKGKFII RKNNNNSFLF YINDKHLIEF LKNYFNIKNK LIYKNNIYIL
     EVYNKYYINI FIKFFNKYNL QGYKYIEYIK WKKINI