ID   AI4_DICDI               Reviewed;         159 AA.
AC   Q9TGM4; O21046; O21151;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Intron-encoded endonuclease ai4;
DE            EC=3.1.-.-;
DE   AltName: Full=Cox1/2 intron 3 orf;
GN   Name=ai4; ORFNames=DDB_G0294080;
OS   Dictyostelium discoideum (Slime mold).
OG   Mitochondrion.
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AX3;
RX   PubMed=9186775; DOI=10.1016/s0005-2728(97)00010-8;
RA   Pellizzari R., Anjard C., Bisson R.;
RT   "Subunits I and II of Dictyostelium cytochrome c oxidase are specified by a
RT   single open reading frame transcribed into a large polycistronic RNA.";
RL   Biochim. Biophys. Acta 1320:1-7(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AX3;
RX   PubMed=9000384; DOI=10.1007/s002940050179;
RA   Ogawa S., Matsuo K., Angata K., Yanagisawa K., Tanaka Y.;
RT   "Group-I introns in the cytochrome c oxidase genes of Dictyostelium
RT   discoideum: two related ORFs in one loop of a group-I intron, a cox1/2
RT   hybrid gene and an unusually large cox3 gene.";
RL   Curr. Genet. 31:80-88(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX3;
RX   PubMed=10821186; DOI=10.1007/pl00008685;
RA   Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K.,
RA   Morio T., Urushihara H., Yanagisawa K., Tanaka Y.;
RT   "The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene
RT   content and genome organization.";
RL   Mol. Gen. Genet. 263:514-519(2000).
RN   [4]
RP   IDENTIFICATION.
RX   PubMed=9210597; DOI=10.1016/s0378-1119(97)00050-4;
RA   Ogawa S., Naito K., Angata K., Morio T., Urushihara H., Tanaka Y.;
RT   "A site-specific DNA endonuclease specified by one of two ORFs encoded by a
RT   group I intron in Dictyostelium discoideum mitochondrial DNA.";
RL   Gene 191:115-121(1997).
CC   -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the LAGLIDADG endonuclease family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA21127.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=CAA57470.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; X81884; CAA57470.1; ALT_INIT; Genomic_DNA.
DR   EMBL; D50297; BAA21127.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AB000109; BAA78059.1; -; Genomic_DNA.
DR   PIR; T43755; T43755.
DR   RefSeq; NP_050077.1; NC_000895.1.
DR   AlphaFoldDB; Q9TGM4; -.
DR   SMR; Q9TGM4; -.
DR   GeneID; 2193943; -.
DR   KEGG; ddi:DidioMp10; -.
DR   dictyBase; DDB_G0294080; ai4.
DR   InParanoid; Q9TGM4; -.
DR   PRO; PR:Q9TGM4; -.
DR   Proteomes; UP000002195; Mitochondrion.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.28.10; -; 2.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR004860; LAGLIDADG_2.
DR   Pfam; PF03161; LAGLIDADG_2; 1.
DR   SUPFAM; SSF55608; SSF55608; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Intron homing; Mitochondrion; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..159
FT                   /note="Intron-encoded endonuclease ai4"
FT                   /id="PRO_0000410723"
FT   CONFLICT        157..159
FT                   /note="GIK -> E (in Ref. 1; CAA57470)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   159 AA;  19358 MW;  D037F8BE0E927762 CRC64;
     MIYEFFNKNG YCTNNIPRKY IVKLKHNDDV KDFERYEFVT YTFRSFKYIY EMFYKKQKKV
     LHLEKIKEYL DERALAHWIM DDGGYTGAGI RIATNAFTLK EVMFLKELLE DKFKLKCTIQ
     EIYIKDKWSI YITKDSMTHL ISIIKPYMHP SMYYKLGIK