AI4_USTMA
ID AI4_USTMA Reviewed; 530 AA.
AC Q0H8Y0;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=Probable intron-encoded endonuclease aI4;
DE Contains:
DE RecName: Full=Truncated non-functional cytochrome oxidase 1;
DE Contains:
DE RecName: Full=Intron-encoded endonuclease aI4;
DE EC=3.1.-.-;
DE Flags: Precursor;
GN Name=aI4;
OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OG Mitochondrion.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=237631;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RA Kennell J.C., Boehmer C.;
RT "Annotation of mitochondrial genome of Ustilago maydis and comparative
RT analysis of basidiomycete mtDNAs.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=17080091; DOI=10.1038/nature05248;
RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA Birren B.W.;
RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT maydis.";
RL Nature 444:97-101(2006).
CC -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion. Membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- PTM: The mature protein may arise from proteolytic cleavage of an in-
CC frame translation of COX1 exons 1 to 4 plus intron 4, containing the
CC aI4 open reading frame.
CC -!- SIMILARITY: In the C-terminal section; belongs to the LAGLIDADG
CC endonuclease family. {ECO:0000305}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the heme-copper
CC respiratory oxidase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAZ67026.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ157700; AAZ67026.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AACP01000277; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; YP_762692.1; NC_008368.1.
DR AlphaFoldDB; Q0H8Y0; -.
DR SMR; Q0H8Y0; -.
DR GeneID; 4308276; -.
DR InParanoid; Q0H8Y0; -.
DR Proteomes; UP000000561; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005751; C:mitochondrial respiratory chain complex IV; IBA:GO_Central.
DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IBA:GO_Central.
DR GO; GO:0022904; P:respiratory electron transport chain; IBA:GO_Central.
DR Gene3D; 1.20.210.10; -; 1.
DR Gene3D; 3.10.28.10; -; 2.
DR InterPro; IPR023616; Cyt_c_oxase-like_su1_dom.
DR InterPro; IPR036927; Cyt_c_oxase-like_su1_sf.
DR InterPro; IPR000883; Cyt_C_Oxase_1.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR004860; LAGLIDADG_2.
DR PANTHER; PTHR10422; PTHR10422; 1.
DR Pfam; PF00115; COX1; 1.
DR Pfam; PF00961; LAGLIDADG_1; 2.
DR PRINTS; PR01165; CYCOXIDASEI.
DR SUPFAM; SSF55608; SSF55608; 2.
DR SUPFAM; SSF81442; SSF81442; 1.
DR PROSITE; PS50855; COX1; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Intron homing; Membrane; Mitochondrion; Nuclease;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..?
FT /note="Truncated non-functional cytochrome oxidase 1"
FT /id="PRO_0000271156"
FT CHAIN ?..530
FT /note="Intron-encoded endonuclease aI4"
FT /id="PRO_0000271157"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..204
FT /note="COX1 exons 1 to 4 encoded"
FT REGION 205..530
FT /note="COX1 intron 4 encoded"
SQ SEQUENCE 530 AA; 59676 MW; 8E03615FC83DAE76 CRC64;
MVRWLYSTNA KDIGTLYLIF AVFAAMIGTA FSVLIRMELA APGVQYLNGD HQLYNVIITA
HAFVMIFFMV MPAMVGGFGN YLVPVMIGAP DMAFPRLNNI SFWLLPPSLI LLLASAFVEQ
GAGTGWTVYP PLSGLQSHSG GSVDLAIFSL HLSGISSMLG AMNFITTVLN MRNPGMTLHK
LPLFVWAIFV TAILLLLSLP VLAGKFVPAL NLAVCWELFS SVFSKIIDVK DDQQVTYESY
NFIWNLNDCA PELSISHMSS LLFFGSYLAG LIEGDGTIVV PKQERSAKGK RNYPSVQIVF
QLKDFPLCQI VQKLIGHGTI SKKKQAAAYI LTINNIQGLI ELSNLINGKI RGPKYYQFVL
LVEYLNKKCP NLNLKPAGFN KTPLGFDSWL SGFIEADGSF QVRTSLSSKY PRLSLSFELV
QSRITHYGFT MFTIMQEIAI FLETNVNEIR SERKNPQYRL RTSSLKTNKN IRDYLVKYPL
KGTKYLDFKD WCKVLSYFEQ GTHMDNTARI VEIKSQMNQR RTVYNWDHLQ