ID   AI3_USTMA               Reviewed;         174 AA.
AC   Q0H8X9;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=Probable intron-encoded DNA endonuclease aI3;
DE            EC=3.1.-.-;
GN   Name=aI3;
OS   Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OG   Mitochondrion.
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX   NCBI_TaxID=237631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RA   Kennell J.C., Boehmer C.;
RT   "Annotation of mitochondrial genome of Ustilago maydis and comparative
RT   analysis of basidiomycete mtDNAs.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=17080091; DOI=10.1038/nature05248;
RA   Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA   Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA   Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA   Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA   Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA   Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA   Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA   Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA   Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA   Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA   Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA   Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA   Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA   Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA   Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA   Birren B.W.;
RT   "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT   maydis.";
RL   Nature 444:97-101(2006).
CC   -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- MISCELLANEOUS: Encoded within intron 3 of COX1.
CC   -!- SIMILARITY: To endonucleases of group I introns of fungi and phage.
CC       {ECO:0000305}.
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DR   EMBL; DQ157700; AAZ67027.1; -; Genomic_DNA.
DR   EMBL; AACP01000277; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; YP_762693.1; NC_008368.1.
DR   AlphaFoldDB; Q0H8X9; -.
DR   GeneID; 4308276; -.
DR   InParanoid; Q0H8X9; -.
DR   Proteomes; UP000000561; Mitochondrion.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR   InterPro; IPR003647; Intron_nuc_1_rpt.
DR   SMART; SM00497; IENR1; 2.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Intron homing; Mitochondrion; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..174
FT                   /note="Probable intron-encoded DNA endonuclease aI3"
FT                   /id="PRO_0000271155"
SQ   SEQUENCE   174 AA;  20083 MW;  7955A84FAB13A5B1 CRC64;
     MVLEQYFLLL PEFNLNTIRV SNNPSGSAAK PLFMYNRDGS ICYYKSTKQV DFVRTFGIHK
     VTFMKHLEQG TYYLNKYLFS MEELPNAEQS DMTVEQVATM LDIDRVTNNK NKTLVSFSVA
     VTLINIETKE EHKFPSLGTA VKFLKNGSYP ADQRTLVKRI NTNIPYHGFT CHKY